Microscopy

Question 1

Magnification?

Answer 1

Number of times an image is bigger than the object from which it was derived

Question 2

Resolution?

Answer 2

The smallest distance that can be distinguished as two separate points in a microscope

Question 3

Why are electron microscopes higher resolution than light microscopes?

Answer 3

Electrons have shorter wavelengths than visible light so distinguish objects that are smaller and closer together

Question 4

What are the different planes of cutting a sample?

Answer 4

Transverse (vertical) and longitudinal (horizontal)

Question 5

Advantages of electron microscopes

Answer 5

Higher resolution
higher magnification

Question 6

disadvantages of electron microscopes

Answer 6

specimens must be dead
only back and white
expensive
required extensive training to use

Question 7

advantages of light microscopes

Answer 7

Can observe live specimens
Can see colour images
Doesnt require training
Cheaper

Question 8

disadvantages of light microscopes

Answer 8

lower resolution and magnification