Microscope Flashcards
In the 17th century, the amateur Dutch scientist ____ used one of the first microscopes to discover a whole new world of living organisms
Anton van Leeuwenhoek
Using a single lens, or ____ he observed tiny organisms in pond water and scrapings from his own teeth
Simple microscope
Discovered that larger organisms had small microscopic subunits that he called boxes or cells
Robert Hooke
The microscopes used in this course are ___ made of a set of lenses
Compound microscope
Each lens closest to your eye is an ____. It magnifies an image by the factor indicated on the ocular’s barrel =, usually 10x
Ocular lens or eyepiece
Holds the ocular in place
Body tube
At the bottom of the body tube is the ___. A turret-like circular mechanism rotates so that different lenses can be selected.
Revolving nosepiece
Each of the lens sets attached to the revolving nosepiece is an ____
Objective lens
is used for initial location of the specimen
4x (Scanning objective)
may also be used for initial location of the specimen. It is also used for observing specimens that do not need greater magnification
10x (Low-power objective)
Is used for specimens requiring greater magnification. This objective lens is called dry lens because it does not require the use of oil
40x (High-dry objective)
Is used for magnification of extremely small specimens, such as bacterial cells. It must be immersed in oil, so it is called the
100x (high-oil objective)
Is determined by multiplying the power of the ocular lens by the power of the objective lens in use
Total magnification
The specimen is usually mounted on a glass or plastic ___ that rests on stage
Microscopic slide
If the stage has an adjustable bracket that moves the slide around mechanically, the stage is called ___, if not, the slide is held by ___ and must be moved by hands
Mechanical stage, stage clips
Below the stage is a high-intensity ___. Light rays from the lamp travel through a hole in the stage, through the specimen mounted in a slide, and then through the objective and ocular to the eye
Lamp
A ___ is a lens that concentrates lights, may be found between the lamp and the stage
Condenser
Light intensity may also be adjusted by adjusting the ___
Diaphragm
A ____ is a rotating disk with holes of different diameters.
Disk diaphragm
An ____ is made of overlapping slivers of metal in a pattern resembling the iris flower, as does the iris of the human eye.
It can dilate or constrict its opening.
Iris diaphragm
The entire upper assembly of the microscope is held in an upright position by a bar called the ___.
Arm
The scope is supported by a square or horseshoe-shaped ___. The arm may be connected to the base by a ___, which allows the upper assembly to move into a more comfortable viewing position.
Base, pivot
The ___ changes the distance very little
fine-focus knob
the _____ changes the distance greatly.
coarse-focus knob
Before you turn on the microscope, make sure that the light intensity dial is on the ____. If the light intensity dial is on a high setting, the sudden voltage surge may burn out the lamp.
lowest setting
TRUE or FALSE
Adjust the coarse focus when using the high-power lens
False
A ___ slide is a slide on which a wet specimen is placed, then covered with a coverslip.
wet-mount
___ are used to make a specimen, or some of its parts, more visible.
Stains