Microbial Communities Flashcards
Examines interactions between species in groups over
a wide range of temporal and spatial scales, including
distribution, population dynamics, structure,
abundance, and demography
community ecology
community ecology is also called
synecology
important aspects of microbial communities
community boundary
community structure
community strucrture can be described by
❑Species makeup
❑Species richness
❑Species relative abundance
an assemblage of microbial cells that is irreversibly
associated (not removed by gentle rinsing) with a surface and
enclosed in a matrix of primarily polysaccharide material
biofilm
Structured communities of
one or more species of
microorganisms.
biofilm
biofilm produces a community of members of a matrix characterized by a ___
hydrogel
a key stage in the biofilm lifecycle, involves the transition from initial microcolonies to a complex, three-dimensional structure, characterized by increased cell density and the production of an extracellular matrix (EPS
biofilm maturation
adhesive structures in biofilm formation
PIA
eDNA
proteins
disruptive factors in biofilm formation
proteases
nucleases
PSMs
- considered a specialized type of
biofilm.
biomats
range from several millimeters to a
centimeter thick, and are vertically
stratified into distinct layers versus
biofilms which are typically one to
several cell layers thick
biomats
microbial
groups are tightly compressed into a
thin mat of biological activity)
biomats
commonly associated with aquatic
habitats, including hot springs,
hypersaline ponds, and intertidal
coastal zones and oligotrophic
environments
biomats
first layer of biomat
microalgae
second layer of biomat
cyanobacteria and diatom species
(oxygenic phototrophs, aerobic heterotrophs
3rd layer in biomat
purple sulfur and green sulfur bacteria (anoxygenic phototrophs
anaerobic heterotrophs)
fourth layer in biomat
sulfate reducers
methanogens
iron sulfide rich sediments
Sedimentary layer, CH4 and H₂S formation
which layer
sulfate layers, methanogens, iron sulfide rich sediments
- It is the regulation of gene expression in response to
fluctuations in cell-population density.
quorum sensing
- Quorum sensing bacteria produce and release chemical signal
molecules called
autoinducers
that increase in concentration
as a function of cell density
autoinducers
gene expression produces
rna and protein
study quorum sensing and explain it
Lux Quorum Sensing in Vibrio fischeri
Autoinducer Production:
The process begins with the production of an autoinducer molecule, specifically N-acyl homoserine lactone (AHL), synthesized by the enzyme LuxI.
As the bacterial population grows, the concentration of AHL increases in the environment.
Detection and Activation:
When the concentration of AHL reaches a threshold level, it binds to the LuxR protein, a transcriptional activator.
The LuxR-AHL complex then binds to the promoter region of the lux operon, activating the transcription of genes responsible for bioluminescence.
Bioluminescence:
The lux operon includes genes such as luxA and luxB, which encode the α and β subunits of luciferase, the enzyme that catalyzes the light-emitting reaction.
Additional genes, luxC, luxD, and luxE, are involved in synthesizing the fatty aldehyde substrate required for the bioluminescent reaction.
Regulation and Efficiency:
The system ensures that bioluminescence occurs only at high cell densities, making it an energy-efficient process.
This mechanism allows Vibrio fischeri to produce light in a synchronized manner, which is beneficial for symbiotic relationships, such as with the Hawaiian bobtail squid