Microscopic Examination of Urine - RBC, WBC, and Epithelial Cells Flashcards
Crenated RBCs are seen in urine that is:
Hypertonic
“Ghosts” or swollen RBCs are seen in urine that is:
Hypotonic
How many RBCs are seen in normal urine?
0-2/hpf
What are two reasons for a positive chem strip, but no visible RBCs in the urine?
- Blood is alkaline.
2. Hypotonic urine. Check specific gravity.
What are two reasons for a negative chem strip, but visible RBCs in urine?
- Ascorbic acid.
2. Check for yeast or calcium oxalate crystals.
RBCs in urine indicates:
- Patient is in early renal disease.
- Dysomorphic = diseased glomerulus.
- Can be a contaminant.
- Can be caused by smoking and excessive exercise.
RBCs can be mistaking for:
- WBCs.
- Yeast.
- Round from of calcium oxalate.
- Oil droplets.
- Air bubbles.
What is the physical appearance of neutrophils?
- Larger than red blood cells.
- Vary in size with tonicity.
- Grainy cytoplasm and segmented nuclei.
- Grayish single or clumps (report clumps).
What is the physical appearance of aging neutrophils?
- Nuclear lobes fuse.
- Swell and may lyse.
- “Glitter cells”.
- Cytoplasma forms granular blebs or filaments.
What is the total number of neutrophils in hpf?
0-5 or 0-8.
During physical examination of the urine, how will neutrophils make the urine look?
- Cloudy.
- Foul oder.
- macroscopic white button.
Why would a reagent strip test positive for neutrophils (WBC), but be negative under the microscope?
Lysis
Why would a reagent strip test negative for neutrophils (WBC), but be negative under the microscope?
- Other types of cells.
- Check test strip function.
- Not enough cells to cause reaction.
Neutrophils look like:
- Tubular epithelial cells.
- Red bleed cells.
- Trichomonads and fungi.
What is used to differentiate neutrophils from renal tubular epithelial cells?
- 2% acetic acid.
- 0.5% toluidine blue.