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AGAPPE Flashcards

1
Q

GOD-PAP meaning

A

Glucose oxidase-phenol amino phenazone.

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2
Q

Methodology use in glucose?

A

GOD PAP

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3
Q

Linearity of glucose

A

600 mg/dl

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4
Q

___________ is a major carbohydrate present in the blood & serves as a primary source

A

Glucose

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5
Q

Glucose is usually obtained from ingested_______ and ________

A

Starch and sugar

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6
Q

Excessive glucose is stored
as inactive glycogen mainly in the _______ and ________

A

liver & little in the muscles.

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7
Q

Elevated blood glucose levels are found in _________, _______, _________

A

diabetes mellitus, hyperthyroidism,
hyperadrenalism & certain liver diseases.

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8
Q

Decreased levels are found in ________, _______, _______

A

Insulinoma, hypothyroidism, hypopituitarism

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9
Q

Quinonimine color

A

Red

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10
Q

Glucose color kay _________

A

Red

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11
Q

Product code of 11406001 of Glucose

A

5 X 100 mL

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12
Q

Product code of 1146002 of glucose

A

1 X 1000 mL

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13
Q

product of glucose 1146001 in glucose standard

A

1 X 4 mL

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14
Q

product of glucose 1146002 in glucose reagent

A

1 X mL

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15
Q

Description of glucose reagent 1

A

Tris Buffer (pH 7.40) -
92 mmol/L

Phenol - 0.3 mmol/L

Glucose Oxidase - 15000 U/L

4- Aminophenazone -
2.6 mmol/L

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16
Q

Description of glucose standard

A

glucose standard concentration 100 mg/dl

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17
Q

Glucose Reagent & Standard are ready to use.

A

Reagent Preparation

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18
Q

The sealed reagents are stable up to the expiry date stated on the label, when
stored at 2- 8o
C and protected from light.

A

Reagent Storage and Stability

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19
Q

Once opened, the reagent is stable up to 4 weeks at 2-80
C,if contamination is
avoided.

A

Open Vial Stability

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20
Q

On board calibration of Glucose

A

20 days

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21
Q

Turbidity or precipitation in any kit component indicates deterioration and the
component must be discarded. Values outside the recommended acceptable range
for the Agappe Qualicheck Norm & Path control may also be an indication of
reagent instability and associated results are invalid. Sample should be retested
using a fresh vial of reagent.

A

Reagent Deterioration

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22
Q

To avoid contamination, use clean laboratory wares use clean, drydisposable pipette
tips for dispensing. Close reagent bottles immediately after use.
Avoid direct exposure of reagent to light. Do not blow into the reagent bottles.
This reagent is only for IVD use and follow the normal precautions required for
handling all laboratory reagents

A

Precaution

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23
Q

Reagents must be disposed off in accordance with local regulations

A

waste management

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24
Q

Serum / plasma (free of hemolysis) / CSF

A

Sample

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25
Give me the interferences of glucose
Bilirubin up to 20 mg/dL Haemoglobin up to 1000 mg/dL
26
Materials provided
Glucose reagent & Standard
27
Glucose Mode of reaction Slope of reaction Wavelength Temperature Standard concentration Linearity Incubation time Blank Sample volume Reagent volume Cuvette
End point Increasing 505 37 degrees celcius 100 600 10 minutes reagent 10 uL 1000 uL 1 cm light path
28
Procedure notes for glucose Glucose reagent Standard Sample/Control
Blank Calibrator and Sample - 1000 uL Standard - 10 uL Sample control - 10 uL
29
Calculation of glucose
Glucose Concentration (mg/dL) = absorbance of sample/ absorbance of standard X standard concentration
30
It is recommended to use Agappe Qualicheck Norm & Path ( 11601001) to verify the performance of the assay. Each laboratory has to establish its own internal quality control scheme and procedure for corrective action, if control do not recover within the acceptable range
Quality control
31
It is recommended that each laboratory establish its own reference values. The following value may be used as guide line
Reference range
32
This reagent is linear upto 600 mg/dL. If the concentration is greater than linearity (600 mg/dL)dilute the sample with normal saline and repeat the assay. Multiply the result with dilution factor.
Linearity
33
A comparison study has been performed between Agappe reagent and another internationally available reagent yielded a correlation coefficient of r2= 0.9989 and a regression equation of y = 1.0016x
2. Comparison
34
Reference range of glucose : 1 day : : >1 day : Child : : Adult : : >60 Years : :>90 Years : WB(Hep) : Adult CSF : Infant child : : Adult : Urine : U-24 hrs :
: 1 day :40-60 mg/dL : >1 day :50-80 mg/dL : Child :60-100 mg/dL : Adult :74-100 mg/dL : >60 Years :82-115 mg/dL :>90 Years :75-121 mg/dL WB(Hep) : Adult :65-95 mg/dL CSF : Infant child :60-80 mg/dL : Adult :40-70 mg/dL Urine : 1-15 mg/dL U-24 hrs : <0.5 gram/day
35
Glucose Sensitivity
Lower detection Limit is 1.0 mg/dL
36
Total protein methodology
Direct biuret method
37
Total protein linearity
15 g/dl
38
__________ form the major portion of dissolved substances in the plasma. They form the basic structural components of the body. They constitute the enzymes present in our body also act as secondary source of energy.
Proteins
39
functions of total proteins
The other functions include distribution of water, buffering, transport of various components, defense & coagulation of blood in our body.
40
Increased level of total protein
dehydration & myeloma.
41
decrease level of glucose are found in
Decreased levels are found in liver disorders, Nephrotic syndrome, malnutrition & protein due to haemorrhage.
42
Product code of Total protein reagent
4 X 50 mL
43
Product code of total protein standard
1 X 3 mL
44
Description of total protein reagent
Potassium Iodide 6 mmol/L Potassium sodium tartarate 21 mmol/L Copper Sulphate 6 mmol/L Sodium Hydroxide 58 mmol/L
45
Glucose Open Vial Stability Once opened, the reagent is stable up to _____ weeks at _______ C,if contamination is avoided.
4 weeks - 2-8 degrees celcius
46
Total protein Open vial stability Once opened, the reagent is stable up to ______ days if contamination is avoided.
20
47
Total protein On board calibration On board calibration stability is ______
7 days
48
Interferences of total protein
No interference for Ascorbic Acid up to 500 mg/dL Hemoglobin up to 1000 mg/dL Bilirubin up to 20 mg/dL
49
Total protein Mode of reaction Slope of reaction Wavelength Temperature Standard concentration Linearity Incubation time Blank Sample volume Reagent volume Cuvette
end point increasing 546 37 degrees celcius 6 g/dl 15 g/dl 10 minutes reagent 20 uL 1000 uL 1 cm light path
50
Traditional unit SI unit conversion from traditional to SI
g/dl g/L X 10
51
Procedure notes of total protein Reagent Standard Sample
1000 uL 1000 uL 1000 uL 20 uL - standard 20 uL - sample
51
Procedure notes of total protein Reagent Standard Sample
1000 uL 1000 uL 1000 uL 20 uL - standard 20 uL - sample
52
Total protein
Total Protein Con. (g/dL) Absorbance of Sample Absorbance of Standard X6
53
The procedure is linear upto 15 g/dL If the concentration is greater than linearity (15 g/dL), dilute the sample with normal saline and repeat the assay. Multiply the result with dilution factor.
Linearity
54
A comparison study has been performed between Agappe reagent and another internationally available reagent yielded a correlation coefficient of r -0.9586 and a regression equation of y = 0.9808x.
Comparison
55
Sensitivity of total protein
Lower detection Limit is 0.2 g/dL Bibliography
56
Color of total protein
Purple
57
Color of albumin
Green
58
Methodology use for Albumin
Bromocresol green methodology
59
Linearity of albumin
6 g/dl
60
___________ which is synthesized in the liver constitutes a major part of the total proteins in the body, the other part being globulin, they form the major portion d the dissolved substances in the plasma.
Albumin
61
Function of albumin increase and decrease
Functions of albumin includes distribution of extracellular fluid, regulation of osmotic pressure, acts as transport agent for a wide variety of substance such as hormones, lipids, vitamins etc. Increased level are seen in dehydration. Decreased levels are seen in liver disease (Hepatitis Cirrhosis), malnutrition, kidney disorders, and increased fluid loss during extensive burns & malabsorption.
62
Description of Albumin reagent
succinate buffer (ph 4.20) 75 mmol / L Bromocresol green 0.14
63
Albumin standard description
albumin standard concentration 3 g/dl
64
On board calibration stability in albumin
10 days
65
open vial stability in albumin
20 days
66
Interferences of albumin
Ascorbic acid - 50 mg/dl hemoglobin - 1000 mg/dl bilirubin - 20 mg/dl
67
Albumin Mode of reaction Slope of reaction Wavelength Temperature Standard concentration Linearity Incubation time Blank Sample volume Reagent volume Cuvette
end point increasing 630 nm 30 degrees celcius 3 g/dl 6 g/dl 1 minute reagent 10 ul 1000 ul 1 cm light path
68
ALBUMIN traditional unit SI unit Conversion from traditional unit
g/dl g/l X10
69
Albumin Reagent standard sample
1000 ul 10 ul 10 ul
70
Albumin calculation
albumin concentration = absorbance of sample/ absorbance of reagent X 3
71
Reference range of albumin
Serum/Plasma: 0-4 days 4 days-14 years :2.8-4.4 g/dL :3.8-5.4 g/dL 14-18 Years :3.2-4.5 g/dL Adult (20-60 years) :3.5-5.2 g/dL 60-90 Years :3.2-4.6 g/dL >90 Years :2.9-4.5 g/dL
72
This reagent is linear upto 6 g/dL If the concentration is greater than linearity (6 g/dL), dilute the sample with normal saline and repeat the assay. Multiply the result with dilution factor.
Linearity
73
A comparison study has been performed between Agappe reagent and another internationally available reagent yielded a correlation coefficient of r = 0.955 and a regression equation of y = 1.0034x.
Comparison
74
Sensitivity of Albumin
Lower detection Limit is 0.1 g/dL

CLIN CHEM LAB (14 decks)

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