Molecular Targets Flashcards
1
Q
Target for HEV RNA
A
ORF2/3 overlap - detects all four genotypes
2
Q
What is the target for adenovirus PCR?
A
Hexon region
3
Q
What caused the diagnostic escape of clade I mpox in 2024?
A
Deletion in C3L gene of Clade Ib mpox which caused false negative results in commonly used typing assays
4
Q
Molecular target for enterovirus testing (and caveat!)
A
5’ non coding region (NCR)
Caveat - can cross react with rhinovirus
5
Q
What is the target for sequence typing enteroviruses?
A
VP1
6
Q
Target for influenza A PCR testing
A
Matrix gene - reasonably conserved across human and avian viruses
7
Q
Common real-time RT-PCR targets of SARS-CoV-2
A
Orf 1a/b
Envelope
Nucleocapsid
RdRp
