Microbiology - Aseptic Technique

Question 1

Why is aseptic technique important in culturing bacteria?

Answer 1

-Keep apparatus and equipment free from micro organisms
-Prevent cost contamination of other bacteria to get into petri dish
-prevent cultured bacteria to get into environment

Question 2

How to use aseptic before experiment

Answer 2

Area - wipe surface, wear glove, wash hands with soap or alcohol, Bunsen on full blast for ‘clean’ air

Media (petri dish) - autoclave

Reusable equipment - inoculating loop (things that scoop up bacteria from petri or environment) heated with Bunsen until red and cooled, glass wipe or autoclave

Single use plastic with equipment inside - radiation

Question 3

How to do aseptic during experiment

Answer 3

Media (petri) - lift open lid just enough to wipe it with bacteria (prevent air born bacteria)

Inoculating loop - immediately go over Bunsen (kill bacteria after scooping)

Question 4

How to do aseptic after culturing

Answer 4

-wipe down surfaces
-Remnant (used Petri dish) - seal lid with tape, prevent anaerobes since allow oxygen in (most anaerobes are human pathogens)
+under 25 degree (prevent human pathogens)

Remnant (petri used after cultured) - auto clave and bin

Question 5

Autoclave

Answer 5

-A seal container that allow glass or metal equipment to be heated at 121 degree and under pressure to destroy any bacteria (including those resistant to boiling)

-disposable equipment (petri dish) sealed inside autoclavable plastic (high temp resist) and be binned