monoclonal antibody production Flashcards
1
Q
1st step
A
start with B cells from an immunized mouse
2
Q
2nd step
A
combine with myeloma cell
3
Q
3rd step
A
fuse B cell and myeloma cell
4
Q
4th step
A
select for cell that have fuse
5
Q
5th step
A
separate to single cell
6
Q
6th step
A
expand individual cell
7
Q
7th step
A
collect Ab from supernatant-> use for whatever purpose
8
Q
what cells proliferate forever
A
myeloma cells
9
Q
what type of cells survive drug treatment
A
only fused cells
