Molecular genetic part 2 Flashcards
WHAT IS ISOLATING GENES ON THE BASIS OF THEWIR POSITION ON A GENETIC MAP
Positional cloning
In _________, neighboring genes are used to locate a gene of iinterest
chromosome walking
what genetic disease is isolated by positional cloning
cystic fibrosis (AUtosomal Recessive disorder)
mRNA is strongly expressed in what part of the body affected by CF
Pancreas
what does the CF gene encode?
a protein called Cystic Fibrosis Transmembrane Regulator (CFTR)
How does CFTR function
as a chloride channel
who invented the dideoxy sequencing method
Frederick Sanger
dideoxy sequencing is based on
chain terminating substrates
what IS THE difference between dideoxyribonucleoside triphosphate (ddNTPs) and Deoxyribonucleoside triphosphate (dNTP)
ddNTP lacks a 3’OH group which causes chain termination and dNTP has a 3’OH group
Each of the four reaction contains
- single-stranded target DNA
- all four dNTP
- DNA polymerase
- primer
- one type of ddNTP
Examples of next-generation sequencing technologies
simultaneous determination
illumina sequencing
pyrosequencing
Third genertaion sequencing-Nanopore sequemcing
which DNA sequence uses DNA to identify individual people with some parts highly variable among individuals
DNA fingerprinting
this exhibits a variable number of copies of repeat sequences in different indivuduals
Microsatellites also called Short Tandem Repeats (STRs)
STRs are analyzed by
PCR and gel electrophoresis/ capillary electrophoresis
hwo can we identify people based on differences in their DNA?
the patterns of DNA fragements produced by individuals differ