10 Flashcards
Diseases cause by fungi
Mycoses
SUPERFICIAL MYCOSES
Characteristics
Lacks immune response from host
• No living tissue invaded • asymptomatic
SUPERFICIAL MYCOSES
These are cosmetic fungal infections of the skin or hair shaft.
SUPERFICIAL MYCOSES Diseases
❏ Pityriasis versicolor
❏ Tinea nigra
❏ White piedra
❏ Black piedra
Also called Tinea versicolor
PITYRIASIS VERSICOLOR
PITYRIASIS VERSICOLOR Etiological agent
Malassezia furfur
A chronic, superficial fungal disease of the skin characterized by well-demarcated white, pink, fawn, or brownish scaly lesions.
PITYRIASIS VERSICOLOR
Basidiomycetous yeasts and form part of the normal skin flora
of humans and animals
Malassezia species
PITYRIASIS VERSICOLOR
o Laboratory diagnosis:
o Clinical Material o Skin scrapings
LABORATORY DIAGNOSIS culture of M. Furfur
Saboraud’s agar with olive oil or lanolin
o Stimulates growth by natural oils and other fatty substances
o Can grow on Sabouraud’s Dextrose Agar with cycloheximide and chloramphenicol (mycosel)
Specialized isolation medium containing glycerol-mono- oleate
Dixon Agar
Characterized by appearance, primarily on palms of the hand and occasionally the plantar and other surfaces of the skin as brown to black non scaly macules
TINEA NIGRA
TINEA NIGRA Etiologic agents
Hortaea werneckii
Hortaea werneckii example
Exophiala werneckii
Phaeoannellomyces werneckii
Cladosporium werneckii)
Clinical materials for the laboratory diagnosis of Tinea nigra
Skin scrappings
Direct microscopy of the laboratory diagnosis of Tinea Nigra
10% KOH (Potassium Hydroxide)
Direct Microscopy Examination (10%
KOH) of Tinea Nigra
- olive-brown, septate, multiple
branched hyphae and elongated budding cells - (+) ANNELIDE FORMATION
Culture for the laboratory diagnosis of Tinea Nigra
Saborauds dextrose agar
typical two-celled yeast cells producing annelloconidia (Tinea nigra)
LPCB mount:
Superficial infection of hair shaft
PIEDRA
PIEDRA 2 varieties
- white piedra - Trichosporon spp (asahi formerly beigelli)
- black Piedra - Piedraia hortae
Superficial fungal infection of the hair shaft
BLACK PIEDRA
BLACK PIEDRA Etiologic agent
Piedraia hortae
an ascomycetous fungus forming hard black nodules on the shafts of the scalp, beard,and pubic hair.
BLACK PIEDRA (Piedraia hortae)
BLACK PIEDRA
⮚Clinical Manifestation:
⮚Does not penetrate hair follicle
⮚Scalp hair are rough or sandy
⮚Infected hair: Hard black nodules on shaft
Black Piedra
LABORATORY DIAGNOSIS
o Clinical Material
Hair with hard black nodules
Black piedra DirectMicroscopy
LABORATORY DIAGNOSIS
o 20% KOH
o Darkly pigmented nodules
o Asci(ascospores)
Black Piedra Primary isolation medium
LABORATORY DIAGNOSIS
o Sabouraud’s dextrose agar
o Dark brown colonies
Fungal infection of the hair shaft with soft greyish-white nodules
WHITE PIEDRA
White piedra etiologic agent
Trichosporon spp.
Clinically significant Trichosporon spp.
Trichosporon asahii
⮚Trichosporon mucoides
⮚Trichosporon inkin
⮚Trichosporon cutaneum ⮚Trichosporon ovoides ⮚Trichosporon asteroides
These are fungal infections of the skin, hair and nails.
o Pathological changes and inflammatory response occur in the host
o symptomatic
CUTANEOUS MYCOSES
Diseases of CUTANEOUS MYCOSES
o Dermatophytosis (Tinea or ringworm)- caused by dermatophytes (Epidermophyton, Microsporum and Trichophyton)
o Candidiasisofskin,mucousmembranesandnails- caused by Candida spp.
Causes of dermatophytosis
dermatophytes (Epidermophyton, Microsporum and Trichophyton)
Candidiasisofskin,mucousmembranesandnails- caused by
Candida spp.
Also called Tinea or Ringworm
Disease of the nails, hair and skin caused by filamentous fungi called Dermatophytes
Dermatophytosis
Dermatophytosis Etiological agents:
Epidermophyton floccosum Microsporum spp. Trichophyton spp.
Skin and Nails (+)
Nails (-)
Microsporum
Skin and Nails (+)
Hair (-)
Epidermophyton
Skin, Hair, Nials (+)
Trichophyton
refers to dermatophytosis of the feet or toes. It is characterized by itchy and scaly rash
Tinea pedis
refers to dermatophytosis of the proximal medial thighs and buttocks.
• It manifests as a pruritic, scaled lesion or plaque that involves the fold between the scrotum and thigh.
Tinea cruris
dermatophytosis of the nail (onychomycosis)
-patches or pits on the surface of the nail
Tinea unguium
dermatophytosis of the glabrous skin. It’s usually an itchy, circular rash with clearer skin in the middle.
Tinea corporis-
refers to dermatophytosis of the scalp. It is characterized by itchy, scaly, bald patches on the head.
Tinea capitis
zoophilic dermatophyte (cats)
Microsporum canis
produces favus-like crusts or scutula and permanent hair loss.
Trichophyton schoenleinii
o A primary or secondary mycotic infection caused by members of the genus Candida.
o Clinical manifestations includes oropharyngeal candidiasis and cutaneous candidiasis
Candidiasis
LABORATORY DIAGNOSIS
o Direct Microscopy
CANDIDIASIS
KOH (10% or 20%)
LABORATORY DIAGNOSIS Clinical material
Skin scrapings, nail clippings and epilated hair
Laboratory diagnosis culture (candidiasis)
SDA
MYCOSEL
DERMATOPHYTE test medium. - selective medium for dermatophyte
Laboratory Identification of Dermatophyte macroscopic
- Surface color
- Reverse color
- Texture of growth
- RATE OF GROWTH
-Rapid grower
-Slow grower
Laboratory diagnosis of dermatophytes microscopic
Method:
1. Direct tease mount (LPCB)
2. Microculture (Ridell Technique)
identification is based on:
1. Microconidia
2. Macroconidia