Cell Fractionation Flashcards
1
Q
What is a centrifuge
A
Devise that separates organelles by density by spinning
2
Q
What is a Homogeniser
A
A blender which breaks cell walls and membranes releasing organelle
3
Q
What 3 things must homogenate be
A
COLD reducing enzyme activity which breaks down organelle
ISOTONIC prevent organelles bursting or shrivelling due to osmosis
BUFFERED constant pH prevents proteins and enzymes denaturing
4
Q
Why is homogenate filtered
A
Remove large cellular debris
5
Q
Differential centrifugation produces
A
Pellet of densest organelle
Supernatant
6
Q
Most to least dense organelle
A
Nucleus
Mito and chloro
ER Golgi lysosomes
Ribosomes
