Microbial life at high temperatures Flashcards
high t, change in protein sequence?
so the enzymes are not denatured by the high
temperature and can stay active.(enzymes are heat-stable)
thermophilics?
Transport across the membrane functions optimally at high temperature.
their cytoplasmic membrane requires?
Requires modification of the cytoplasmic membrane so it remains stable at
high temperatures
for example,cytoplasmic modification?
lipid monolayer of Archaea
need proteins that are
Heat‐shock proteins which help keep proteins in the active conformation.
and protection mechanism
to ensure stability of DNA (GC rich).
plus de CG?
pcq plus de hydrogen bond resistant
Heat‐stable enzymes can be useful for
biotechnology (taq polymerase)
Endospores help resist high temperatures but
they are not metabolically active).
tag polymerase?
a technique for amplifying DNA and a major tool of modern biology