Gram Negative Flashcards
Gram (-) Diplococci that is aeorbic and can utilize maltose positive
N. Meningitidis
Comma shaped rods that grows in alkaline media
Vibrio cholerae
Gram negative bacilli that ferment lactose faster
Klebsiella
E coli
Ebterobacter
Transmission of Neisseria meningitidis
Respiratory droplets
• in close quarters (camps, dormitories)
• carriage in the nasopharynx
Encapsulated, kidney-bean shaped diplococcus
Neisseria
Natural host of Neisseria
Only human
Can form bactericidal and hemagglutinating antibodies
Neisseria
Most common cause among aged 2-18 years old
Meningitis
Clinical manifestation includes fever, headache, stiif neck and increased level of PMNs in CSF
Miningitis
Most sever form of meningococcemia with high fever, shock, widespread purpura, disseminated intravascular coagulation, thrombocytopenia and adrenal insufficiency and bilateral hemorrhagic destruction of the adrenal glands
Waterhouse-Friderichen Syndrome
Dissemination of meningococci into the bloodstream which causes from consumption coagulopathy into petechial or purpuric rash (purpura fulminans)
Mingococcemia
Positive in Meningococci but negative in gonococci
Polysaccharide capsule
Deficiencies in late acting complement components (C5-C9) predispose to illness that leads to cannot foem membrane attack complexes
Complement
Dark culture specimen on blood agar that has been heated to 80°C for 15 mins
Culture (Chocolate agar)
Prevents growth of bacteria using Thayer Martin Agar
Diagnosis of N meningitidis
Ferment both maltose and glucose
N. Meningitidis
Treatment of meningococcal meningitis and septicemia
Penicillin
Ceftriaxone/ cefotaxime
Drugs for Prophylaxis of close contacts of infected persons
Rifampin/ Ciproflaxin
Prevention for meningococcal which contains cqpsular polysaccharide of atrains A,C, Y, and W-135 coupled to a carrier protein (diptheria toxoid). The 1st meningococcal vaccine for serogroup B
Meningococcal polyvalent vaccine
A chronic dibilitated home care Px presents with fever, cough, sputum culture, revealed gram (-) motile rods with blue pigmented colonies. What is the causative organism?
Pseudomonas
A Px had diarrhea and eventially resolved, now he presents at the ER due to ascending paralysis which started 3 days ago. The physician diagnosed him eith Guillian-Barre syndrome. What is the most likely organism of the diarrhea?
Campylobacter
Most common anatomical site among carriers of N. miningitides.
Nasopharynx
Which of the following opportunistic bacteria is non-motile and gram (-) bacilli?
- Burkholderia
- Pseudomonas
- Acinetobacter
- Aeromonas
Acinetobacter
Which of the following opportunistic bacteria is non-motile and gram (-) bacilli?
- Burkholderia
- Pseudomonas
- Acinetobacter
- Aeromonas
Acinetobacter
The virulence factor of Gonococci that enhances its ability to penetrate the mucosal lining and able to preven immunity from the previous infection.
IgA proteases
Thr hypovolemic shock in Pxs that is attributed to severe dehydration due to the massive watery diarrhea in cholera is due to?
Enterotoxin
The hypovolemic shock in Pxs that is attributed to severe dehydration due to the massive watery diarrhea in cholera is due to?
Enterotoxin
True for gonococcal infection.
- Causes foul smelling and painful among females
- Discharge is greenish and mucoid
- Discharge is perfused and yellowish non-foul smelling
- it causes non-purulent, non painful discharge to males
Discharge is perfused and yellowish non-foul smelling
The presence of purpura fulminans is an indication of what sequelae caused by the organism’s endotoxin.
Consumptive coagulopathy
Primary affects the human genital tract and transmitted thru sexual and birth canal (vertical transmission)
Neisseria gonorrhoeae
Prevention of Miningitidis infection which contains capsular polysaccharide of strains A, C, Y, and W-135 coupled to a carrier protein (diphtheria toxoid) to enhance immunogenicity
MENINGOCOCCAL POLYVALENT VACCINE
To appreciate the morphology of Legionella pneumophila, which method should be used?
- cultured with Buffered Carchoal Extract Agar
- silver staining technique
- serologic test
- gram staining technique
silver staining technique
Drug/s of choice in treating N. miningitidis
Penicillin
Ceftriaxone (cefotaxime)
Culture used for N miningitidis and the selcecrive media.
Chocolate agar
Thayer Martin Agar
Inhibits gram (+) Colistin
Vancomycin
Inhibits gram (-) excrpt Neisseria
Trimethoprim
Inhibits fungi
Nystatin
Human host only with no immunity to repeated infections
N. gonorrhoeaa
Kidney bean-shaped with concave sides facing each other forming the appearance of doughnut Gram-negative diplococci.
N. gonorrhoeae
Most common sites of inoculation of N gonorrhoeae
- Cervix (cervicitis) or vagina in the female
* Urethra (urethritis) or penis in the male
A most common cause of urethritis
Gonococcal urethritis
Urethritis and epididymitis in men (“ Tulo” in Men)
A most common cause of urethritis
Gonococcal urethritis
Urethritis and epididymitis in men (“ Tulo” in Men)
A most common cause of urethritis
Gonococcal urethritis
Urethritis and epididymitis in men (“ Tulo” in Men)
the most common cause of septic arthritis in sexually active individuals
Gonococcal arthritis
N. gonorrhoeae is the most common, the most severe form of conjunctivitis.
Hyperactive bacterial conjunctivitis
purulent conjunctivitis in newborns
Ophthalmia neonaturum
Discharge is usually mucoid/mucopurulent and the only manifestation may be crusting in the morning
Non- gonococcal urethritis