enzyme kinetics Flashcards
in the Vo formule when is Vo =V max?
when there is too much substrat, when u add something it will be negligeable
What is Km?
substrate concentration when half the activate site are saturated at half maximum velocity
Km low means?
enzyme reaches quicker Vmax and that enzyme binds more tightly
high Km means?
weak binding
how do we obtains the graphic with a line
put the equation under one
what are the Units of Km?
uM
E+S- ES–P , km represente kel etape?
E+S–ES
E+S- ES–P + E ,Vmax represente kel etape?
ES-P
k2 equals to ?
Kcat
which is?
Vmax/(E)t
what does (E)t represent?
total enzyme concentration
What is Kcat?
turnover number of enzyme(how fast it liberates P and how are fast can be utilizable)
V max what is it?
when enzyme are fully saturated with substrate
turnover number is?
Number of S molecules convreted to P per enzyme per unit time
how can i define which enzyme is doing a better job?
K cat/km
why we K cat / Km ?
because we can’t decide only looking one we need a ratio
the bigger ratio means
the higher efficiency
Enzyme inhibition can be classed into four types on
- Competitive
- uncompetitive
- noncompetitive
- irreversible Inhibition
Irreversible inhibitors (inactivators) react with
an enzyme
what is his job of the irrerversible inhibitors?
One inhibitor molecule can permanently shut off one enzyme molecule
therefore, inhibitor bind with the enzyme?
covalently
but competitive,uncompetitive and non competitive bind?
do not form covalently bonds with the enzyme
the graphic of MM is?
vo and S
why is the graphic vo and m,what about enzyme ?
because the enzyme concentration is the same
