MICROBIOLOGY QUIZ Basic Equipment and tools used in micro bio lab

Question 1

Refers to various tools, equipment, and instruments used in scientific laboratories to carry out experiments and research

Answer 1

Laboratory Apparatus

Question 2

What are the 4 different tools that has different categorized functions depending on its use

Answer 2

1. Used for Sterilization
2. Used for Preparatory of Culture Media
3. Used for Culture and ID of Microbes
4. Other Glassware and Apparatus that are used

Question 3

Sterilizes items by heating them with steam to a very high temperature (121 degrees Celsius or 15 p.si)

Answer 3

Autoclave

Question 4

Used to Dry heat sterilization

Answer 4

Hot Air Oven

Question 6

Produces gas flame which used for heating sterilization (inoculation loop, the tip of forceps, and spatula)

Answer 6

Bunsen Burner

Question 7

*Creates a sterile zone
*Used to flame the mouths of tubes and flask before and after use and remove gas bubbles formed during pouring media

Answer 7

Bunsen Burner

Question 8

Measure the Precision in determining the mass of solids, liquids, powders, and granular substances.

Answer 8

Analytical Balance

Question 9

What are the 3 equipments used for sterilization

Answer 9

1 Autoclave
2 Hot Air Oven
3 Bunsen Burner

Question 10

An electronic device and uses the principle of magnetic force restoration, offering readability up to 0.0001g

Answer 10

Analytical Balance

Question 11

Top loading balance precision is only 0.01g

Answer 11

Analytical Balance`

Question 12

Used as a primary barrier against exposure to infectious biological agents

Answer 12

Biological Safety Cabinets

Question 13

Principal device used for containment while dealing with microbiological samples

Answer 13

Biological Safety Cabinets

Question 14

Provides heat to solutions and materials uniformly.

Answer 14

Hot Plate

Question 15

Much safer than bunsen burner

Answer 15

hot plate

Question 16

In the microbiology laboratories, it serves their purpose in making agar and media biochemical media

Answer 16

Hot plate

Question 17

Used to dissolve substance easily and quickly.

Answer 17

Magnetic Stirrer and Stir Bar

Question 18

It is a small teflon coated magnet. What is this called?

Answer 18

stirring bar

Question 19

What are the tools and equipment used for preparation of culture media

Answer 19

1 Analytical Balance
2 Biological Safety Cabinet (BSC)
3 Hot Plate
4 Magnetic Stirrer
5 Stir Bar

Question 20

A warm cabinet that you can set the temperature optimal for the growth and cultivation of the microbes

Answer 20

Incubator

Question 21

what is the good temperature for most Bacteria?

Answer 21

35 degrees celsius

Question 22

A good temperature for most fungi?

Answer 22

25 degrees celsius

Question 23

Primarily used to measure the acidity of pharmaceutical chemicals, cultures, soil, and water treatment plants

Answer 23

pH Meter

Question 24

1.pH1.0 to pH 6.9
2. pH 7.0
3. pH 7.1 to 14.0

Answer 24

1. Acidic (Vinegar, Tomato, Urine)
2. Neutral (Water)
3. Alkaline (Sea Water, Ammonia Sol.)

Question 25

Used to study proper and enlarged and well-defined images of object that are too small to be observed with the naked eye

Answer 25

Microscope

Question 26

Used for through mixing of liquids in test tubes.

Answer 26

Vortex Mixer

Question 27

Has a rotor, whose speed can be controlled. On the tip of the rotor is a foam- rubber top

Answer 27

Vortex Mixer

Question 28

Particularly helpful during serial dilution in enumeration of bacteria, which needs homogenous suspension of bacteria cells

Answer 28

Vortex Mixer

Question 29

What are the EQUIPMENTS used for preparation of Culture and ID microbes

Answer 29

1. incubator 2. pH Meter 3. Microscope 4. Vortex Mixer 5. Colony Counter 6. Spectrophotometer 7.Refrigirator

Question 30

It is a machine that automatically counts colonies in the agar plate in the microbiology laboratory.

Answer 30

Colony Counter

Question 31

It uses fluorescent lables or the contrast between light and dark areas on the plate to measure the colonies.

Answer 31

Colony Counter

Question 32

An optical instrument for measuring the intensity of light in relation to wavelength

Answer 32

Spectrophotometer

Question 33

Applied for the measurement of the substance concentration of protein, nucleic acids, bacterial growth, and enzymatic reactions.

Answer 33

Spectrophotometer

Question 34

Provides a low temperature environment

Answer 34

Refrigirator

Question 35

it Preserves those materials and stock culture that may decompose and destroy when kept at room temperature

Answer 35

Refrigirator

Question 36

Used to store blood, cultures, media prepared, serums, antibiotics, and other chemicals.

Answer 36

Refrigirator

Question 37

A glass container that has a flat bottom

Answer 37

Beaker

Question 38

it is used for holding or storing liquids

Answer 38

Beaker

Question 39

Flask that is a cone shaped with a flat round bottom and cylindrical neck

Answer 39

Erlenmeyer flask

Question 40

what is the purpose of erlenmeyer flask

Answer 40

Prepare media

Question 41

an instrument made of glass or plastic used to measure the volume of different liquids

Answer 41

Graduated Cylinder

Question 42

Pislita lang about cylinder

Answer 42

Measuring cylinders are graduated, measuring the volume in ml (milliliter)

Question 43

a piece of laboratory equipment used to mix chemicals and liquids

Answer 43

Glass Rods

Question 44

Pislita lang about glass rods

Answer 44

Bent glass rods or L shaped Glass rods - spread dilute specimens over the agar surface. Straight glass rods - used to stir agar and solution

Question 45

Used in staining and observing under the microscope

Answer 45

Glass slides

Question 46

Apart from slides it can also be used as coverslips for wet mount preparation for observing parasites

Answer 46

Glass slides

Question 47

The glassware that helps deliver a specific quantity of fluid from one container to another

Answer 47

Glass Pipette

Question 48

A piece of calibrated glass tube marked, and a sample is drawn in a small capillary used with pipette bulb or aspirator

Answer 48

Glass Pipette

Question 49

Is a common yet essential laboratory instrument used to transfer volumes of liquid accurately and precisely in the microliter range

Answer 49

Micro pipette

Question 50

It helps grow microorganisms by providing an artificial environment, preparing stock culture, and performing various biochemical tests.

Answer 50

Test Tube

Question 51

Uses borosilicate glass, and the open end is plain without lips

Answer 51

Test Tube

Question 52

They have cotton plugs as stopper, but using metal or plastic caps is possible

Answer 52

Test Tube

Question 53

Transfer microbial growth from one container to another without introducing any unwanted organisms

Answer 53

Inoculating loop and needle

Question 54

Is a round concave glass dish

Answer 54

Watch glass

Question 55

It can be employed for weighing solids as a lid for flasks and beakers

Answer 55

Watch Glass

Question 56

The other miscellaneous Press em

Answer 56

1. Spatula – used in laboratories to mix, spread, or transfer materials. 2. Cotton Plug – used to seal laboratory glassware, preventing contamination. 3. Cotton Swab – used for applying or collecting substances in a precise manner. 4. Parafilm – used to cover and protect laboratory containers to prevent contamination and evaporation.

Question 57

What are the glassware and apparatus used in the microbiology lab

Answer 57

1. Beaker 2. Erlenmeyer Flask 3. Graduated Cylinder 4. Stirring Rods 5. Glass Slides 6. Glass Pipette 7. Micropipette 8.Test Tube 9. Inoculating loop and needle 10. Watch Glass