Microscopy and Staining Techniques Flashcards
It is an optical microscope that uses light rays to produce a dark image against a bright background.
Brightfield Microscope
The condenser directs the light obliquely so that the light is deflected or scattered from the specimen, which then appears bright against a dark background.
Darkfield Microscope
When light passes through cells, small phase shifts occur, which are invisible to the human eye. In this microscope, these phase shifts are converted into changes in amplitude, which can be observed as differences in image contrast.
Phase-Contrast Microscope
It is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic substances.
Fluorescent Microscope
It is a microscope that uses a beam of accelerated electrons as a source of illumination.
Electron Microscope
If you want to know the shape or size of a microbe without targetting a specific structure, what time of staining can you use?
Simple staining
Malachite green is used in what staining technique?
Endospore Stain
In endospore staining, the vegetative cell will have red while the spore stain will have what color?
Green
Carbol fuschin is a stain specific for?
Mycobacterium
If gram negative bacteria stains red, what stain will gram positive bacteria have?
Purple
It is the part of a microscope that controls the amount and intensity of light
Diaphragm
Lactophenol blue can be used to stain what microbe?
Fungi
The secondary stain in gram stain is?
Saffranin
What is the purpose of heat fixing a bacterial smear before staining?
To kill and adhere the bacteria to the slide
What is used as a decolorizer in the Ziehl-Neelsen acid fast staining method?
Acid-alcohol
