MICRO BIO LAB QUIZ 4 Flashcards

1
Q

These are transferred from one culture medium to another by subculturing

A

Microorganisms

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2
Q

Using a specific procedure and aseptic technique

A

Subculturing

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3
Q

it is inoculated or introduced into various forms of culture media in order to keep them alive and to study growth

A

Bacteria

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4
Q

it must be done without introducing unwanted microbes or contaminants into the media

A

inoculation

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5
Q

it is used in microbiology to exclude contaminants

A

Aseptic techniques

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6
Q

media are sterilized or rendered free from all forms of life prior to use

A

all cultures

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7
Q

it is usually accomplished using autoclave during the media preparation?

A

Sterilization

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8
Q

what are the containers used in culture media?

A

test tubes and petri plates

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9
Q

it should not be opened or left opened until the culture media is ready for inoculation test

A

test tubes and petri plates

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10
Q

Transfer and inoculation are usually performed with?

A

sterile, heat resistant, non-corroding nichrome wire

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11
Q

what is attached to an insulated handle?

A

non-corroding nichrome wire

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12
Q

when the end of the wire is bent into a loop it is called?

A

inoculating loop

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13
Q

when the wire is straight it is called?

A

inoculating needle

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14
Q

what are the special purposes for transferring culture? depending on the form of the medium and technique

A

sterile cotton swab
pipette
glass rod
syringe

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15
Q

what ate the materials

A

inoculating loops
inoculating needles
inoculum
bacterial culture
alcohol lamp
incubator
nutrient agar plates
agar slants
agar deep
nutrient broths

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16
Q

what are the samples of inoculum?

A

sample from soil, water, feces, urine, sputum

17
Q

What are the kinds of bacterial culture used in this experiement?

A

Staphylococcus aureus and Escherichia coli

18
Q

how many degrees Celsius must the alcohol lamp be set

A

37 degrees Celsius

19
Q

In simple streak how many hours must the petri dish be incubated?

A

24-48 hours at 37 degrees Celsius

20
Q

in Incubation of inoculated culture media, how many hours must the agar plate and agar slant and deep be inoculated?

A

18-24 hours

21
Q

when we say agar plate media what must be the position?

A

upside down?

22
Q

when we say agar slant and deep what must be the position?

A

upright position on the test tube rack

23
Q

What is the title of the experiment?

A

Inoculation of culture media: Aseptic Transfer Technique