LAB Quiz 3 - Media Flashcards
Vitamins, amino acids and nucleotides must be added to an enriched medium because these will act as ____ source.
growth factor
Staphylococcus aureus have yellow colonies in Mannitol Salts Agar while Staphylococcus epidermis have red colonies, this is an example of:
differential media
If you want to know the shape or the size of the microoganism without targeting its specific structure, you can use:
simple staining
A tryptic soy agar which is a general purpose media can be added with 5% sheep blood agar to make it _____ for cultivation of picky eaters of bacteria.
enriched media
Malachite green is used in what specific staining technique?
endospore stain
A microscope used in laboratory which uses dyes to target specific structure or organic substances in the sample.
fluorescent microscope
If you are asked to prepare a 500 ml Nutrient broth (40 grams in 1000ml), how many grams of Nutrient agar should you weigh?
20 grams
The main difference of a semi solid or solid media from liquid media is:
presence of agar
Gram positve bacteria such as Staphylococcus aureus can grow in Mannitol Salts Agar but gram negative bacteria cannot, this is an example of:
selective media
In endospore staining, the vegetataive cell will have a red color while the spore will stain:
green
Carbol fuschin is a stain specific for viewing what type of micoorganism?
Mycobacterium
It is an important material in microbiology which is used to cultivate, isolate and store microorganisms.
culture media
In yield constant, if you needed a total dry cell mass of 1 gram, you must add how many carbon/glucose in the media?
2 grams
The parameters in order to achieve sterility of glasswares or culture media using an autoclave is:
121 degrees celsius for 15 minutes at 15 psi
All gram-negative bacteria will stain red while all gram positive bacteria will stain purple. True or false?
True
