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FlashCards for Quizes PRELIMS > MICROBIOLOGY LAB EX 3 QUIZ CULTURE MEDIA > Flashcards

MICROBIOLOGY LAB EX 3 QUIZ CULTURE MEDIA Flashcards

1
Q

Survival growth of microorganisms depend on?

A

available nutrients
favorable growth environment

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2
Q

The nutrient preparation used for culturing microorganism is called?

A

Media

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3
Q

What are the three physical forms of media?

A

liquid or broth media
semi solid media
solid media

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4
Q

The major difference among the media is the presence of?

A

solidifying agent (agar)

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5
Q

What is the solidifying agent?

A

Agar

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6
Q

can be used to propagate large numbers of microorganisms in fermentation studies?

A

nutrient broth media

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7
Q

Are used in determining bacterial mobility in promoting anaerobic growth?

A

Semisolid media

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8
Q

used for surface growth in order to observe colon appearance and for pure culture isolation?

A

solid media
blood agar
nutrient agar

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9
Q

What are types of Liquid media?

A

Nutrient broth

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10
Q

What are types of Solid media?

A

nutrient agar or blood agar

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11
Q

It is usually poured into a test tube?

A

Liquid media

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12
Q

Can be poured in a test tube or plate?

A

A semisolid media in a liquified state

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13
Q

The medium that is allowed to harden in a slanted position is called?

A

agar slant

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14
Q

If the medium is in a upright position it is designated as?

A

agar deep tube or butt

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15
Q

If the agar is poured into a petri plate, the plate is designated as?

A

agar plate

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16
Q

A process of rendering a medium or material free of all forms of life?

A

Sterilization

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17
Q

The three basic ways of sterilizing media and supplies

A

autoclaving
dry heat sterilization
Ultra Violet (UV) radiation

18
Q

The most useful approach of sterilization is?

A

autoclaving

19
Q

items are sterilized by exposure to steam at 121 degrees Celsius and 15 lbs. of pressure for 15 minutes or longer depending on the item?

A

autoclaving

20
Q

endospores cannot survive longer than about 12 to 13 minutes?

A

Autoclaving

21
Q

This method is rapid and dependable?

A

Autoclaving

22
Q

What are the Materials in EXERCISE 3?

A

Distilled Water
Magnetic Stirrer with magnetic bar
Nutrient Broth Powder
Nutrient Agar Powder
Slant rack and test tube rack
Graduated cylinder
Hot Plates
Stirring Rod
Erlenmeyer flask
Watch glass
Beam balance
Spatula
Sterile test tubes and petri plates
Cotton plug
Aluminum Foil

23
Q

How many grams do you need to dissolve in Nutrient broth?

A

8 grams

24
Q

how many ml of distilled water is in a Erlenmeyer flask?

A

1000 ml

25
Q

Mix and heat over the magnetic stirrer until the medium becomes?

A

clear or transparent

26
Q

How much ml of the medium do you dispense of Nutrient Broth go into the test tubes?

A

7-10 ml

27
Q

What is the 3rd step in Nutrient broth?

A

Stop the test tubes completely

28
Q

In Nutrient Broth what do you use for sterilization?

A

autoclave at 15psi

29
Q

How many degrees does sterilization and how many minutes? in the Nutrient Broth need to sterilize?

A

121 degrees celsius for 15 minutes

30
Q

How many grams of NA powder does it is being put in a 1000 ml distilled water in a erlenmeyer flask

A

28 grams

31
Q

how many ml of distilled water does NA need in a erlenmeyer flask?

A

1000 ml

32
Q

In Nutrient Agar what do you use for sterilization?

A

autoclave at 15 psi, 121 degrees celsius for 15 minutes

33
Q

In the preparation of Nutrient Agar Plate what kinds of apparatus are used?

A

Petri dishes
inoculating hood

34
Q

In the preparation of Nutrient Agar slant what kinds of apparatus are used?

A

sterile test tubes
pipette or syringe
sterile cotton plug or screw cap
slant rack

35
Q

In the preparation of Nutrient Agar Deep what kinds of apparatus are used?

A

sterile test tubes
pipette or syringe
sterile cotton plug
test tube rack

36
Q

How much ml of medium is dispensed into the sterile test tube of Nutrient Agar Slant and Nutrient Agar Deep?

A

7-10

37
Q

How much ml is needed to be dispensed in Nutrient Agar Plate?

A

10 - 15 ml

38
Q

how many minutes does Nutrient Agar need to elapse before covering the dish to avoid contamination?

A

5 - 10 minutes

39
Q

Why does Nutrient Agar Plate need to be covered?

A

to avoid contamination

40
Q

What if the agar is not ready to be inoculated yet what would you do?

A

Refrigerate the agar in an upside down position

41
Q

What if the medium is ready to be inoculated and is taken from the refrigerator what will you do?

A

Allow atleast 10-20 minutes to elapse for the medium at room tempretaure before inoculation.

42
Q

Media may also be dispensed in to the test tube, plugged with?

A

cotton or screw cap and autoclaved

FlashCards for Quizes PRELIMS (14 decks)

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