microbiology quiz 12

Question 1

Antibiotic=

Answer 1

against life

Question 2

Alexander Fleming accidentally discovered

Answer 2

penecilin

Question 3

Antibiotic resistance is the ability of an organism to grow in the presence of a

Answer 3

specific antibiotic/s.

Question 4

Commonly used in the lab for
determination of antibiotic
susceptibility/resistance.

Answer 4

Mueller-Hinton agar

Question 5

Mueller-Hinton agar contains

Answer 5

beef infusion, casein
hydrolysate, starch, neutral pH

Question 6

what type of agar is non differential and non selective

Answer 6

Mueller-Hinton agar

Question 7

loose agar allows

Answer 7

diffusion of antibiotics

Question 8

starch does what in Mueller-Hinton agar

Answer 8

abrorbs toxins produced by bacteria

Question 9

what type of culture does Mueller-Hinton agar use

Answer 9

lawn

Question 10

MIC

Answer 10

the lowest concentration of an antibacterial agent expressed in mg/L
(μg/mL) needed to completely prevents visible growth of the test strain of an
organism

Question 11

MBC

Answer 11

t is the lowest concentration of an
antibacterial agent required to kill a
particular bacterium

Question 12

MBC(minimum bacterixidal concentration) P.I.D

Answer 12

Perform, incubate, determine

Question 13

Perform in Minimum bactericidal concentration

Answer 13

perform a streak from MIC without growth for each organism/antibiotic combo

Question 14

incubate Minimum bactericidal concentration

Answer 14

incubate at 37 degrees o/n. in inverted position for 18-24 hrs

Question 15

determine in Minimum bactericidal concentration

Answer 15

determine which concentration is required to kill all.bacteria

Question 16

kirby bauer test principle

Answer 16

diffusion of antibiotics from a disk placed on an agar plate inoculated with bacteria; the size of the “zone of inhibition” around the disk indicates the bacteria’s susceptibility to the antibiotic