MOVING EXAM

Question 1

is a major carbohydrate present in the blood & serves as a primary source
of energy

Answer 1

GLUCOSE

Question 2

Glucose is usually obtained from ingested ________ and ________

Answer 2

starch and sugar

Question 3

Excessive glucose is stored
as inactive glycogen mainly in the ________ and little in the ___________

Answer 3

liver and little in the muscle

Question 4

Elevated blood glucose levels are found in _________, ________, ________, __________

Answer 4

diabetes mellitus, hyperthyroidism,
hyperadrenalism & certain liver diseases

Question 5

Decreased levels are found in _________, _______, _________

Answer 5

Insulinoma, hypothyroidism, hypopituitarism.

Question 6

Enzymatic __________determination of glucose according to the following
reaction.

Answer 6

colorimetric determination

Question 7

This reagent is intended for in vitro quantitative determination of Glucose in _______, ______, _______

Answer 7

serum,
plasma & CSF.

Question 8

This reagent is intended for in vitro quantitative determination of Albumin in ______ or _______

Answer 8

serum or plasma

Question 9

GOD-PAP methodology meaning and under by what

Answer 9

Glucose oxidase-phenol amino phenazone
Glucose

Question 10

• Linear upto _______ mg/dL - Glucose

Answer 10

600 mg/dl

Question 11

Material Safety data sheets (MSDS) will be provided on request

Answer 11

Risk & safety

Question 12

Glucose Reagent & Standard are ready to use.

Answer 12

Reagent Preparation

Question 13

The sealed reagents are stable up to the expiry date stated on the label, when
stored at 2- 8o
C and protected from light

Answer 13

Reagent Storage and Stability

Question 14

Once opened, the reagent is stable up to 4 weeks at 2-80
C,if contamination is
avoided

Answer 14

Open Vial Stability

Question 15

On-board Calibration stability is 20 days

Answer 15

Onboard Calibration Stability

Question 16

Turbidity or precipitation in any kit component indicates deterioration and the
component must be discarded. Values outside the recommended acceptable range
for the Agappe Qualicheck Norm & Path control may also be an indication of
reagent instability and associated results are invalid. Sample should be retested
using a fresh vial of reagent.

Answer 16

Reagent Deterioration

Question 17

To avoid contamination, use clean laboratory wares use clean, drydisposable pipette
tips for dispensing. Close reagent bottles immediately after use

Answer 17

Precaution

Question 18

Avoid direct exposure of reagent to light. Do not blow into the reagent bottles.

Answer 18

Precaution

Question 19

Reagents must be disposed off in accordance with local regulations

Answer 19

Waste Management

Question 20

Serum / plasma (free of hemolysis) / CSF

Answer 20

Sample

Question 21

Glucose reagent & Standard

Answer 21

Materials provided

Question 22

Pipettes & Tips
Test Tubes & racks
Timer
Incubator
Analyzer

Answer 22

Materials required but not provided

Question 23

Mode of Reaction

Answer 23

End point

Question 24

Slope of reaction

Answer 24

Increasing

Question 25

Wavelength

Answer 25

505 (490-550 nm)

Question 26

Temperature

Answer 26

37 degrees C

Question 27

Standard Concentration

Answer 27

100 mg/dL

Question 28

Linearity

Answer 28

600 mg dl

Question 29

Incubation Time

Answer 29

10 Minutes

Question 30

Blank

Answer 30

Reagent

Question 31

Sample volume

Answer 31

10 µL

Question 32

Reagent volume

Answer 32

1000 µL

Question 33

Cuvette

Answer 33

1 cm light path

Question 34

Traditional Unit - Glucose

Answer 34

mg/dl

Question 35

SI Unit glucose

Answer 35

mmol/L

Question 36

Conversion from Traditional to SI glucose

Answer 36

0.05551

Question 37

___________ is recommended for Calibration of this assay in fully auto analyzers. glucose

Answer 37

Agappe multicalibrator

Question 38

Provided standard is recommended for calibration of this assay on ___________ glucose

Answer 38

semi auto analyzer

Question 39

Mix & incubate for ______ minutes at _______ Read the absorbance of standard and sample against reagent blank.

Answer 39

10 minutes at 37 degrees celcius

Question 40

Glucose Reagent of BCS in glucose

Answer 40

1000 uL

Question 41

Standard BCS glucose

Answer 41

10 uL

Question 42

Sample / control - glucose

Answer 42

10 uL

Question 43

Calculation of glucose

Answer 43

Glucose Concentration (mg/dL) = Absorbance of sample/ Absorbance of standard X standard concentration

Question 44

It is recommended to use Agappe Qualicheck Norm & Path ( 11601001) to verify the performance of the assay. Each laboratory has to establish its own internal quality control scheme and procedure for corrective action, if control do not recover within the acceptable range.

Answer 44

Quality control

Question 45

If the concentration is greater than linearity (600 mg/dL)dilute the sample with normal saline and repeat the assay. Multiply the result with dilution factor

Answer 45

1. Linearity

Question 46

A comparison study has been performed between Agappe reagent and another internationally available reagent yielded a correlation coefficient of r2= 0.9989 and a regression equation of y = 1.0016x.

Answer 46

2. Comparison

Question 47

Lower detection Limit is 1.0 mg/dL

Answer 47

4. Sensitivity

Question 48

Give me the 4 performance

Answer 48

1. linearity 2. Comparison 3. Precision 4. Sensitivity

Question 49

It is recommended that each laboratory establish its own reference values. The following value may be used as guide line

Answer 49

Reference Range

Question 50

_________ which is synthesized in the liver constitutes a major part of the total proteins in the body, the other part being globulin, they form the major portion of the dissolved substances in the plasma. Functions of al bumin includes distribution of extracellular fluid, regulation of osmotic pressure, acts as transport agent for wide variety of substance such as hormones, lipids, vitamins etc. Increased levels are seen in dehydration. Decreased levels are seen in liver disease (Hepatitis. Cirrhosis), malnutrition, kidney disorders, and increased fluid loss during extensive burns & malabsorption.

Answer 50

Albumin

Question 51

Methodology use in Albumin

Answer 51

Bromocresol Green methodology

Question 52

Linear of albumin

Answer 52

6 g/dl

Question 53

The reaction between albumin from serum or plasma and the dye bromocresol green produces a change in colour that is proportional to the albumin concentration

Answer 53

Albumin

Question 54

____________ is recommended for calibration of this assay on semi auto analyzers.

Answer 54

Albumin standard

Question 55

Direct Biuret Method Linear up to 15 g/dL

Answer 55

TOTAL PROTEIN