module 2 - cell structure and varieties Flashcards
what is the range of measurements in diameter of a cell, prokaryotes and eukaryotes?
cell: 1-100 um
pro: 0.2-2 um
euk: 10-100 um
what does the cell surface area determine?
amount of substances entering and exiting the cell
to function, cells must maintain what ratio?
large surface area to volume
who came up with the microscope?
Robert hooke
Mathias shleiden and Theodor Schwann worked on?
animal and plant tissues
what are light microscopes?
allows to see cell sizes and shapes and some internal structures
different types of light microscopes?
compound, stereo, digital, fluoroscent, inverted, automated
what are the 7 different types of microscopy?
- bright-field
- phase-contrast microscopy
- differential interference-contrast microscopy
- stained bright-field microscopy
- fluorescence microscopy
- confocal microscopy
- electron microscopy
what is bright-field microscopy?
a light passes through cells
what is phase-contrast microscopy?
contrast is increased and enhances light and dark regions in one cell
what is DIC microscopy?
cell is casting a shadow by 2 beams of light
what is stained BF microscopy?
stain enhances contrast and reveals details
what is fluorescense microscopy
fluorescent dye binds to specific cell material and is stimulated by beam of light
what is confocal microscopy?
focuses stimulated and emitted light so single plane through cell is seen
what is electron microscopy
directs electrons through a vacuum at fluorescent screen or digital camera to create a visible image (dead cells only)
what are the types of electron microscopy?
transmission electron microscope (TEM) and scanning electron microscopy (SEM)