Microscopy Ch.3 Flashcards
10nm and 20 thousand times magnification
SEM
2nm resolution and 2 milllion times magnification
TEM
Alternating light and dark areas contrasting internal cell structures
TEM
Can observe arrangement, shape, and size of killed microorganism
Bright-Field Microscopy
Can observe internal structures of live, unstained eukaryotic microorganisms
Phase-Contrast Microscopy
Can observe live, unstained microorganisms; motility of live cells
Dark-field Microscopy
Can observe outline of microorganisms coated with fluorescent-tagged antibodies
Fluoresence Microscopy
Fluorescing microorganisms on dark background
Fluoresence Microscopy
Microbial surfaces
SEM
Stained microorganisms on clear background
Bright-Field Microscopy
Surfaces and textures of microorganisms and cell components
SEM
Ultrathin slices of microorganisms and internal components
TEM
Unstained microorganism on dark background
Dark-field Microscopy
Unstained microorganism with contrasted structures
Phase-Contrast Microscopy