9 Flashcards
The basic structural unit of molds
Hypha
Colony Color: SURFACE
Cottony
Velvety
Powdery
Texture
Cottony/floccose
Velvety/Suede
Powdery/Granular
kills any live microorganisms
Phenol
preserves fungal structures
Lactic Acid
stains the chitin present in the fungal cell wall
Cotton Blue
Microscopic methods for examination of fungi
- Tease mount technique
- Scotch tape method/Cellophane tape method
- Riddell Culture Method / Microculture method
I. Tease Mount Technique Disadvantage
Morphology is not intact.
Tease Mount Technique Advantage
Widely used method for staining and observing fungi
Cellophane Tape Mount Disadvantage
• Only superficial structures of fungi tend to stick to the tape.
• Rarely used technique because of inconvenience
Cellophane Tape Mount Advantage
Compared to Tease mount technique, morphology appears intact.
acropetal
youngest at the tip
basipetal
youngest at the base
Synchronous Arrangement of conidia
Example
Aureobasidium pullulans
Sympodial Arrangement of conidia example
Beauveria spp
Types of conidiogenous cells
• Annelide
• Phialide
• Thallic conidiogenesis
Swells at a tip
holothallic
Hyphal elements are fragment
holoarthric
The inner cells forms the conidia
enteroarthric
Thallic Conidiogenesis
holothallic
holoarthric
Eneteroarthic
Conidial head morphology of Aspergillus
biseriate
uniseriate
biseriate
Aspergillus niger
uniseriate
Aspergillus fumigatus
Phialidic
It is a vase like e.g. Phialophora verrucosa
Annelidic
There is a narrow septum to separate e.g. Scopulariopsis brevicaulis
Septations example
Amerocinidia
Didymoconidia
Phragmoconidia
Dictyoconidia
1- celled
ameroconidia
2-celled
didymoconidia
multi-celled (transverse only)
phragmoconidia
multicelled (transverse and longitudinal)
dictyoconidia