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CLIN CHEM LAB > AGAPPE FINALS > Flashcards

AGAPPE FINALS Flashcards

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1
Q

HDL cholesterol is linear up to _______?

A

125 mg /dl

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2
Q

Method of HDL use

A

Precipitation method

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3
Q

___________ are the proteins, which mainly transport lipids in the blood stream.

A

Lipoproteins

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4
Q

LDL carries cholesterol to the peripheral tissues where it can be deposited and increase the risk of ____________and ___________

A

atherosclerotic heart and peripheral vascular disease

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5
Q

Hence high levels of HDl are ___________

A

atherogenic

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6
Q

serum HDL helps identify patients a t risk developing _________

A

coronary heart disease

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7
Q

The chylomicrons, VLDL, LDL are precipitated by ________

A

phosphotungstic acid and magnesium ions

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8
Q

After centrifugation high density are in the _______

A

supernatant

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9
Q

HDL content of supernatant is measured by an __________

A

enzymatic method

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10
Q

description of HDL cholesterol reagent

A

phosphotungstic 14 mmol
magnesium chloride 1 mmol
preservatives

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11
Q

description of HDL cholesterol standard

A

HDL cholesterol standard concentration 50 mg/dl

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12
Q

product code of HDL cholesterol reagent

A

4 X 25

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13
Q

product of HDL cholesterol standard

A

1 X 4

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14
Q

interferences of HDL

A

bilirubin - 10 mg/dl
hemoglobin - 1000 mg/dl

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15
Q

materials provided in hdl cholesterol

A

hdl cholesterol standard and hdl cholesterol reagent

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16
Q

materials required but not provided
(6)

A

Cholesterol reagent
Pipettes and tips
Test tubes and racks
Timer
Incubator
Analyzer

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17
Q

HDL

Test of parameters
Mode of reaction
Slope of reaction
Wavelength
Wavelength
Temperature
Standard concentration
Blank
Linearity
Incubation time
Sample volume
reagent volume
cuvette

A

endpoint
increasing
505 (500-550 nm)
630 nm
37 C
125 mg/dl
Cholesterol reagent
125 mg /dl
5 minutes
50 ul
1000 ul
1 cm light path

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18
Q

traditional unit
SI unit
Conversion from Tradition to SI

A

mg/dl
mmol/L
X0.026

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19
Q

I. precipitation
sample for HDL
HDL reagent

A

300 uL
300 uL

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20
Q

HDL
Mix well, allow to stand for 10 min at room temperature, mix again and centrifuge for 10 min, at _____________

A
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21
Q

HDL
Mix well, allow to stand for _________at room temperature, mix again and centrifuge for __________, at _____________

A

10 min/10 min/ at 4000rpm

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22
Q

HDL cholesterol determination

cholesterol reagent
HDL standard
HDL supernatant

A

1000 uL
1000 uL
1000 uL
50 uL
50 uL

23
Q

HDL cholesterol and determination
Mix well and incubate for ________ at ________ measure the absorbance of standard and sample against the reagent bottle

A

5 minutes at 37 C

24
Q

Calculation of HDL cholesterol

A

HDL cholesterol conc (mg/dl) = absorbance of sample
/ absorbance of standard X concentration of standard X 2

25
Q

3 performance of HDL

A
  1. linearity
  2. Precision
  3. Sensitivity
26
Q

It is recommended to establish its own reference values

A

Reference range

27
Q

HDL cholesterol of men and women

A

35-55 mg/dl
45-55 mg/dl

28
Q

LDL cholesterol of men and women

A

suspicious 150 mg/dl
elevated 190 mg/dl

29
Q

HDL reagent is linear up to ____________

A

125 mg/dl

30
Q

Precision HDL

qualicheck norm under expected value and measured value
qualicheck path under expected and measured value

A

30 +- 4.5
32
70 +- 40
68.51

31
Q

HDL lower sensitivity limit is _________

A

1.0 mg/dl

32
Q

Triglycerides methodology use

A

GPO TOPS
Glucose phosphate oxidase

33
Q

Triglycerides is linear up to __________

A

1000 mL

34
Q

Contains LCF which minimizes rerun

A

Triglycerides

35
Q

__________ are simple lipids, formed in the liver by _______ and ________

A

Triglycerides
glycerol and fatty acids

36
Q

Triglycerides transported by VLDL, LDL, and constitute about _____ of fat, stored as source of energy in the _____ and _________

A

95% —— tissue and plasma

37
Q

Increased levels of triglycerides are found in _______, _________, _______, _________

A

hyperlipidemias
diabetes
nephrotic syndrome
hypothyroidism

HDNH

38
Q

Increased levels are risk factor for ___________, ________, ________, ____________

A

arteriosclerotic coronary disease
peripheral vascular disease
acute pancreatitis
hyperlipoproteinaemia

APAH

39
Q

decreased levels are found in _________ and ___________

A

malnutrition and hyperthyroidism

40
Q

GPO
LPL
GK

A

glycerol 3 phosphate oxidase
lipoprotein lipase
glycerol kinase

41
Q

Triglycerides standard product code

A

1X 4 mL

42
Q

triglycerides standard description

A

triglycerides standard concentration
200 mg/dl

43
Q

give me the 9 under kit components

A

risk and safety
reagent preparation
reagent storage and stability
open vial stability
on board calibration stability
storage detoriation
precaution
waste management
sample

44
Q

Test parameters
Triglycerides

Mode of reaction
slope of reaction
wavelength 1
wavelength 2
temperature
Standard concentration
linearity
blank
incubation time
sample volume
reagent volume
cuvette

A

end point
increasing
546 - 540-560nm
630 nm
37 C
200 mg/dl
1000 mg/dl
reagent
5 minutes
10 ul
1000 ul
1 cm light path

45
Q

traditional unit
SI unit
Conversion from Traditional to Si

A

mg/dl
mmol/L
X0.0114

46
Q

Reference range in male and female triglycerides

A

Men 60-165 mg dl
Women 40 - 140 mg dl

47
Q

Sensitivity in triglycerides
Lower detection is ____

A

2 mg dl

48
Q

Calculation of triglycerides

A

Tri conce mg dl - absorbance of sample/ absorbance of standard X 200

49
Q

Linearity of triglycerides

A

1000 mg dl

50
Q

what is the methodology use by CHOLESTEROL

A

CHOD PAP - cholesterol oxidase phenol 4 aminoantipyrine peroxidase

51
Q

Linearity of cholesterol

A

600 mg dl

52
Q

Increased level of cholesterol are found in

A

Hypercholesterolaemia
Hypothyroidism
Hyperlipidaemia
Uncontrolled diabetes
Nephritic syndrome
Cirrhosis

53
Q

It is the main lipid found in the blood, bile, brain tissues

A

Cholesterol

54
Q

Decreased level of cholesterol

A

Malabsorption, malnutrition, anaemia, hyperthyroidism, liver disease

CLIN CHEM LAB (14 decks)

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