enzyme kinetics

Question 1

in the Vo formule when is Vo =V max?

Answer 1

when there is too much substrat, when u add something it will be negligeable

Question 2

What is Km?

Answer 2

substrate concentration when half the activate site are saturated at half maximum velocity

Question 3

Km low means?

Answer 3

enzyme reaches quicker Vmax and that enzyme binds more tightly

Question 4

high Km means?

Answer 4

weak binding

Question 5

how do we obtains the graphic with a line

Answer 5

put the equation under one

Question 6

what are the Units of Km?

Answer 6

uM

Question 7

E+S- ES–P , km represente kel etape?

Answer 7

E+S–ES

Question 8

E+S- ES–P + E ,Vmax represente kel etape?

Answer 8

ES-P­­­

Question 9

k2 equals to ?

Answer 9

Kcat

Question 10

which is?

Answer 10

Vmax/(E)t

Question 11

what does (E)t represent?

Answer 11

total enzyme concentration

Question 12

What is Kcat?

Answer 12

turnover number of enzyme(how fast it liberates P and how are fast can be utilizable)

Question 13

V max what is it?

Answer 13

when enzyme are fully saturated with substrate

Question 14

turnover number is?

Answer 14

Number of S molecules convreted to P per enzyme per unit time

Question 15

how can i define which enzyme is doing a better job?

Answer 15

K cat/km

Question 16

why we K cat / Km ?

Answer 16

because we can’t decide only looking one we need a ratio

Question 17

the bigger ratio means

Answer 17

the higher efficiency

Question 18

Enzyme inhibition can be classed into four types on

Answer 18

• Competitive
• uncompetitive
• noncompetitive
• irreversible Inhibition

Question 19

Irreversible inhibitors (inactivators) react with

Answer 19

an enzyme

Question 20

what is his job of the irrerversible inhibitors?

Answer 20

One inhibitor molecule can permanently shut off one enzyme molecule

Question 21

therefore, inhibitor bind with the enzyme?

Answer 21

covalently

Question 22

but competitive,uncompetitive and non competitive bind?

Answer 22

do not form covalently bonds with the enzyme

Question 23

the graphic of MM is?

Answer 23

vo and S

Question 24

why is the graphic vo and m,what about enzyme ?

Answer 24

because the enzyme concentration is the same

Question 25

what is the purpose of that graphic MM?

Answer 25

how the velocity changes with amount of substrate

Question 26

if number of enzyme decreases,Vmax%

Answer 26

decreases

Question 27

Kcat represents?

Answer 27

( umol substrate converted )/( umol enzyme active site : min-1)

Question 28

Vmax what are the units?

Answer 28

s-1

Question 29

what are all the units of the formula?

Answer 29

(umol)/(umol` min-1)