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CLIN CHEM LAB > AGAPPE > Flashcards

AGAPPE Flashcards

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1
Q

GOD-PAP meaning

A

Glucose oxidase-phenol amino phenazone.

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2
Q

Methodology use in glucose?

A

GOD PAP

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3
Q

Linearity of glucose

A

600 mg/dl

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4
Q

___________ is a major carbohydrate present in the blood & serves as a primary source

A

Glucose

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5
Q

Glucose is usually obtained from ingested_______ and ________

A

Starch and sugar

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6
Q

Excessive glucose is stored
as inactive glycogen mainly in the _______ and ________

A

liver & little in the muscles.

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7
Q

Elevated blood glucose levels are found in _________, _______, _________

A

diabetes mellitus, hyperthyroidism,
hyperadrenalism & certain liver diseases.

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8
Q

Decreased levels are found in ________, _______, _______

A

Insulinoma, hypothyroidism, hypopituitarism

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9
Q

Quinonimine color

A

Red

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10
Q

Glucose color kay _________

A

Red

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11
Q

Product code of 11406001 of Glucose

A

5 X 100 mL

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12
Q

Product code of 1146002 of glucose

A

1 X 1000 mL

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13
Q

product of glucose 1146001 in glucose standard

A

1 X 4 mL

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14
Q

product of glucose 1146002 in glucose reagent

A

1 X mL

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15
Q

Description of glucose reagent 1

A

Tris Buffer (pH 7.40) -
92 mmol/L

Phenol - 0.3 mmol/L

Glucose Oxidase - 15000 U/L

4- Aminophenazone -
2.6 mmol/L

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16
Q

Description of glucose standard

A

glucose standard concentration 100 mg/dl

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17
Q

Glucose Reagent & Standard are ready to use.

A

Reagent Preparation

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18
Q

The sealed reagents are stable up to the expiry date stated on the label, when
stored at 2- 8o
C and protected from light.

A

Reagent Storage and Stability

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19
Q

Once opened, the reagent is stable up to 4 weeks at 2-80
C,if contamination is
avoided.

A

Open Vial Stability

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20
Q

On board calibration of Glucose

A

20 days

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21
Q

Turbidity or precipitation in any kit component indicates deterioration and the
component must be discarded. Values outside the recommended acceptable range
for the Agappe Qualicheck Norm & Path control may also be an indication of
reagent instability and associated results are invalid. Sample should be retested
using a fresh vial of reagent.

A

Reagent Deterioration

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22
Q

To avoid contamination, use clean laboratory wares use clean, drydisposable pipette
tips for dispensing. Close reagent bottles immediately after use.
Avoid direct exposure of reagent to light. Do not blow into the reagent bottles.
This reagent is only for IVD use and follow the normal precautions required for
handling all laboratory reagents

A

Precaution

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23
Q

Reagents must be disposed off in accordance with local regulations

A

waste management

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24
Q

Serum / plasma (free of hemolysis) / CSF

A

Sample

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25
Q

Give me the interferences of glucose

A

Bilirubin up to 20 mg/dL
Haemoglobin up to 1000 mg/dL

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26
Q

Materials provided

A

Glucose reagent & Standard

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27
Q

Glucose

Mode of reaction
Slope of reaction
Wavelength
Temperature
Standard concentration
Linearity
Incubation time
Blank
Sample volume
Reagent volume
Cuvette

A

End point
Increasing
505
37 degrees celcius
100
600
10 minutes
reagent
10 uL
1000 uL
1 cm light path

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28
Q

Procedure notes for glucose
Glucose reagent
Standard
Sample/Control

A

Blank Calibrator and Sample
- 1000 uL
Standard - 10 uL
Sample control - 10 uL

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29
Q

Calculation of glucose

A

Glucose Concentration (mg/dL) = absorbance of sample/ absorbance of standard X standard concentration

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30
Q

It is recommended to use Agappe Qualicheck Norm & Path ( 11601001) to verify
the performance of the assay. Each laboratory has to establish its own internal
quality control scheme and procedure for corrective action, if control do not recover
within the acceptable range

A

Quality control

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31
Q

It is recommended that each laboratory establish its own reference values.
The following value may be used as guide line

A

Reference range

32
Q

This reagent is linear upto 600 mg/dL.
If the concentration is greater than linearity (600 mg/dL)dilute the sample with
normal saline and repeat the assay. Multiply the result with dilution factor.

A

Linearity

33
Q

A comparison study has been performed between Agappe reagent and another
internationally available reagent yielded a correlation coefficient of r2= 0.9989
and a regression equation of y = 1.0016x

A
  1. Comparison
34
Q

Reference range of glucose
: 1 day :
: >1 day
: Child :
: Adult :
: >60 Years :
:>90 Years :
WB(Hep) : Adult
CSF : Infant child :
: Adult :
Urine :
U-24 hrs :

A

: 1 day :40-60 mg/dL
: >1 day :50-80 mg/dL
: Child :60-100 mg/dL
: Adult :74-100 mg/dL
: >60 Years :82-115 mg/dL
:>90 Years :75-121 mg/dL
WB(Hep) : Adult :65-95 mg/dL
CSF : Infant child :60-80 mg/dL
: Adult :40-70 mg/dL
Urine : 1-15 mg/dL
U-24 hrs : <0.5 gram/day

35
Q

Glucose Sensitivity

A

Lower detection Limit is 1.0 mg/dL

36
Q

Total protein methodology

A

Direct biuret method

37
Q

Total protein linearity

A

15 g/dl

38
Q

__________ form the major portion of dissolved substances in the plasma. They form the basic structural components of the body. They constitute the enzymes present in our body also act as secondary source of energy.

A

Proteins

39
Q

functions of total proteins

A

The other functions include distribution of water, buffering, transport of various components, defense & coagulation of blood in our body.

40
Q

Increased level of total protein

A

dehydration & myeloma.

41
Q

decrease level of glucose are found in

A

Decreased levels are found in liver disorders, Nephrotic syndrome, malnutrition & protein due to haemorrhage.

42
Q

Product code of Total protein reagent

A

4 X 50 mL

43
Q

Product code of total protein standard

A

1 X 3 mL

44
Q

Description of total protein reagent

A

Potassium Iodide 6 mmol/L
Potassium sodium tartarate 21 mmol/L
Copper Sulphate 6 mmol/L
Sodium Hydroxide 58 mmol/L

45
Q

Glucose

Open Vial Stability
Once opened, the reagent is stable up to _____ weeks at _______
C,if contamination is
avoided.

A

4 weeks - 2-8 degrees celcius

46
Q

Total protein

Open vial stability
Once opened, the reagent is stable up to ______ days if contamination is avoided.

A

20

47
Q

Total protein
On board calibration

On board calibration stability is ______

A

7 days

48
Q

Interferences of total protein

A

No interference for
Ascorbic Acid up to
500 mg/dL
Hemoglobin up to 1000 mg/dL
Bilirubin up to
20 mg/dL

49
Q

Total protein

Mode of reaction
Slope of reaction
Wavelength
Temperature
Standard concentration
Linearity
Incubation time
Blank
Sample volume
Reagent volume
Cuvette

A

end point
increasing
546
37 degrees celcius
6 g/dl
15 g/dl
10 minutes
reagent
20 uL
1000 uL
1 cm light path

50
Q

Traditional unit
SI unit
conversion from traditional to SI

A

g/dl
g/L
X 10

51
Q

Procedure notes of total protein

Reagent
Standard
Sample

A

1000 uL
1000 uL
1000 uL

20 uL - standard
20 uL - sample

51
Q

Procedure notes of total protein

Reagent
Standard
Sample

A

1000 uL
1000 uL
1000 uL

20 uL - standard
20 uL - sample

52
Q

Total protein

A

Total Protein Con. (g/dL) Absorbance of Sample Absorbance of Standard X6

53
Q

The procedure is linear upto 15 g/dL If the concentration is greater than linearity (15 g/dL), dilute the sample with normal saline and repeat the assay. Multiply the result with dilution factor.

A

Linearity

54
Q

A comparison study has been performed between Agappe reagent and another internationally available reagent yielded a correlation coefficient of r -0.9586 and a regression equation of y = 0.9808x.

A

Comparison

55
Q

Sensitivity of total protein

A

Lower detection Limit is 0.2 g/dL
Bibliography

56
Q

Color of total protein

A

Purple

57
Q

Color of albumin

A

Green

58
Q

Methodology use for Albumin

A

Bromocresol green methodology

59
Q

Linearity of albumin

A

6 g/dl

60
Q

___________ which is synthesized in the liver constitutes a major part of the total proteins in the body, the other part being globulin, they form the major portion d the dissolved substances in the plasma.

A

Albumin

61
Q

Function of albumin
increase and decrease

A

Functions of albumin includes distribution of extracellular fluid, regulation of osmotic pressure, acts as transport agent for a wide variety of substance such as hormones, lipids, vitamins etc. Increased level are seen in dehydration. Decreased levels are seen in liver disease (Hepatitis Cirrhosis), malnutrition, kidney disorders, and increased fluid loss during extensive burns & malabsorption.

62
Q

Description of Albumin reagent

A

succinate buffer (ph 4.20) 75 mmol / L
Bromocresol green 0.14

63
Q

Albumin standard description

A

albumin standard concentration 3 g/dl

64
Q

On board calibration stability in albumin

A

10 days

65
Q

open vial stability in albumin

A

20 days

66
Q

Interferences of albumin

A

Ascorbic acid - 50 mg/dl
hemoglobin - 1000 mg/dl
bilirubin - 20 mg/dl

67
Q

Albumin

Mode of reaction
Slope of reaction
Wavelength
Temperature
Standard concentration
Linearity
Incubation time
Blank
Sample volume
Reagent volume
Cuvette

A

end point
increasing
630 nm
30 degrees celcius
3 g/dl
6 g/dl
1 minute
reagent
10 ul
1000 ul
1 cm light path

68
Q

ALBUMIN

traditional unit
SI unit
Conversion from traditional unit

A

g/dl
g/l
X10

69
Q

Albumin

Reagent
standard
sample

A

1000 ul
10 ul
10 ul

70
Q

Albumin calculation

A

albumin concentration = absorbance of sample/ absorbance of reagent X 3

71
Q

Reference range of albumin

A

Serum/Plasma: 0-4 days
4 days-14 years
:2.8-4.4 g/dL
:3.8-5.4 g/dL
14-18 Years
:3.2-4.5 g/dL
Adult (20-60 years)
:3.5-5.2 g/dL
60-90 Years
:3.2-4.6 g/dL
>90 Years
:2.9-4.5 g/dL

72
Q

This reagent is linear upto 6 g/dL
If the concentration is greater than linearity (6 g/dL), dilute the sample with normal saline and repeat the assay. Multiply the result with dilution factor.

A

Linearity

73
Q

A comparison study has been performed between Agappe reagent and another internationally available reagent yielded a correlation coefficient of r = 0.955 and a regression equation of y = 1.0034x.

A

Comparison

74
Q

Sensitivity of Albumin

A

Lower detection Limit is 0.1 g/dL

CLIN CHEM LAB (14 decks)

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