mRNA Splicing and Translation Flashcards
What process generates different mRNAs from the same initial (primary) transcript
Alternate splicing
How can RNA Interference (RNAi) be used experimentally?
To knockdown protein production from a particular gene
Do all eukaryotic genes have introns?
No, not all eukaryotic genes have introns.
Can RNAi completely silence a gene?
No, RNAi reduces gene expression but does not necessarily completely silence a gene.
What are the typical sequence features of introns at their boundaries?
Introns typically begin with GU and end with AG
What is the purpose of alternate splicing in gene expression?
Alternative splicing allows the production of different protein isoforms, enabling changes in protein function
Are all tRNAs capped at the 5’ end?
No, not all tRNAs are capped at the 5’ end
What is the significance of intron splicing in eukaryotic genes?
SPlicing removes introns from pre-mRNA, ensuring proper assembly of exons into mature mRNA for translation
What are proteins synthesised by?
The success addition of amino acids at the carboxy terminus.
Generating genetic mutations at a desired location in the genome is an experimental use of which system?
CRISPR-Cas
How is the CRISPR-Cas complex targeted to specific regions of the genome?
With a guide RNA that is complementary to the target DNA sequence
What transcribes genes encoding aminoacyl tRNA synthases?
RNA polymerase II
