BACTERIA II Flashcards
Living together of two different organisms or population
SYMBIOSIS
types of symbiosis:
parasitism
mutualism
commensalism
one organism lives in or exploits another
PARASITISM
both organisms or populations are benefits
MUTUALISM
symbiosis is Identified/Classified by:
cell morphology
staining procedures
fixation
two organisms live in association and one is benefited while the other is neither benefited nor harmed
COMMENSALISM
Characteristics of symbiosis
Bergey’s Manual of Systematic Bacteriology:
23 phyla 32 classes 5 subclasses 77 orders 14 suborders 182 families 871 genera 5,007 species
symbiosis is categorized by:
gram-positive with cell wall
gram-negative with cell wall
no cell wall
types of cell morphology
cocci (diplococci, streptococci, staphylococci, tetrads, octads)
pleomorphic (variety of shapes)
bacilli (diplobacilli, streptobacilli, coccobacilli, long filaments, branched)
spirilla (single or in pairs, spirochetes)
staining procedures category and examples
simple straining procedure (staining in methylene blue)
structural straining procedure (capsule, flagella, endospore stain)
differential straining procedure (gram, acid-fast stain)
Fixation types:
kills the organism
preserves morphology
anchor smear to slide
SIMPLE STAINING steps:
smear loopful of microbes onto slide
air-dry
drip methanol onto specimen to fix
flood slide with stain
rinse with water and blot dry
examine with x100 objectives
most spiral-shaped and about half of bacilli use flagella for movement
Motility
is based on the size, color, shape, elevation, and margin appearance of colonies that are grown in pure culture on an agar plate
Colony morphology
Atmospheric requirements
obligate aerobes
microaerophilic aerobes
facultative anaerobes
aerotolerant anaerobes
obligate anaerobes
capnophiles