Microbial Cell Growth & Contributions Flashcards
T or F: Bacterial Media contains nutrients; glucose
True
T or F: Nutrient agar consists of essential nutrients that support the growth of bacteria
True
T or F: A single bacteria cell has 55% carbon, 17% oxygen, 13% nitrogen, 8.2% Hydrogen, 2.5 phosporus, 1.8% sulfur, <0.01 selenium
False, carbon is only 50%
T or F: When bacteria eats the nutrient in the agar, it multiples
True
T or F: Carbon has the highest contribution in bacteria cell
True
T or F: Protein is the secondary contributor of macromolecules in a composition of a cell
False, protein is the primary contributor
It breaks down glucose molecules into ATP to get energy to replicate and produces energy
Catabolism
It builds up molecules to have new set of cells but loses energy; uses energy
Anabolism
Cell’s chemical formula: ???
CH2 O0.5 N0.15
single cell splits into two equal cell
Binary fission
asexual reproduction where a part of the cell is pinched off where a daughter cell may arise
Budding
asexual reproduction, for dispersal and survival
Spores
Duplication of cell
Logarithmic phase
Rapid increase curing a constant time interval
Exponential growth
adaptative phase where synthesis of RNA, enzymes and essential metabolites not found in the environment are produced, cells are adjusting to the culture medium (pH, tempt., O2 conc.) and damaged cells are undertaking repair
Lag Phase
Also called log phase, cell undergo division (doubling of cells = rapid growth). It is the stage where cells are healthiest
Exponential Phase
No increase or decrease in cell number, cells try to adapt to starvation, plasma membrane becomes less fluid and permeable, nucleoid condenses, DNA with binding proteins for preservation. The stage where secondary metabolites are produced
Stationary Phase
Cell number decreases in a fast rate , lack of nutrients and accumulation of toxins or by products. Cells break open or lyse and spill their contents, some try to mutate or adapt for survival
Death Phase
Essential for growth and reproduction
Primary Metabolites
Used for protection
Secondary metabolites
T or F: All bacteria have the same death phase
False
It uses the petroff-housser counting chamber
Direct microscopic counting
Quantifies the growth of bacteria, plating counting number of bacterias. For ex., serial dilution, spread plate, pour plate
Viable count
Measurements are made in spectrophotometer. In this method it measures incident light unscattered by cells in suspension and gives readings in optical density units
Turbidimetric method
