MODULE 2 UNIT 4 Flashcards
group of protozoa that live in the blood and tissues of their human host and other vertebrates
hemoflagellates
move by means of flagella
hemoflagellates
hemoflagellates is from the family of
Trypanosomatidae
Hemoflagellates infecting man belong to two genera
Leishmania and Trypanosoma
heteroxenous
hemoflagellates
transmission of hemoflagellates
bite of an arthropod vector
four morphologic forms of clinical significance associated with hemoflagellates
amastigote, promastigote, epimastigote, and trypomastigote
amastigote was formerly called
leishmania
promastigote was formerly called
leptomonas
epimastigote was formerly called
crithidia
trypomastigote was formerly called
trypanosoma
It is also known as the L-D (Leishman-Donovan) body.
Amastigote
This stage is adapted to intracellular existence, being found inside macrophages, monocytes, neutrophils, or endothelial cells.
Amastigote
The body is slightly wider than that of the promastigote.
Epimastigote
Volutin granules are found in the cytoplasm.
Trypomastigote
shape and size: ovoidal in shape and, on an average, measures 5 µm long by 3 µm wide
Amastigote
All Leishmania species pass their life cycle in two hosts:
(1) man and other mammalian hosts; (2) insect vector
insect vector host of Leishmania
female sandlfy of the genus Phlebotomus in the Old World, and the genus Lutzomyia in the New World
Leishmaniasis is transmitted by the bite of infected female phlebotomine sandflies. 1) Identify the species; 2) Order of cycle
1) Leishmania; 1
The sandflies inject the infective stage (i.e., promastigotes) from their proboscis during blood meals 1) Identify the species; 2) Order of cycle
1) Leishmania; 2
Promastigotes that reach the puncture wound are phagocytized by macrophages and other types of mononuclear phagocytic cells. 1) Identify the species; 2) Order of cycle
1) Leishmania; 3
Promastigotes transform in these cells into the tissue stage of the parasite (i.e., amastigotes), which multiply by simple division and proceed to infect other mononuclear phagocytic cells 1) Identify the species; 2) Order of cycle
1) Leishmania; 4
Parasite, host, and other factors affect whether the infection becomes symptomatic and whether cutaneous or visceral leishmaniasis results 1) Identify the species; 2) Order of cycle
1) Leishmania; 5
Sandflies become infected by ingesting infected cells during blood meals 1) Identify the species; 2) Order of cycle
1) Leishmania; 6
In sandflies, amastigotes transform into promastigotes, develop in the gut (in the hindgut for leishmanial organisms in the Viannia subgenus; in the midgut for organisms in the Leishmania subgenus), and migrate to the proboscis 1) Identify the species; 2) Order of cycle
1) Leishmania; 7
The Trypanosoma brucei complexes pass their life cycle in two hosts:
(1) man and other mammalian hosts; (2) insect vector
trypanosomiasis vector
tsetse fly of the genus Glossina
During a blood meal on the mammalian host, an infected tsetse fly (genus Glossina) injects metacyclic trypomastigotes into skin tissue. The parasites enter the lymphatic system and pass into the bloodstream 1) Identify the species; 2) Order of cycle
1) Trypanosoma brucei complexes; 1
Inside the host, they transform into bloodstream trypomastigotes , are carried to other sites throughout the body, reach other body fluids (e.g., lymph, spinal fluid), and continue the replication by binary fission 1) Identify the species; 2) Order of cycle
1) Trypanosoma brucei complexes; 2
The entire life cycle of African trypanosomes is represented by extracellular stages. The tsetse fly becomes infected with bloodstream trypomastigotes when taking a blood meal on an infected mammalian host 1) Identify the species; 2) Order of cycle
1) Trypanosoma brucei complexes; 3
In the fly’s midgut, the parasites transform into procyclic trypomastigotes, multiply by binary fission , leave the midgut, and transform into epimastigotes 1) Identify the species; 2) Order of cycle
1) Trypanosoma brucei complexes; 4
The epimastigotes reach the fly’s salivary glands and continue multiplication by binary fission 1) Identify the species; 2) Order of cycle
1) Trypanosoma brucei complexes; 5
The cycle in the fly takes approximately 3 weeks.
African trypanosomiasis
An infected triatomine bug takes a blood meal and releases trypomastigotes in its feces near the site of the bite wound. Trypomastigotes enter the host through the bite wound or intact mucosal membranes, such as the conjunctiva 1) Identify the species; 2) Order of cycle
1) T. cruzi; 1
Inside the host, the trypomastigotes invade cells near the site of inoculation, where they differentiate into intracellular amastigotes
1) T. cruzi; 2
The amastigotes multiply by binary fission and parasitized cells rupture. 1) Identify the species; 2) Order of cycle
1) T. cruzi; 3
Some amastigotes enter further cells and others differentiate through transitional promastigotes and epimastigotes into trypomastigotes, and then are released into the circulation as bloodstream trypomastigotes 1) Identify the species; 2) Order of cycle
1) T. cruzi; 4
Trypomastigotes infect cells from a variety of tissues and transform into intracellular amastigotes in new infection sites where they again multiply by binary fission. The triatomine bug becomes infected by feeding on human or animal blood that contains circulating parasites
1) T. cruzi; 5
The ingested trypomastigotes transform into epimastigotes in the vector’s midgut 1) Identify the species; 2) Order of cycle
1) T. cruzi; 6
. The parasites multiply and differentiate in the midgut and differentiate into infective metacyclic trypomastigotes in the hindgut 1) Identify the species; 2) Order of cycle
1) T. cruzi; 7
Inhabit the macrophages of the skin and nearby lymph nodes.
Leishmania tropica complex
Leishmania tropica complex other names
L. tropica, L. aethiopica, and L. major
Leishmania braziliensis complex other names
L. braziliensis, L. panamensis, L, peruviana, and L. guyanensis
L. donovani complex other names
L. donovani, L. infantum, and L. chagasi
Leishmania mexicana complex
L. mexicana, L. amazonensis, and L. venezuelensis
Inhabit the macrophages of the skin, and mucous membranes of the nose and buccal cavity.
Leishmania braziliensis complex; Leishmania mexicana complex
Inhabit the reticuloendothelial cells of the viscera (liver, spleen, bone marrow, intestinal mucosa, and mesenteric venules)
L. donovani complex
Inhabit the endothelial cells of the kidneys, lungs, meninges and CSF
L. donovani complex
The term Old World is used to refer to
Asia, Africa, and Europe
refers to a part of the world that was known to its citizens before it came into contact with the Americans
Old World
New World refers to the Americas and it is inclusive of
North America, South America, and Central America
a general term used to describe diseases caused by genus Leishmania
Leishmaniasis
Old World cutaneous leishmaniasis (CL) agent
Leishmania tropica complex
Leishmania tropica complex other condition names:
- oriental sores - tropical sore - Aleppo button - Jericho boils - Delhi boils - Baghdad boils
causes cellular infiltration, necrosis and ulceration, and granuloma formation
Sandfly bite on the skin
characterized by one or more ulcers containing pus that generally self-heal
Cutaneous leishmaniasis
small red papule; 2 cm or larger in diameter; intense itching
Cutaneous leishmaniasis
crater-like with elevated and indurated margins
Skin ulcer
heals in 2-10 months even without treatment but leaves an ugly, dysfiguring scar
Skin ulcer
cause new world leishmaniasis in South and Central America
Leishmania braziliensis complex and Leishmania mexicana complex
Mucocutaneous leishmaniasis (ML) agent
Leishmania braziliensis complex and Leishmania mexicana complex
Leishmania braziliensis complex and Leishmania mexicana complex other condition names:
- espundia - forest yaws (AKA pian bois (L. guyanensis) - uta
Infection starts off as a reaction at the bite.
Mucocutaneous leishmaniasis (ML)
The skin lesion is similar to cutaneous leishmaniasis but often weeping ulcers.
Mucocutaneous leishmaniasis (ML)
It can go by metastasis into the mucous membrane of the nose, ears, pharynx and/ or larynx causing extensive dysfiguring lesion and can become fatal.
Mucocutaneous leishmaniasis (ML)
New World cutaneous leishmaniasis
Leishmania mexicana complex
New World cutaneous leishmaniasis other condition names:
- chiclero ulcer - bay sore
Infection leads to CL that resembles the Old World CL.
New World cutaneous leishmaniasis
chiclero ulcer characterized by ulcerations in pinna is caused by
L. mexicana
Visceral leishmaniasis (VL) agent
Leishmania donovani complex
Old World VL agent
L. donovani and L. infantum