Continuous culture Flashcards
continous culture is more a mimic?
closely mimic of the environment
why open systems?
- Cst supply of nutrients and diffusion of waste
- competition with other microorganisms.
- Predation
- changing environmental conditions.
Over time, most environmental systems
can reach?
an equilibrium
An equilibrium?
division rate
equals death rate
What can be used in the lab to keep the microorganisms in a cst growth?
chemostats
what’s chemostats?
type of continuous culture
In the chemostat, two factors govern the specific growth rate and cell density,?
- Fresh medium: supply of limiting nutrients
- Overflow: death of microorganism
What goes inside in the chemostat?
Fresh medium: Supply of limiting nutrients
what are the chemostat parameters?
- Concentration of a limiting nutrient
- Dilution rate
Concentration of a limiting nutrient comes from?
Fresh medium and Aeration
THe dilution rate is?
Addition of fresh medium and washout
When dilution rate is low
doesn’t work really well
Once equilibrium is reached ?
nbr of cells is cst, growth rate = the death rate
