Model Answers P2 Flashcards

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1
Q

Succession

A

• Pioneer species colonises an area with hostile conditions
• This leads to changes in the abiotic factors
• The conditions become less hostile
• Other species are able to colonise the area
• Conditions continue to change and become less hostile and new organisms outcompete the pioneer species increasing biodiversity
• Eventually conditions become favourable to a climax community
• The climax community has stable abiotic factors, stable populations, stable communities

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2
Q

Energy losses between trophic levels in Consumers

A

• Energy is not transferred between consumers because:
1. Some parts aren’t eaten (bones)
2. Some parts are eaten and are not absorbed (faeces)
3. Some parts are eaten, absorbed but excreted (urine)
4. Some biomass us broken down in respiration (temp regulation/movement)

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3
Q

Energy losses in plant GPP

A

• Sunlight is not converted to biomass because:
1. Some light is the wrong wavelength (e.g. green)
2. Some doesn’t hit a chlorophyll molecule/transmitted
3. Most light is reflected by other molecules in the atmosphere
4. Other limiting factors may be involved

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4
Q

Biomass

A

• Dry mass of carbon in a organisms in a particular area
• A sample of organism is dried.
• The sample is then weighed at regular intervals (e.g. every day) • Until the mass remains constant

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5
Q

Increasing Productivity on Farms

A

• Food chains/webs are simplified (pests are removed)
• Respiration of livestock is reduced (movement limited, temperature regulated)

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6
Q

Nitrogen cycle

A

• Nitrogen gas in the air is converted into ammonia in the soil by nitrogen fixing bacteria
• Some nitrogen fixing bacteria in leguminous plant root nodules have a mutualistic relationship with
plants and convert nitrogen gas to ammonia then nitrates directly
• Nitrates in the soil are absorbed by plant roots and converted to nitrogen containing compounds e.g. amino acids and DNA
• Nitrogen containing compounds in plants may be absorbed when eaten by consumers
• Proteins from waste and dead material are broken down/hydrolysed to ammonia in soil by enzymes
released by saprobionts during ammonification
• Ammonia in the soil is oxidised to nitrites, then nitrates, by nitrifying bacteria in the soil in aerobic conditions. These nitrates can be absorbed by the plants
• If the soil is waterlogged, the lack of oxygen leads to denitrification where nitrates are converted back to gaseous nitrogen by denitrifying bacteria

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7
Q

Optimising nitrogen cycle

A

• Using crop rotation to plant leguminous plants – these will replenish nitrates in the soil
• Using crop rotation to replenish soil nutrients
• Ploughing aerates soil to ensure more oxidised ammonia→nitrates in
nitrification
• Preventing waterlogging reduces anaerobic conditions so less denitrification occurs
• Selective breeding can be used to optimise growing conditions
• Fertilisers can be added to increase concentration of minerals e.g. nitrates

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8
Q

Phosphorus cycle

A

• Plants absorb phosphorous form the soil
• Consumers eat the plants and absorb phosphorous
• Dead and waste (faeces etc.) material is decomposed releasing phosphorous into the soil
• Runoff from farm fertiliser means excess phosphorous enters bodies of water (lakes/rivers etc.)
• Phosphorous sediment in water is uplifted forming rocks on the surface
• Weathering releases phosphorous from the rock into the water and soil
• Leeching of phosphorous from soil/weathered rock causes phosphorous to enter the water

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9
Q

Eutrophication

A

• Excess nitrates runoff into bodies of water
• Excess growth of algae/Algal bloom forms on the surface of water • Reduced light so aquatic plants die
• Saprobionts respire aerobically while decomposing dead matter
• Less oxygen for fish and other organisms so they die

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10
Q

LDR

A

• In photoionisation, light excites the electrons in chlorophyll II and they move to carrier proteins in the thylakoid membrane.
• The electrons are replaced by the e- produced by splitting water (photolysis), which also produces oxygen and H+.
• Electrons move along carrier proteins in a series of redox reactions losing energy as they go. This is used to pump H+ into the thylakoid space creating a chemiosmotic gradient.
• H+ move down the gradient through ATP synthase during photophosphorylation producing ATP from ADP+Pi
• The electrons are donated to chlorophyll I and more are excited by light, travelling along another ETC until they reduce NADP to NADPH with H+ from photolysis.

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11
Q

LIR

A

• CO2 is fixed, combining with RuBP using the enzyme Rubisco
• This produces two glycerate-3-phosphate (GP)
• GP is reduced to Triose phosphate (TP)
• Using energy from ATP and reduced NADP
• TP can be regenerated to RuBP using energy from ATP,
• 1C from TP is converted into organic molecules e.g. glucose, amino acids, glycerol

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12
Q

Glycolysis

A

• In the cytoplasm
• Phosphorylation of glucose using ATP to make it more reactive;
• Lysis of the phosphorylated glucose intermediate to form Triose Phosphate
• Oxidation from TP to pyruvate by losing H+ and e-
• Net gain of 2 ATP;
• NAD reduced/NADH formed

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13
Q

Link reaction

A

• In the mitochondrial matrix
• Pyruvate is oxidised using coenzyme A • CO2 released
• NAD is reduced
• Acetyl CoA is formed

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14
Q

Krebs cycle

A

• Acetyl CoA reacts with a 4C acceptor molecule
• The 6C intermediate is decarboxylated and oxidised,
removing CO2 and reducing NAD
• The resulting 5C intermediate is also decarboxylated and oxidised removing CO2 and reducing 2xNAD, reducing FAD and generating 1x ATP in a series of REDOX reactions.
• Until the original 4C acceptor is formed again.

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15
Q

Oxidative phosphorylation

A

• FADH and NADH are oxidised and lose e- and H+
• The e- are passed from carrier protein to carrier protein in the mitochondria inner
membrane in a series of redox reactions
• This releases energy
• The energy is used to pump H+ through the membrane into the inner membrane space building a chemiosmotic gradient
• H+ moves back through the membrane through ATP synthase
• ADP+Pi→ATP
• Oxygen is the terminal electron acceptor forming water

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16
Q

Anaerobic respiration in mammals

A

In Mammals
• Pyruvate is reduced to lactate • NADH is oxidised during this
process
• This prevents NAD running out and allows ATP to continue being made in glycolysis

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17
Q

Anaerobic respiration in yeast

A

In yeast
• Pyruvate is reduced to ethanal then ethanol
• NADH is oxidised during this process
• CO2 is produced
• This prevents NAD running out and allows ATP to continue being made in glycolysis

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18
Q

Taxis

A

• In invertebrates
• Movement in a direction
• Movement toward (positive) or away (negative) from stimulus
• So organisms can survive and reproduce
• E.g. chemotaxis, phototaxis,

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19
Q

Kinesis

A

• In invertebrates
• Directionless movement
• Movement isn’t in a direction
• Usually, to do with rate of turning
• Increased rate of turning leads to an organism remaining in favourable conditions
• So organisms can survive and reproduce

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20
Q

How does IAA impact cell elongation in roots

A
  1. IAA produced in the tip
  2. IAA diffuses down the root
  3. IAA accumulates/moves to the base of the root (due to gravity)
  4. IAA inhibits elongation in root
  5. Root elongates downwards
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21
Q

How does IAA impact cell elongation in the shoots

A

Shoots (when sunlight from one direction)
1. IAA produced in tip
2. IAA diffuses down the shoot
3. IAA accumulates/moves to the shaded side
4. Leading to cell elongation
5. Shoot elongates toward the light

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22
Q

Pacinian corpuscle

A

• Pressure is applied and the lamella is deformed
• Stretch mediated sodium ion channels open
• Na+ diffuse into axon
• Leading to depolarisation and action potential if threshold is exceeded

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23
Q

Convergence in rod cells

A

• Rods are found around the outside of the retina, away from the fovea
• Light stimulus triggers depolarisation in the rod cells
• Many rods converge and are connected to a single sensory neurone
• Depolarisation undergoes (spatial) summation to trigger an action potential
• In low light there is enough light to lead to action potential
• However, this reduces visual acuity

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24
Q

Acuity in Cone Cells

A

• Cone cells are located in the fovea (behind the retina to maximise light stimulus)
• Every cone cell is connected to a single sensory neurone (no convergence)
• Each cone sends a single impulse to the brain
• With high visual acuity
• Three types of cones detect three different wavelengths of light

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25
Q

Control of heart rate/initiating a heart beat

A

• Sino Atrial Node initiates a wave of electrical impulses across both atria causing them to contract
• Non-conductive tissue prevents impulse going straight to ventricle
• Atrio Ventricular Node delay impulse so ventricles can fill
• AV Node sends wave of electrical impulses down Bundle of His
• Ventricles contract from bottom up.

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26
Q

What happens if CO2 (pH)increased

A

• pH in blood lowers
• Chemoreceptors detect it
• CO2 needs to be removed
• Sensory neurone takes more impulses to medulla
• Medulla sends more impulses along the sympathetic
nerve
• Sympathetic nerve causes SAN to increase
• Heart rate increases

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27
Q

What happens if the CO2 (pH) decreases?

A

• pH in blood increases
• Chemoreceptors detect it
• Sensory neurone takes more impulses to medulla
• Medulla sends more impulses along the parasympathetic
nerve
• Parasympathetic nerve causes SAN to decrease
• Heart rate decreases

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28
Q

What happens if the blood pressure increases?

A

• Baroreceptors detect pressure increase
• Too high pressure can damage artery walls, so needs to be
restored
• Sensory neurone takes more impulses to medulla
• Medulla sends more impulses along the parasympathetic
nerve
• parasympathetic nerve causes SAN to decrease
• Heart rate decreases

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29
Q

What happens if the blood pressure decreases?

A

• Baroreceptors detect pressure increase
• Too low pressure means not enough oxygen etc. will get
to cells
• Sensory neurone takes more impulses to medulla
• Medulla sends more impulses along the sympathetic
nerve
• sympathetic nerve causes SAN to increase
• Heart rate increases

30
Q

Differences between nervous and hormonal communication

A

• Nervous is fast
• Response is short lived
• Requires neurone, neurotransmitters and nervous impulses
• Hormonal is slow
• Response is long lasting
• Hormones are produced in glands, travel in the blood to an organ/tissue with a target receptor

31
Q

Maintenance/Restoration of resting potential

A

• Na+/K+ pumps 3 Na+ out of the axon and 2 K+ into the axon using ATP
• Membrane is more permeable to K+ so K+ moves out through channels and less permeable to Na+
• Axon has a more negative potential difference inside

32
Q

Refractory period

A

• The period between firing of one action potential and the next
• The axon is hyperpolarised (very negative) on the inside
• Refractory period ensures:
• Action potentials occur in one direction • Limits number of action potentials
• Keeps action potentials separated

33
Q

Action potential

A

• Na+ ions enter the axon (e.g. Na+ receptors at synapses, stetch mediated-sodium chnnels)
• The axon membrane is depolarised
• Potential difference across axon becomes more
positive
• Potential difference will reach threshold
triggering action potential
• Voltage gated Na+ channels will open
• Na+ moves into the axon by facilitated diffusion
• Na+ channels close, K+ channels open
• K+ leaves the axon by facilitated diffusion – axon is repolarised
• Axon membrane becomes hyperpolarised in the refractory period
• Na+/K+ pump restores resting potential

34
Q

Effect of drugs or inhibitory NT: More chloride entering post synaptic neuron

A

More chloride entering post synaptic neuron
• Cl-are negatively charged
• They make the post synaptic
neurone hyperpolarised
• More Na+ needs to enter the post synaptic neuron for depolarisation of the neurone to pass threshold
• Action potential is inhibited

35
Q

Effect of drugs or inhibitory NT: More potassium ions leaving the post synaptic neuron

A

• K+ are positively charged
• When they move out they make the post synaptic neurone hyperpolarised
• More Na+ needs to enter the post synaptic neuron for depolarisation of the neurone to pass threshold
• Action potential is inhibited

36
Q

Impact of drugs that bind receptors/enzymes

A

• Drugs bind receptors instead of Ach because they are complementary – they prevent Na+ from entering, threshold isn’t reached reducing action potentials.
• Drugs prevent acetylcholinesterase from breaking down Ach by aching as an inhibitor (inhibitor model answer applies here e.g. blocks active site or changes shape so the active site is no longer complementary) this prevents Ach from being removed from receptors, more Na+ diffuses in increases action potentials.
• Drugs may be complementary to the NT (substrate) and bind it, this changes shape of the substrate and means it can’t bind the receptor. Less Na+ moves in, doesn’t pass threshold reducing action potentials.

37
Q

Synapses

A

• Action potential reaches pre-synaptic membrane allowing Ca2+ channels to open and Ca2+ enters by facilitated diffusion.
• Vesicles containing Acetylcholine (Ach) fuse with the pre-synaptic membrane.
• Ach diffuses across the synaptic cleft
• Ach binds to the receptors on the post-synaptic membrane.
• Na+ channels open and Na+ moves in by facilitated diffusion
• Leading to depolarisation
• Acetylcholine esterase hydrolyses Ach to acetic acid and choline to be returned to the presynaptic neurone

38
Q

Neuromuscular junction

A

(events before this are the same as a synapse)
• Vesicles of neurotransmitter fuse with the pre-synaptic membrane
• Neurotransmitter e.g. Acetylcholine diffuse across the neuromuscular junction
• Neurotransmitters bind to the Na+ receptors causing Na+ to enter the sarcolemma (muscle tissue) depolarising it
• Depolarisation is transmitted through the T-tubules causing Ca2+ ions to be released from the sarcoplasmic reticulum
• Ca2+ binds to tropomyosin in the leading to muscle contraction

39
Q

Muscle Contraction

A

• Calciumionsdiffuseintomyofibrilsfromthesarcoplasmicreticulum
• Ca2+bindstotropomyosinchangingitsshapeandmove
• Thischangeexposesthemyosinbindingsitesontheactin
• Myosin‘heads’bindtotheActinformingacrossbridge.
• Ca2+activatesATPasehydrolysingATP.Myosin‘heads’tiltinarowingmotion pulling actin molecules.
• ATPcannowbindtotheMyosinhead,changing theshapeandbreakingthe cross bridge
• ATPishydrolysedbytheheadprovidingenergyforthe headtoflipback/re- cock
• ADPandPiremainsonthehead

40
Q

Hormones

A

Hormones are released by glands
They travel in the blood
They bind to complementary receptors on specific target cells

41
Q

Hormonal control of glucose:insulin

A

• Binds to specific receptors in the liver and muscles
• Increases permeability of membranes to glucose – carrier proteins are activated
• Activates enzymes to turn glucose into glycogen in glycogenesis (in liver for storage)
• Increases the rate of respiration in muscles
• Increases the conversion of glucose into fat

42
Q

Hormonal control of glucose:glucagon

A

• Binds to specific receptors in the liver and muscles
• Activates enzymes to turn glycogen into glucose (especially in the liver) in glycogenolysis
• Promotes making glucose form fatty acids and amino acids in gluconeogenesis
• Decreases the rate of respiration in muscles

43
Q

Second messenger model

A

• Adrenaline binds to the specific receptors on target cells (the liver)
• The enzyme adenyl cyclase changes shape and is activated
• Adenyl cyclase catalyses conversion of ATP to cyclic AMP
• Cyclic AMP activates a protein kinase
• Cyclic AMP and protein kinase stimulates glycogenolysis (conversion of glycogen to glucose)
This provides more glucose for respiration

44
Q

Ultrafiltration

A

• Blood passes through the afferent arteriole to the glomerulus
• The efferent arteriole has a narrower lumen so this generates a high
hydrostatic pressure
• Small molecules (ions/water/glucose) are forceds through gaps in the capillary endothelium
• They pass through the basement membrane into the renal capsule
• Large proteins and cells remain in the blood

45
Q

Selective réabsorption

A

• Na+ is actively pumped out of the cell into the blood by the sodium potassium pump
• This lowers the concentration of sodium in the Proximal convoluted tubule
• Na+ moves into the cell from the lumen by facilitated diffusion and glucose/amino acids/salts are co-transported with it
• Glucose/amino acids/salts are then transported into the blood by facilitated diffusion
• Absorption of the solutes increased the water potential in the lumen
• Water is absorbed from the proximal convoluted tubule to the blood by osmosis

46
Q

Co transport

A

Co-Transport
• Na+ is actively pumped out of the cell into
the blood by the sodium potassium pump
• This lowers the concentration of sodium in
the epithelium cell
• Na+ moves into the cell from the lumen by facilitated diffusion and glucose/amino acids are co-transported with it
• Glucose/aminoacidsarethentransported into the blood by facilitated diffusion

47
Q

Loop of henle

A

▪ Water cannot move out of the ascending limb. Na+ and Cl- are ACTIVELY TRANSPORTED out of the ascending limb of the loop of Henle.
▪ Water moves out of the descending limb via Osmosis due to the high conc of Na+ / Cl- (lowering the water potential) in the tissue fluid. Na+ moves into the descending limb.
▪ This loss of water means that the conc of Na+ / Cl- is increased (less dilute solution).
▪ As the fluid moves down the descending limb and start to ascend, Na+ / Cl- diffuse out of the loop.
▪ This causes more water to be lost from the lowest descending part of the loop via osmosis.
▪ The longer the loop, the greater the water potential gradient so more can be absorbed.

48
Q

ADH: dehydrated

A

If someone is Dehydrated
• Water potential in the blood decreases
• Osmoreceptors in the hypothalamus shrink
• Stimulates the hypothalamus
• Increases ADH release by posterior pituitary gland
• ADH carried in the blood to the distal convoluted tubule and the collecting duct

49
Q

Role of ADH in the collecting duct and distalCT

A

• ADH binds to complementary receptors on specific target cells in the collecting duct and distal convoluted tubule (DCT)
• The enzyme phosphorylase is activated by this binding
• Leading to aquaporins fusing with the membrane of the epithelial
cells in the collecting duct (DCT)
• Membrane permeability to water increases
• Water moves from a high water potential in the collecting duct to a low water potential in the blood via osmosis
• This leads to increased concentration of urine (so less urine is produced)

50
Q

Role of ADH in the collecting duct and distalCT model answer – in entirety!

A

• Water potential in the blood decreases
• Osmoreceptors in the hypothalamus shrink
• Stimulates the hypothalamus
• Increases ADH release by posterior pituitary gland
• ADH carried in the blood to the distal convoluted tubule and the collecting duct
• ADH binds to complementary receptors on specific target cells in the collecting duct and distal convoluted tubule (DCT)
• The enzyme phosphorylase is activated by this binding
• Leading to aquaporins fusing with the membrane of the epithelial cells in the collecting
duct (DCT)
• Membrane permeability to water increases
• Water moves from a high water potential in the collecting duct to a low water potential in the blood via osmosis

51
Q

Reverse transcriptase

A

• Mature mRNA is extracted and converted to cDNA by Reverse Transcriptase
• cDNA is converted to double stranded DNA by DNA polymerase
• mRNA is found in large amounts in cells and contains no introns, so the gene products are easily expressed even by bacteria

52
Q

Restriction endonuclease

A

• DNA is cut at specific recognition sites
• Cuts can form ‘sticky ends’ these are complementary and can be used for joining DNA together

53
Q

Gene machine

A

• Desired gene sequence is designed using a computer – it is checked for safety and standards.
• Small pieces of DNA (oligonucleotides) are synthesised and joined together to make a sequence of DNA
• Using sticky ends these pieces of DNA can be inserted into a vector that can be used in cloning.
• Gene machines are quicker as they don’t need to isolate DNA/mRNA first

54
Q

Genetic engineering

A

• Isolate DNA/mRNA from an organism using a restriction endonuclease or reverse transcriptase to get DNA
• Cut plasmid and isolated DNA with the same restriction endonuclease to get complementary sticky ends
• A promoter and terminator region may need to be added to the gene
• Use DNA ligase to join the desired gene to a plasmid – forming phosphodiester
bonds
• Include marker gene e.g. antibiotic resistance
• Transform host using Ca2+ ions and a heat shock so plasmid passes through the membrane
• Allow bacteria to grow (colonies) then (replica) plate onto medium where the marker gene is expressed
• Bacteria / colonies not killed have antibiotic resistance gene/don’t fluoresce/have enzyme action and (probably) the wanted gene;

55
Q

– Polymerase Chain Reaction

A

Method
•DNAheatedto90to95°Ctoseparatethestrands
•DNA is cooled to 55°C so that primers can bind
•Free nucleotides attach by complementary base pairing
•Temperature is increased to 72°C and DNA polymerase joins nucleotides together forming a phosphodiester bond
•Cycle is repeated and DNA is copied at an exponential rate

56
Q

Gel electrophoresis

A

• DNAiscutatareasoftandemrepeatsusingrestriction endonucleases
• DNAfragmentsareplacedinwellsatthetopofanagargel.
• An electric current is applied over it.
• DNA is negatively charged due to the phosphate group
• TheDNAmovestowardsthepositiveelectrode,butatdifferent rates.
• Smallfragmentsmovefurtherthroughthegel
• A ladder/marker can be used to determine the size of the DNA fragments

57
Q

DNA fingerprinting

A

• Extracted DNA is cut with a restriction endonuclease at sites of variable number tandem repeats/minisatellites
• DNA is separated by gel electrophoresis, shorter fragments run further on the gel
• Use Southern Blotting to transfer DNA to a nylon membrane
• Use an alkaline solution to make DNA single stranded
• Add a single stranded probe tagged with radioactive/fluorescent molecule
• Visualise the DNA using and X-ray film or UV light

58
Q

Types of mammal stem cells

A

• Totipotency – can specialise into any type of cell including placenta, found in early stages of an embryo
• Pluripotency – can specialise into most types of cells, but not form placenta, found in early embryos but not the earliest
• Multipotency – can form some types of cells e.g. red and white blood cells, found in adult mammals
• Unipotency – can only form one type of cell, found in adults

59
Q

Transcription factor

A

• A transcription factor is a protein that binds the promoter region of DNA to turn on Gene Expression.
• They recruit RNA polymerase to bind the promoter.
• Transcription can be controlled by:
• Inhibitors binding the TFs changing their shape so they can’t bind to the promoter
• Sometimes other molecules (e.g. proteins/phosphates/steroid hormones) need to bind to change the shape of the TF to help it to bind

60
Q

Oestrogen as a Transcription Factor

A

• Oestrogen is a steroid hormone so is lipid soluble so diffuses across the phospholipid bilayer
• Oestrogen binds to the complementary receptor on the transcription factor
• Binding causes a change in the tertiary structure
• The transcription factor moves into the nucleus through the nuclear pore
• The transcription factor can now bind to a specific promoter base sequence of DNA
• Binding of the transcription factor switches the gene on by encouraging RNA polymerase to bind and starting transcription

61
Q

SiRNA

A
  1. dsRNA is cut by an enzyme into small dsRNA sections called siRNA (small interfering)
  2. siRNA becomes ssRNA and combines with an enzyme
  3. The complementary ssRNA binds to mRNA with the enzyme
  4. The mRNA is cut
  5. NO TRANSLATION
62
Q

Proto-oncogenes

A

Mutation changes the proto-oncogene into an oncogene:
• Receptor protein permanently activated
• Oncogene may code for a growth factor that is produced in large amounts
• Excessive uncontrolled cell division

63
Q

Tumour Suppressor Gene

A

• Mutation changes the sequence of the bases in the tumour suppressor gene.
• Tumour suppressor gene is no longer transcribed or tertiary structure of protein is altered
• Cell division is no longer controlled
• (This is often caused by increased methylation, but we’ll get to that when we do epigenetics)

64
Q

Epigenetics:decreased acetylation

A

Decreased Acetylation
• Histone Deacetylase removes acetyl groups from histones
• Increases the positive charges on histones
• Increases their attraction to the negative phosphate groups.
• Association between histone and DNA is stronger so Tighter coiling
• Transcription factors and RNA Polymerase can’t bind as easily
• Transcription of gene less likely and gene is switched off

65
Q

Epigenetics: increased methylation

A

Increased Methylation
• Methylation is addition of methyl group to cytosine bases of DNA
• DNA coils more tightly
• Transcription factors and RNA
Polymerase can’t bind as easily
• Transcription of gene less likely and gene is switched off
Epigenetics often impacts tumour suppressor genes by turning them off leading to uncontrolled cell division

66
Q

Chromatography

A

• Draw a line on chromatography paper using a pencil so it doesn’t dissolve
• Add the solution to the line in a small dot using suitable equipment
• Add the chromatography solvent to below the pencil line
• Remove from the chromatography paper from solvent before the solvent front reaches the end of the paper
• Mark where the solvent front reaches
• Calculate RF values
Rf = Distance travelled by solute (pigment) /Distance by solvent front

67
Q

– Chlorophyll Dehydrogenase (DCPIP)

A

• DCPIP is blue when oxidised and colourless when reduced.
• Becoming colourless quicker indicates photosynthesis is quicker
• Extract chloroplasts using cell fractionation method (cold isotonic buffered)
• Set up controls without light to check if it is photosynthesis, no chloroplasts and no DCPIP to use as a colour standard for the end point
• Time how long it takes for a colour change

Rate= 1/Time

68
Q

– Respiration (Respirometer)

A

• Use a respirometer (capillary tubing) to measure the volume of gas during respiration, this is based on how far the liquid moves
• Set up using a substance to absorb CO2 e.g. soda lime or KOH
• Equipment must be set up and left to equilibrate – enzymes adjust to temperature, changes in equipment settle
• Can be done with Methylene Blue – this is reduced by electrons and goes colourless (similar to DCPIP)

69
Q

Animal movement-maggots

A

• Set up your investigation, you need a large sample size to be representative.
• Ensure the placement of the organism is random (e.g. which wat the head is pointing)
• Collect data from which segment of the equipment the organism is in
• Conduct a statistical test e.g. chi2
• Repeat and calculate mean/SD

70
Q

Calibration curve

A

• Colour is subjective so use a colourimeter
• Set up a dilution series of known concentrations
• Complete the reaction (e.g. Benedicts test)
• Use a colorimeter to determine the absorbance/transmittance of the known concentration solutions to use as a colour standard/comparison
• If there is an end point of the reaction the absorbance will be a reference to the end point
• Plot the results on a graph and interpolate (read off the graph against transmission/absorbance) to determine the unknown

71
Q

Quadrat

A

• Use quadrats in either random sampling or a transect
• A transect is for changing conditions – you would place quadrats along at regular intervals
• Random sampling: Use a random number generator to generate cooordinates and place your quadrat in a grid
• Count the number of species in your quadrats and multiply by the total area