Module 6 - 5 Flashcards
What are the two ways to regulate enzyme activity?
Controlling enzyme amount or adjusting activity of constant quantity
What are the ways to regulate enzyme availability?
Location, rates of synthesis and degradation
What is covalent modification?
Phosphorylation, methylation, glycosylation, etc.
What is non-covalent modification?
Allosteric regulation
What is negative feedback inhibition?
Final product inhibits enzyme catalyzing first unique and committed step
What is the logical point to regulate a reaction pathway?
At the first unique and committed step
What is the purpose of negative feedback inhibition?
Conserves material and energy, prevents accumulation of intermediates
What is the role of F in a branched pathway?
End product that inhibits enzyme catalyzing first unique and committed step
What is the role of A in a branched pathway?
Valuable and should be conserved unless F is needed
What is the role of B, C, D, and E in a branched pathway?
Intermediates in the production of F
What is the regulation when two pathways cooperate to form a single product?
Final product can inhibit the first unique step of each branch
What are the general properties of allosteric enzymes?
Information sensors, regulated by metabolic intermediates and modulators
What is the role of allosteric activators and inhibitors in PFK1 activity?
Modulate enzyme activity
What is covalent modification?
Regulation through modifying group
What is the most common post-translation covalent modification?
Phosphorylation
