lab 2

Question 1

two most commonly used culture media?

Answer 1

Nutrient Broth(NB) and Nutrient Agar (NA)

Question 2

Agar melts at?

Answer 2

90 - 100

Question 3

Agar cools at?

Answer 3

40-45

Question 4

how to prepare a agar plates?

Answer 4

1- NA bottle,wipe water from the outside of the bottle
2- Flame the lip of the bottle
3- Pour in the molten agar
4- Rinse the empty bottle with hot water

Question 5

Agar plates are always incubated in an ?

Answer 5

inverted position

Question 6

why?

Answer 6

to prevent condensation that may form on the lid and falling on the culture

Question 7

What technique are we using from putting colonies in agar in dilution?

Answer 7

pour plate technique

Question 8

before dilution what technique were we using?

Answer 8

Spread plate technique

Question 9

do we have to change the tip when we put dilution in agar?

Answer 9

no more

Question 10

whats the formula for calculating the number of colonies forming units per ml of original culture ?

Answer 10

CFU= (number)-(dilution x volume)