microscopes Flashcards
what is magnification?
makes objects bigger
what is resolution?
keeps the image clear
how does a light microscope work?
light passes though the specimen and into the magnifying lense.
magnification = x100-1500
what are the advantages of a light microscope?
cheap
small
easy to use
what are the disadvantages of a light microscope?
lower magnification
lower resolution
need a thin sample
how does a scanning electron microscope (SEM) work?
bean of electrons bounces of the surface of a sample.
magnification = x100,000
what are the advantages of a SEM microscope?
higher resolution
higher magnification
works fast
what are the disadvantage of a SEM microscope?
more expensive
training required
small risk of radiation exposure
how does a transmission electron microscope (TEM) work?
passes beams of electrons through the specimen to magnify it.
magnification = x500,000 - 1,000,000
what are the advantages of a TEM microscope?
best magnification
high quality
wife range of application
what are the disadvantages of a TEM microscope?
large
difficult to prepare specimen
black and white images
