microbiology quiz 13 Flashcards
what does API stand for
The Analytical profile index
what is an Analytical profile index
a classification of bacteria based on
biochemical tests, allowing fast identification.
why was API system made
his system is developed for the quick identification of clinically relevant bacteria. Because of this, only known bacteria can be identified.
downside of API
only known bacteria can be identified.
when was API invented and by who
1970s in the United States by Pierre Janin of Analytab
Products Inc.
what does API20E stand for
API
* 20 = 20 tests
* E = Enterobacteriaceae
purpose of API20E
Conventional tests and allows the identification of a limited number of Gram-negative Enterobacteriaceae
what do you have to do before doing an API20E test
You have to confirm that the culture is of Enterobacteriaceae > oxidase test
how do you confirm that the culture is of Enterobacteriaceae
oxidase test
how many identification systems are there
Presently there are 15 separate identification systems
what type of cells are covered by identification test
practically all known pathogenic bacteria and yeasts.
how many species have been cataloged through tests
Over 600 different species have been cataloged. The different strips are tailored to certain groups of microbes.
API 10 S is for
simplified identification of Gram (-) rods
API Candida is for
identification of yeasts found in clinical infections
API 20 C AUX is for identification of
identification of yeasts
API Staph is for identification of
staphylococci and micrococci
what are API tests done on
plastic strips
Each test in API is performed in a
cupule containing a dehydrated substrate.
how many mL of water on incubation tray
5mL
5mL of what solution is an isolated colony put in
sterile saline,
After ____ hours examine the glucose test
18
what type of pipette is used
pasteur
after 18 hrs if tests are positive add what
TDA, VP, IND)
if after 18 hrs its negative and there are less then ___ other positive tests (those not requiring addition of reagents) continue incubation for further ______
hours before adding reagents
18-24
A ____-digit number is obtained by adding up the positive tests of each of the seven groups. This number
is then located in the profile index to identify the organism.
7
The tests are divided into ___ groups of three each.
* a value of __ is assigned to the first test in the group if it is positive.
* a value of __ is assigned to the second test in the group if it is positive.
* a value of __ is assigned to the third test in the group if it is positive.
* a negative test in any position gets a score of _.
7
1
2
4
0
what gram type is enterobacteriaceae
negative
