Microscopy and Staining Techniques Flashcards
It is an optical microscope that uses light rays to produce a dark image against a bright background.
Brightfield Microscope
The condenser directs the light obliquely so that the light is deflected or scattered from the specimen, which then appears bright against a dark background.
Darkfield Microscope
When light passes through cells, small phase shifts occur, which are invisible to the human eye. In this microscope, these phase shifts are converted into changes in amplitude, which can be observed as differences in image contrast.
Phase-Contrast Microscope
It is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic substances.
Fluorescent Microscope
It is a microscope that uses a beam of accelerated electrons as a source of illumination.
Electron Microscope
If you want to know the shape or size of a microbe without targetting a specific structure, what time of staining can you use?
Simple staining
Malachite green is used in what staining technique?
Endospore Stain
In endospore staining, the vegetative cell will have red while the spore stain will have what color?
Green
Carbol fuschin is a stain specific for?
Mycobacterium
If gram negative bacteria stains red, what stain will gram positive bacteria have?
Purple
It is the part of a microscope that controls the amount and intensity of light
Diaphragm
Lactophenol blue can be used to stain what microbe?
Fungi
The secondary stain in gram stain is?
Saffranin
What is the purpose of heat fixing a bacterial smear before staining?
To kill and adhere the bacteria to the slide
What is used as a decolorizer in the Ziehl-Neelsen acid fast staining method?
Acid-alcohol
What reagent is used as a decolorizer in gram stain?
Alcohol or acetone
In gram stain, which reagent is used as primary stain?
Crystal Violet
What is the primary stain used in acid fast staining?
Carbol Fuschin
What is the function of the course focus knob?
Moving the stage
What staining procedure do you use in mycobacterium species?
Acid-fast stain
What color do acid-fast bacteria appear after acid staining?
Red
What is the role of saffranin in endospore staining?
To counterstain vegetative cells
Which stain is commonly used to visualize fungi in clinical specimens
Lactophenol cotton blue
Which objective lens provide the highest magnification on a compound microscope?
100x oil immersion
What is the purpose of iodine in gram staining?
To act as a mordant, forming a complex with crystal violet
What part of the microscope is used to focus the light to the specimen
Condenser
What stain is used to highlight capsules of certain bacterias?
Capsule stain
What is the purpose of counterstain in acid fast staining procedure?
To stain non acid fast bacteria
What staining method is used to detect fungal elements in tissues and clinical specimens?
Periodic acid schiff (PAS) stain
