Microscope Flashcards
What r the 2 types of Microscopes
Light microscope and electronic microscope
Name the 3 main thing a light microscope can do
View live specimens, magnification up to around ×2000, resolution is limited (0.2 micrometers)
Name 4 main things a electronic microscope can do
Electron is used instead if light, can’t view live specimen , much higher magnification(up to ×2,000,000, higher resolution (0.1 nanometers)
What’s magnification
How much large the image is compared to the actual object
What’s the definition for resolution
How clar and detailed an image is
What’s the equation for magnification
Image size÷real size
What’s image size measured in
Image size is measured in millimetres (convert to micrometer if necessary)
Real size refers to what
To the actual size of the object
Real size refers to what
To the actual size of the object
What does the eyepiece do?
Magnifies the image
What does the objective lens do
Further magnifies(low,medium,high)
What does the stage do
Holds the slide
What does the light source do?
Illuminates the sample
Name the 2 types of focus knobs
Coarse focus knob and fine focus knob
Wha is coarse focus knob used for?
For general focusng
What is fine focus knob used for
For detailed focusing
How to prepare a slide (practical)
●use a light microscope
●collect a thin sample(e.g onion epidermis or cheek cells)
●Add a drop of stain (e.g iodine for plant cells or methylene blue for animal cells
●Lower a cover slip gently to avoid air bubbles
●Place the slide on the stage and secure it.
What to do if u want to observe a specimen
●Set up the microscope on low power
●Place ur own prepared slide on the stage
●Use the coase focus knob to focus roughly, then the fine focus knob for detail
●Increase magnification if necessary, adjusting focus as you go.
What’s a stain
A stain is a chemical used to highlight different parts of cells under a microscope
What does I stand for
Image size
Wha does A stand for
Actual size
Wha does A stand for
Actual size
What does M stand for
Magnification
How do u find the image size
I=A×M
