MICROBIOLOGY LAB EX 3 QUIZ CULTURE MEDIA

Question 1

Survival growth of microorganisms depend on?

Answer 1

available nutrients
favorable growth environment

Question 2

The nutrient preparation used for culturing microorganism is called?

Answer 2

Media

Question 3

What are the three physical forms of media?

Answer 3

liquid or broth media
semi solid media
solid media

Question 4

The major difference among the media is the presence of?

Answer 4

solidifying agent (agar)

Question 5

What is the solidifying agent?

Answer 5

Agar

Question 6

can be used to propagate large numbers of microorganisms in fermentation studies?

Answer 6

nutrient broth media

Question 7

Are used in determining bacterial mobility in promoting anaerobic growth?

Answer 7

Semisolid media

Question 8

used for surface growth in order to observe colon appearance and for pure culture isolation?

Answer 8

solid media
blood agar
nutrient agar

Question 9

What are types of Liquid media?

Answer 9

Nutrient broth

Question 10

What are types of Solid media?

Answer 10

nutrient agar or blood agar

Question 11

It is usually poured into a test tube?

Answer 11

Liquid media

Question 12

Can be poured in a test tube or plate?

Answer 12

A semisolid media in a liquified state

Question 13

The medium that is allowed to harden in a slanted position is called?

Answer 13

agar slant

Question 14

If the medium is in a upright position it is designated as?

Answer 14

agar deep tube or butt

Question 15

If the agar is poured into a petri plate, the plate is designated as?

Answer 15

agar plate

Question 16

A process of rendering a medium or material free of all forms of life?

Answer 16

Sterilization

Question 17

The three basic ways of sterilizing media and supplies

Answer 17

autoclaving
dry heat sterilization
Ultra Violet (UV) radiation

Question 18

The most useful approach of sterilization is?

Answer 18

autoclaving

Question 19

items are sterilized by exposure to steam at 121 degrees Celsius and 15 lbs. of pressure for 15 minutes or longer depending on the item?

Answer 19

autoclaving

Question 20

endospores cannot survive longer than about 12 to 13 minutes?

Answer 20

Autoclaving

Question 21

This method is rapid and dependable?

Answer 21

Autoclaving

Question 22

What are the Materials in EXERCISE 3?

Answer 22

Distilled Water
Magnetic Stirrer with magnetic bar
Nutrient Broth Powder
Nutrient Agar Powder
Slant rack and test tube rack
Graduated cylinder
Hot Plates
Stirring Rod
Erlenmeyer flask
Watch glass
Beam balance
Spatula
Sterile test tubes and petri plates
Cotton plug
Aluminum Foil

Question 23

How many grams do you need to dissolve in Nutrient broth?

Answer 23

8 grams

Question 24

how many ml of distilled water is in a Erlenmeyer flask?

Answer 24

1000 ml

Question 25

Mix and heat over the magnetic stirrer until the medium becomes?

Answer 25

clear or transparent

Question 26

How much ml of the medium do you dispense of Nutrient Broth go into the test tubes?

Answer 26

7-10 ml

Question 27

What is the 3rd step in Nutrient broth?

Answer 27

Stop the test tubes completely

Question 28

In Nutrient Broth what do you use for sterilization?

Answer 28

autoclave at 15psi

Question 29

How many degrees does sterilization and how many minutes? in the Nutrient Broth need to sterilize?

Answer 29

121 degrees celsius for 15 minutes

Question 30

How many grams of NA powder does it is being put in a 1000 ml distilled water in a erlenmeyer flask

Answer 30

28 grams

Question 31

how many ml of distilled water does NA need in a erlenmeyer flask?

Answer 31

1000 ml

Question 32

In Nutrient Agar what do you use for sterilization?

Answer 32

autoclave at 15 psi, 121 degrees celsius for 15 minutes

Question 33

In the preparation of Nutrient Agar Plate what kinds of apparatus are used?

Answer 33

Petri dishes inoculating hood

Question 34

In the preparation of Nutrient Agar slant what kinds of apparatus are used?

Answer 34

sterile test tubes pipette or syringe sterile cotton plug or screw cap slant rack

Question 35

In the preparation of Nutrient Agar Deep what kinds of apparatus are used?

Answer 35

sterile test tubes pipette or syringe sterile cotton plug test tube rack

Question 36

How much ml of medium is dispensed into the sterile test tube of Nutrient Agar Slant and Nutrient Agar Deep?

Answer 36

7-10

Question 37

How much ml is needed to be dispensed in Nutrient Agar Plate?

Answer 37

10 - 15 ml

Question 38

how many minutes does Nutrient Agar need to elapse before covering the dish to avoid contamination?

Answer 38

5 - 10 minutes

Question 39

Why does Nutrient Agar Plate need to be covered?

Answer 39

to avoid contamination

Question 40

What if the agar is not ready to be inoculated yet what would you do?

Answer 40

Refrigerate the agar in an upside down position

Question 41

What if the medium is ready to be inoculated and is taken from the refrigerator what will you do?

Answer 41

Allow atleast 10-20 minutes to elapse for the medium at room tempretaure before inoculation.

Question 42

Media may also be dispensed in to the test tube, plugged with?

Answer 42

cotton or screw cap and autoclaved