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CLIN CHEM LAB > MOVING EXAM > Flashcards

MOVING EXAM Flashcards

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1
Q

is a major carbohydrate present in the blood & serves as a primary source
of energy

A

GLUCOSE

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2
Q

Glucose is usually obtained from ingested ________ and ________

A

starch and sugar

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3
Q

Excessive glucose is stored
as inactive glycogen mainly in the ________ and little in the ___________

A

liver and little in the muscle

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4
Q

Elevated blood glucose levels are found in _________, ________, ________, __________

A

diabetes mellitus, hyperthyroidism,
hyperadrenalism & certain liver diseases

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5
Q

Decreased levels are found in _________, _______, _________

A

Insulinoma, hypothyroidism, hypopituitarism.

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6
Q

Enzymatic __________determination of glucose according to the following
reaction.

A

colorimetric determination

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7
Q

This reagent is intended for in vitro quantitative determination of Glucose in _______, ______, _______

A

serum,
plasma & CSF.

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8
Q

This reagent is intended for in vitro quantitative determination of Albumin in ______ or _______

A

serum or plasma

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9
Q

GOD-PAP methodology meaning and under by what

A

Glucose oxidase-phenol amino phenazone
Glucose

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10
Q
  • Linear upto _______ mg/dL - Glucose
A

600 mg/dl

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11
Q

Material Safety data sheets (MSDS) will be provided on request

A

Risk & safety

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12
Q

Glucose Reagent & Standard are ready to use.

A

Reagent Preparation

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13
Q

The sealed reagents are stable up to the expiry date stated on the label, when
stored at 2- 8o
C and protected from light

A

Reagent Storage and Stability

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14
Q

Once opened, the reagent is stable up to 4 weeks at 2-80
C,if contamination is
avoided

A

Open Vial Stability

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15
Q

On-board Calibration stability is 20 days

A

Onboard Calibration Stability

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16
Q

Turbidity or precipitation in any kit component indicates deterioration and the
component must be discarded. Values outside the recommended acceptable range
for the Agappe Qualicheck Norm & Path control may also be an indication of
reagent instability and associated results are invalid. Sample should be retested
using a fresh vial of reagent.

A

Reagent Deterioration

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17
Q

To avoid contamination, use clean laboratory wares use clean, drydisposable pipette
tips for dispensing. Close reagent bottles immediately after use

A

Precaution

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18
Q

Avoid direct exposure of reagent to light. Do not blow into the reagent bottles.

A

Precaution

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19
Q

Reagents must be disposed off in accordance with local regulations

A

Waste Management

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20
Q

Serum / plasma (free of hemolysis) / CSF

A

Sample

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21
Q

Glucose reagent & Standard

A

Materials provided

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22
Q

Pipettes & Tips
Test Tubes & racks
Timer
Incubator
Analyzer

A

Materials required but not provided

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23
Q

Mode of Reaction

A

End point

24
Q

Slope of reaction

A

Increasing

25
Q

Wavelength

A

505 (490-550 nm)

26
Q

Temperature

A

37 degrees C

27
Q

Standard Concentration

A

100 mg/dL

28
Q

Linearity

A

600 mg dl

29
Q

Incubation Time

A

10 Minutes

30
Q

Blank

A

Reagent

31
Q

Sample volume

A

10 µL

32
Q

Reagent volume

A

1000 µL

33
Q

Cuvette

A

1 cm light path

34
Q

Traditional Unit - Glucose

A

mg/dl

35
Q

SI Unit glucose

A

mmol/L

36
Q

Conversion from Traditional to SI glucose

A

0.05551

37
Q

___________ is recommended for Calibration of this assay in fully auto analyzers. glucose

A

Agappe multicalibrator

38
Q

Provided standard is recommended for calibration of this assay on ___________ glucose

A

semi auto analyzer

39
Q

Mix & incubate for ______ minutes at _______ Read the absorbance of standard and
sample against reagent blank.

A

10 minutes at 37 degrees celcius

40
Q

Glucose Reagent of BCS in glucose

A

1000 uL

41
Q

Standard BCS glucose

A

10 uL

42
Q

Sample / control - glucose

A

10 uL

43
Q

Calculation of glucose

A

Glucose Concentration (mg/dL) = Absorbance of sample/ Absorbance of standard X standard concentration

44
Q

It is recommended to use Agappe Qualicheck Norm & Path ( 11601001) to verify
the performance of the assay. Each laboratory has to establish its own internal
quality control scheme and procedure for corrective action, if control do not recover
within the acceptable range.

A

Quality control

45
Q

If the concentration is greater than linearity (600 mg/dL)dilute the sample with
normal saline and repeat the assay. Multiply the result with dilution factor

A
  1. Linearity
46
Q

A comparison study has been performed between Agappe reagent and another
internationally available reagent yielded a correlation coefficient of r2= 0.9989
and a regression equation of y = 1.0016x.

A
  1. Comparison
47
Q

Lower detection Limit is 1.0 mg/dL

A
  1. Sensitivity
48
Q

Give me the 4 performance

A
  1. linearity
  2. Comparison
  3. Precision
  4. Sensitivity
49
Q

It is recommended that each laboratory establish its own reference values.
The following value may be used as guide line

A

Reference Range

50
Q

_________ which is synthesized in the liver constitutes a major part of the total proteins in the body, the other part being globulin, they form the major portion of the dissolved substances in the plasma. Functions of al bumin includes distribution of extracellular fluid, regulation of osmotic pressure, acts as transport agent for wide variety of substance such as hormones, lipids, vitamins etc. Increased levels are seen in dehydration. Decreased levels are seen in liver disease (Hepatitis. Cirrhosis), malnutrition, kidney disorders, and increased fluid loss during extensive burns & malabsorption.

A

Albumin

51
Q

Methodology use in Albumin

A

Bromocresol Green methodology

52
Q

Linear of albumin

A

6 g/dl

53
Q

The reaction between albumin from serum or plasma and the dye bromocresol green produces a change in colour that is proportional to the albumin concentration

A

Albumin

54
Q

____________ is recommended for calibration of this assay on semi auto analyzers.

A

Albumin standard

55
Q

Direct Biuret Method
Linear up to 15 g/dL

A

TOTAL PROTEIN

CLIN CHEM LAB (14 decks)

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