Microscopy

Question 1

A differential staining technique based on bacterial cell wall structure

Answer 1

Gram stain

Question 2

A differential staining technique based on bacterial cell wall structure Classifies bacteria as:

Answer 2

GRAM-POSITIVE
GRAM-NEGATIVE

Question 3

Primary stain of Gram stain

Answer 3

Crystal violet

Question 4

Mordant of Gram stain

Answer 4

Grams Iodine

Question 5

Decolorizer of Gram stain

Answer 5

Acetone-alcohol

Question 6

Counter stain of gram stain

Answer 6

Safranin

Question 7

Fungal elements appear as

Answer 7

Gram-positive or Gram-variable

Question 8

Examine for —- to confirm if yeast cells and not bacteria or
artifact

Answer 8

budding cells

Question 9

India ink is used for

Answer 9

Cerebrospinal fluid

Question 10

stain is the background, yeast cells remain unstained

Answer 10

Negative stain (india ink)

Question 11

India ink results
Findings:
Encapsulated yeast cells - present

Answer 11

Positive for encapsulated yeast cell/s

Question 12

India ink results
Findings:
Encapsulated yeast cells - absent

Answer 12

No encapsulated yeast cell/s seen

Question 13

Direct examination of specimen for rapid detection of fungal elements

Answer 13

KOH Wet Mount

Question 14

Direct examination of specimen by using a

Answer 14

CLEARING AGENT (KOH Potassium Hydroxide)

Question 15

How many percent of KOH utilized for skin scrapings and other specimens

Answer 15

10% KOH (Potassium Hydroxide)

Question 16

How many percent of KOH utilized for tissue, nails, hair

Answer 16

20% KOH (Potassium Hydroxide)

Question 17

is a compound that stains the fungal cell wall, can be helpful in revealing fungal elements in wet mounts when examined under a fluorescence microscope.

Answer 17

Calcofluor White

Question 18

Not to use KOH reagent if is

Answer 18

old or already crystallized

Question 19

KOH Wet Mount - Results
Findings
Positive of Yeast cells and Hyphae

Answer 19

Reporting
Positive for yeast cells and hyphae

Question 20

KOH Wet Mount - Results
Findings:
Present of Yeast Cells, absent of hyphae

Answer 20

Positive for yeast cells

Question 21

KOH Wet Mount - Results
Findings:
Absent of Yeast Cells, present of hyphae

Answer 21

Reporting:
Positive for hyphae

Question 22

KOH Wet Mount - Results
Findings:
Absent of Yeast Cells, absent of hyphae

Answer 22

Reporting:
No fungal elements seen

Question 23

The polysaccharide capsule of Cryptococcus spp. exclude the ink particles giving a
—- around the organism
in a semi-opaque background

Answer 23

“clear halo”