Microbio quiz mic Flashcards
Kirby-Bauer antimicrobial susceptibility test provides important information regarding the .
relative susceptibility or resistance of an organism to an antibiotic
what does the kirby baur test not provide
it does not provide information on a
level of antibiotic required to inhibit the growth of that microorganism
The minimum inhibitory
concentration (MIC) is the
lowest concentration of antibiotic that is still able to inhibit the growth of a
particular organism
The MIC should be differentiated from the
MBC
MBC
minimum bactericidal
concentration; the concentration of antibiotic required to kill an organism
Determination of the MIC is advantageous as it
indicates the concentration of antibiotic that should be achieved to inhibit the growth of organisms
By knowing the MIC and the levels of antibiotic that can be
achieved in body fluids the appropriate ______
dose of antibiotic can be prescribed
. In general, a concentration
of ___ times the MIC is used to ensure the successful treatment of an infection.
10
The antibiotics you use will be based on the results obtained from
the Kirby-Bauer assay. (select 2)
what are some antibiotics we will use
ampicilin, tetracyclin, erythromycin
how are 2 fgold dilutions made
add 2.5 ml of antibiotic dilution
to 2.5 ml of sterile saline
how mamny wells are there
2 rows of 6
which has the lowest concentration and what has the highst concentration
A6 has the lowest concentration
a1 has the highest concentration
add how many ml of TSA to wells
.8mL
dilute the cultures by how much
1:100 add.1mL of culture to 9.9mL sterile saline
