Lab stuff Flashcards
What are Levey-Jennings or Shewhart control charts?
How many points are required before use?
What is calculated?
Charts used to monitor lab controls
20
Mean and SD
What are 5 main Westguard rules?
Which require the run to immediately fail?
A - 1x 2SD
B - 2x 2SD
C - 4x 1SD
D - 1x 3SD
E - 10x one side of the mean
D
What parameters can be used in validation/verification?
Accuracy, sensitivity, specificity, reliability, repeatability reproducibility, precision, linearity (quant) and uncertainty of measurement
What types of quality assurance programmes are there?
What are 3 types of audits?
Internal (IQA) - 0.5-1% of all samples should be reanalysed
External (EQA) - inter lab comparisons
Horizontal, examination and vertical
What sort of things are stored in the quality management system?
SOPS
Validations/verifications
Audits
Non conformance (including CA/PA corrective and preventative action)
Equipment monitoring
Training records
EQA results
What is the packaging UN # and instructions for:
Cat A infectious substances
Cat B infectious substances
UN2814 packaging instructions 620
UN3373 packaging instructions 650
What is the UN # for:
Cat A medical waste
Cat B biomedical waste
UN3549
UN3291
What are the requirements for Cat A and Cat B packaging?
Leakproof primary receptacle
Leakproof secondary packaging
Absorbent material
Cushioning
Rigid out packaging (UN approved for Cat A)
What is a lab SLA?
Service level agreement
Basics of Sanger sequencing
Make up of VTM
Hanks balanced salt solution
FBS
Amphotericin B
Gentamicin
Sucrose
Phenol red
What can you use for inactivation for f viruses? Ie lysis buffer
Guanidium based chaotropic salts
Heat
UV
Detergents (eg Tween)
Use two eg guanadinium buffers and tween. Or heat for 1 h at 60C
