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microbiology > microbiology 4 > Flashcards

microbiology 4 Flashcards

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Biology 101 flashcards
1
Q

tiny ruler within the eyepiece of a microscope that measures sizes of microbes

A

OCULAR MICROMETER

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2
Q

optical instrument that is used to observe tiny objects, often subjects that cannot be seen by our naked eyes.

A

MICROSCOPE

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3
Q

Types of Microscope:

A

SIMPLE MICROSCOPE
COMPOUND MICROSCOPE

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4
Q

defined as a microscope containing only one
magnifying lens.

A

SIMPLE MICROSCOPE

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4
Q

microscope that contains more than one magnifying lens

A

COMPOUND MICROSCOPE

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5
Q

these are photos taken through the lens system of compound microscopes

A

PHOTOMICROGRAPHS

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5
Q

contains two magnifying lens systems. Uses light as source of illumination

A

COMPOUND LIGHT MICROSCOPE

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6
Q

unstained organisms are observed against dark background

A

DARKFIELD MICROSCOPE

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6
Q

used to observe morphology of microorganisms

A

BRIGHTFIELD MICROSCOPE

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7
Q

can be used to observe unstained living
microorganisms

A

PHASE-CONTRAST MICROSCOPE

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8
Q
  • fluorescent dye attached to organisms
    – primarily an immunodiagnostic technique
    – used to detect microbes in cells, tissues and clinical specimens
    – contain a built-in ultraviolet light source when it strikes certain dye and pigment, it
    glows against dark background
A

FLOURESCENCE MICROSCOPE

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9
Q

Two types of Electron Microscope:

A

TRANSMISSION ELECTRON MICROSCOPE(TEMs)
SCANNING ELECTRON MICROGRAPHS(SEMs)

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9
Q

uses an electron beam as source of illumination and magnets to focus the beam

A

ELECTRON MICROSCOPE

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10
Q

magnify further the image formed

A

OCULAR LENS/EYEPIECE

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10
Q

Components of the Compound Light Microscope

A

OCULAR LENS/EYEPIECE
Revolving nosepiece
OBJECTIVE LENSES
STAGE
IRIS DIAPHRAGM CONTROL ARM
CONDENSER
COLLECTOR LENS WITH FIELD
DIAPHRAGM
RHEOSTAT CONTROL KNOB
FIELD DIAPHRAGM LEVER
ON/OFF SWITCH
BASE
CONDENSER CONTROL KNOB
FINE and COARSE ADJUSTMENT KNOB
BINOCULAR BOD
ARM

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10
Q

enables scientists to observe living cells at extremely high magnification

A

ATOMIC FORCE MICROSCOPE

10
Q

– specimen is viewed on screen
– has a tall column at the top of which an electron gun fires a beam of electrons downward
– has excellent resolution that allows examination of cellular and viral ultrastructure
– specimen is not living and reveals internal features of thin specimens

A

TRANSMISSION ELECTRON MICROSCOPE(TEMs)

10
Q

holds objectivce lenses

A

Revolving nosepiece

11
Q

– specimen is viewed on screen
– has a shorter column and instead of being placed into the electron beam, the
specimen is placed at the bottom of the column
– gives illusion of depth (2D) which is useful for examining surface features of cells and virus
– specimen is nonliving and resolution is less that of TEM

A

SCANNING ELECTRON MICROGRAPHS(SEMs)

12
Q

used to magnify objects placed on the stag

A

OBJECTIVE LENSES

13
Q

flat surface on which the specimen is placed

A

STAGE

14
Q

used to adjust the amount of light passing through the condenser

A

IRIS DIAPHRAGM CONTROL ARM

15
Q

contains a lens system that focuses light onto the specimen

A

CONDENSER

16
Q

controls the amount of light entering the condenser

A

COLLECTOR LENS WITH FIELD DIAPHRAGM

17
Q

used to adjust the amount of light passing through the collector lens

A

FIELD DIAPHRAGM LEVER

17
Q

controls the amount of light emitted from the light source

A

RHEOSTAT CONTROL KNOB

18
Q

turns the light source on and off

A

ON/OFF SWITCH

19
Q

contains the light source

A

BASE

20
Q

used
to adjust the height of the condenser

A

CONDENSER CONTROL KNOB

21
Q

used to focus the objective lenses

A

FINE and COARSE ADJUSTMENT KNOB

22
Q

holds the ocular lenses in their proper locations

A

BINOCULAR BODY

22
Q

supports the binocular body and the revolving nosepiece

A

ARM

23
Q

low power objective

A

X 10

24
Q

scanning objective

A

X4

25
Q

high dry objective

A

X 40

26
Q

oil immersion objective

A

X 100

microbiology (17 decks)

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