MICROBIOLOGY QUIZ Basic Equipment and tools used in micro bio lab Flashcards

1
Q

Refers to various tools, equipment, and instruments used in scientific laboratories to carry out experiments and research

A

Laboratory Apparatus

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2
Q

What are the 4 different tools that has different categorized functions depending on its use

A
  1. Used for Sterilization
  2. Used for Preparatory of Culture Media
  3. Used for Culture and ID of Microbes
  4. Other Glassware and Apparatus that are used
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3
Q

Sterilizes items by heating them with steam to a very high temperature (121 degrees Celsius or 15 p.si)

A

Autoclave

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4
Q

Used to Dry heat sterilization

A

Hot Air Oven

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5
Q
A
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6
Q

Produces gas flame which used for heating sterilization (inoculation loop, the tip of forceps, and spatula)

A

Bunsen Burner

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7
Q

*Creates a sterile zone
*Used to flame the mouths of tubes and flask before and after use and remove gas bubbles formed during pouring media

A

Bunsen Burner

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8
Q

Measure the Precision in determining the mass of solids, liquids, powders, and granular substances.

A

Analytical Balance

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9
Q

What are the 3 equipments used for sterilization

A

1 Autoclave
2 Hot Air Oven
3 Bunsen Burner

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10
Q

An electronic device and uses the principle of magnetic force restoration, offering readability up to 0.0001g

A

Analytical Balance

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11
Q

Top loading balance precision is only 0.01g

A

Analytical Balance`

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12
Q

Used as a primary barrier against exposure to infectious biological agents

A

Biological Safety Cabinets

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13
Q

Principal device used for containment while dealing with microbiological samples

A

Biological Safety Cabinets

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14
Q

Provides heat to solutions and materials uniformly.

A

Hot Plate

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15
Q

Much safer than bunsen burner

A

hot plate

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16
Q

In the microbiology laboratories, it serves their purpose in making agar and media biochemical media

A

Hot plate

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17
Q

Used to dissolve substance easily and quickly.

A

Magnetic Stirrer and Stir Bar

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18
Q

It is a small teflon coated magnet. What is this called?

A

stirring bar

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19
Q

What are the tools and equipment used for preparation of culture media

A

1 Analytical Balance
2 Biological Safety Cabinet (BSC)
3 Hot Plate
4 Magnetic Stirrer
5 Stir Bar

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20
Q

A warm cabinet that you can set the temperature optimal for the growth and cultivation of the microbes

A

Incubator

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21
Q

what is the good temperature for most Bacteria?

A

35 degrees celsius

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22
Q

A good temperature for most fungi?

A

25 degrees celsius

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23
Q

Primarily used to measure the acidity of pharmaceutical chemicals, cultures, soil, and water treatment plants

A

pH Meter

24
Q

1.pH1.0 to pH 6.9
2. pH 7.0
3. pH 7.1 to 14.0

A
  1. Acidic (Vinegar, Tomato, Urine)
  2. Neutral (Water)
  3. Alkaline (Sea Water, Ammonia Sol.)
25
Q

Used to study proper and enlarged and well-defined images of object that are too small to be observed with the naked eye

A

Microscope

26
Q

Used for through mixing of liquids in test tubes.

A

Vortex Mixer

27
Q

Has a rotor, whose speed can be controlled. On the tip of the rotor is a foam- rubber top

A

Vortex Mixer

28
Q

Particularly helpful during serial dilution in enumeration of bacteria, which needs homogenous suspension of bacteria cells

A

Vortex Mixer

29
Q

What are the EQUIPMENTS used for preparation of Culture and ID microbes

A
  1. incubator
  2. pH Meter
  3. Microscope
  4. Vortex Mixer
  5. Colony Counter
  6. Spectrophotometer
    7.Refrigirator
30
Q

It is a machine that automatically counts colonies in the agar plate in the microbiology laboratory.

A

Colony Counter

31
Q

It uses fluorescent lables or the contrast between light and dark areas on the plate to measure the colonies.

A

Colony Counter

32
Q

An optical instrument for measuring the intensity of light in relation to wavelength

A

Spectrophotometer

33
Q

Applied for the measurement of the substance concentration of protein, nucleic acids, bacterial growth, and enzymatic reactions.

A

Spectrophotometer

34
Q

Provides a low temperature environment

A

Refrigirator

35
Q

it Preserves those materials and stock culture that may decompose and destroy when kept at room temperature

A

Refrigirator

36
Q

Used to store blood, cultures, media prepared, serums, antibiotics, and other chemicals.

A

Refrigirator

37
Q

A glass container that has a flat bottom

A

Beaker

38
Q

it is used for holding or storing liquids

A

Beaker

39
Q

Flask that is a cone shaped with a flat round bottom and cylindrical neck

A

Erlenmeyer flask

40
Q

what is the purpose of erlenmeyer flask

A

Prepare media

41
Q

an instrument made of glass or plastic used to measure the volume of different liquids

A

Graduated Cylinder

42
Q

Pislita lang about cylinder

A

Measuring cylinders are graduated, measuring the volume in ml (milliliter)

43
Q

a piece of laboratory equipment used to mix chemicals and liquids

A

Glass Rods

44
Q

Pislita lang about glass rods

A

Bent glass rods or L shaped Glass rods - spread dilute specimens over the agar surface.

Straight glass rods - used to stir agar and solution

45
Q

Used in staining and observing under the microscope

A

Glass slides

46
Q

Apart from slides it can also be used as coverslips for wet mount preparation for observing parasites

A

Glass slides

47
Q

The glassware that helps deliver a specific quantity of fluid from one container to another

A

Glass Pipette

48
Q

A piece of calibrated glass tube marked, and a sample is drawn in a small capillary
used with pipette bulb or aspirator

A

Glass Pipette

49
Q

Is a common yet essential laboratory instrument used to transfer volumes of liquid accurately and precisely in the microliter range

A

Micro pipette

50
Q

It helps grow microorganisms by providing an artificial environment, preparing stock culture, and performing various biochemical tests.

A

Test Tube

51
Q

Uses borosilicate glass, and the open end is plain without lips

A

Test Tube

52
Q

They have cotton plugs as stopper, but using metal or plastic caps is possible

A

Test Tube

53
Q

Transfer microbial growth from one container to another without introducing any unwanted organisms

A

Inoculating loop and needle

54
Q

Is a round concave glass dish

A

Watch glass

55
Q

It can be employed for weighing solids as a lid for flasks and beakers

A

Watch Glass

56
Q

The other miscellaneous
Press em

A
  1. Spatula – used in laboratories to mix, spread, or transfer
    materials.
  2. Cotton Plug – used to seal laboratory glassware, preventing
    contamination.
  3. Cotton Swab – used for applying or collecting substances in a
    precise manner.
  4. Parafilm – used to cover and protect laboratory containers to
    prevent contamination and evaporation.
57
Q

What are the glassware and apparatus used in the microbiology lab

A
  1. Beaker
  2. Erlenmeyer Flask
  3. Graduated Cylinder
  4. Stirring Rods
  5. Glass Slides
  6. Glass Pipette
  7. Micropipette
    8.Test Tube
  8. Inoculating loop and needle
  9. Watch Glass