Module 2 chapter 2 Flashcards
Basic components of living systems
What did schleiden discover
All plants were made of cells
What did Schwann discover
All animals were made of cells
What does the cell theory state
- Both plant and animal tissue is composed of cells
- cells are the basic unity of all life
- cells only develop from existing cells
What are the four types of sample preparation
- Dry mount
- Wet mount
- squash slides
- smear slides
Summarise Dry mount
-Solid specimens are viewed when been sectioned
-placed on slide with cover slip placed over it
-
Summarise Wet mount
- Specimens are suspended in a liquid such as water/immersion oil
- Cover slip is placed at an angle
Summarise Squash slides
- Wet mount is first prepared
- lens tissue gently presses down the cover slip
- Careful not to break the slip when pressing down
Summarise Slides
- The edge of the slide is used to smear the sample creating a thin even coating on another slide
- cover slip is placed over the sample
What sample preparation technique would you use to study: Hair pollen, dust, insect parts, muscle tissue, or plants
Dry mount
What sample preparation technique would you use to study: aquatic samples
Wet mount
What sample preparation technique would you use to study: cell division
Squash slides
What sample preparation technique would you use to study: Cells in blood
Smear slides
Why would you use staining in light microscopy
- normally low contrast as most cells no not absorb a lot of light
- Stains increase contrast in cells as different components of cell take up different stain degrees
- Resolution is limited by wavelength of light
How would you prepare a sample for staining
- Placed on slide and allowed to air dry
- Heat fixed by passing through flame
- specimen will adhere to the slide and take up the stain
Equation for magnification
Size of image divided by actual size of object
What is resolution
How clear the image is
What is contrast
The shading difference
What are the 2 types of electron mircoscopes
- Scanning electron microscope
- Transmission electron microscope
What magnification can electron microscopes go up to
over x500,000
How does a Transmission microscope work
A beam of electrons is transmitted through a specimen and focused to produce an image
How does a Scanning microscope work
A beam of electrons is sent across the surface of a specimen and the reflected electrons are collected
What is the transmissions resolving power
Best resolving power with 0.5nm
What is the scanning resolving power
resolving power is 3-10nm which isn’t as good but it produces 3D images`
Why would you see in more detail with an electron microscope than a light microscope
The electron wave length is smaller than a light wavelength therefore giving you a better image
What is an artefact
A visible structural detail that isn’t supposed to be there
What does differential staining do
Helps distinguish between two types of organisms that would otherwise be hard to identify.
What is the Gram stain technique used for
To separate bacteria into two groups, Gram positive and Gram negative
What will happen to Gram positive bacteria
retain the crystal violet stain and will appear blue or purple under the microscope
What will happen to Gram negative bacteria
thinner cell walls therefore lose the stain.
stained counterstain making them appear red
What are Gram positive bacteria susceptible to
Penicillin
Why are the microscopy slides brought into school ready-prepared
- Can be harmful
- long complex process needed to produce high quality sections
What is acid fast techniques used for
Differentiate species of Mycobacterium from other bacteria
What is a lipid solvent used for in the acid fast technique
Carries a carbolfuchsin dye into the cells being studied
What do you do for fixing in microscopy
chemicals like formaldehyde are used to perverse specimens in as near-natural state as possible
What do you do for sectioning in microscopy
specimens are hydrated with alcohols and then placed in a mould with wax or resin to form a hard block, then sliced thinly with a micotome
What do you do for staining in microscopy
specimens are often treated with multiple stains to show different structures
What do you do for mounting in microscopy
The specimens are then secured to a microscope slide and a cover slip placed on top