Introduction to Microbial Ecology Flashcards
study of microbes
within habitats, and their beneficial and detrimental
impacts on human health and welfare.
environmental microbiology
– focuses on the interactions of
microorganisms within environment such as air,
water and soil.
microbial ecology
microbial interactions factors
biological
chemical
physical
biological factors can either be
competition
predation
symbiosis
symbiosis can be
mutualism
commensalism
parasitism
focuses on interaction of microorganisms with each other
(ex. bacteriophage)
microbial ecology
is an interaction between organisms or species in which both require a resource that is in limited supply (such as food, water, or territory)
competition
is an interaction between organisms or species in which both require a resource that is in limited supply (such as food, water, or territory)
predation
is a long-term biological interaction between two or more organisms of different species
symbiosis
Both organisms benefit from the relationship. For example, bacteria and their hosts, where the bacteria synthesize vitamin K for the host
mutualism
One organism benefits, while the other is not affected.
commensalism
One organism benefits, while the other is harmed
parasitism
chemical factors include
chemotaxis
is a chemical that draws cells to a specific area by stimulating them to move towards it
chemoattractant
is a substance that causes cells or organisms to move away from it
chemorepellent
physical factors include
sunlight
temperature
salinity
communication of
microorganisms based
on cell density
quorum sensing
can be seen in
the sliminess of the teeth
biofilm
example of microbial interactions
bacteria-fungi
bacteria-bacteria
fungi-fungi
applications of microbial interactions
meical
food
agriculture
cell modeling
focuses on an environment’s influence to the diversity, distribution, and abundance of microbes
microbial ecology
shape the structures and composition of microbial communities that inhabit natural and built environments
physical factors
chemical factors
biological constraints
- s the study of the structure and function of entire nucleotide sequences isolated and analyzed from all the organisms (typically microbes) in a bulk sample
metagenomics
equivalent of tissue, organ, or entire microorganism
colony
equivalent of a single microorganism
microcolony
is associated with microbial production of shortchain fatty acids (SCFA) and bacterial diversity
intense exercise
- ”It is increasingly recognized
that the gut microbiota
profoundly influences many
aspects of host development
and physiology, including the
modulation of brain
development and behavior.
microbial gut brain axis
The gut and brain communicate through the
gut-brain axis
why do we study microbial ecology
- Microbes cause diseases of macroscopic organisms,
including humans - Much of our food depends on microbes
- Microbes degrade and detoxify pollutants
- Microbes can be useful model systems for exploring
general principles in ecology and evolution - Some microbes are examples of early life on earth and
perhaps of life on other planets
Microbes mediate many biogeochemical processes that
affect global climate - Microbes are everywhere, doing nearly everything
is a chemical process that breaks down organic compounds without oxygen
fermentation
s a process that uses living organisms to clean up environmental pollutants.
bioremediation
also known as a transposable element or transposon, is a segment of DNA that can move around within a genome.
jumping genes
was a Nobel Prize-winning geneticist who discovered that genes can move, or “jump”, to new locations on chromosomes
Barbara McClintock
creates biofilm and a rock formation
cyanobacteria
cyanobacteria is also called as
blue green algae
was a period when oxygen levels in Earth’s atmosphere increased for the first time.
the great oxidation event
what eon has the primary rise of oxygen in the atmoshpere
phanerozoic (bit of proterozoic)
published drawings of bacteria showing rods, cocci, and spiral
Antonie van Leeuwenhoek
when was the formation of Earth
4.5 BYA
According to the most widely accepted theory, the early Earth that collided with Theia to form the moon is called “proto-Earth” - essentially the early version of Earth before the impact with Theia occurred; this collision is known as the
Giant Impact Hypothesis.
The “giant impact hypothesis” is a widely accepted scientific theory that proposes the ___ formed from debris ejected into space after a Mars-sized object collided with the early Earth,
moon
The object that collided with Earth is often called
Theia
Volcanic and hydrothermal activities released of various gases into the
atmosphere
dominant ones are: (4)
N2 (nitrogen)
, CO2 (carbon dioxide)
, CH4 (methane)
, NH3 (ammonia)
Earth’s precellular state can be characterized as highly ____à
reducing power responsible for the formation of the first organic
compounds
anaerobic
early life forms are utilizing(as
donor or souce)
chemolithotrophs, methanogens
etc.
hyperthermophilic
hyperthermophilic archaea using
S0 as the ___acceptor or
those use of Fe3+ , SO4
2−, NO3−,CO2, or O2
as ___acceptors.
electron
means using light to produce energy and create organic compounds
phototrophic
is an organism that obtains energy by the oxidation of electron donors in their environments.
chemotroph
is an organism that can produce its own food using light, water, carbon dioxide, or other chemicals
autotroph
an organism that eats other plants or animals for energy and nutrients.
heterotroph
are a diverse group of organisms using an inorganic substrate (usually of mineral origin) to obtain reducing equivalents for use in biosynthesis
lithotroph
is an organism that obtains hydrogen or electrons from organic substrates.
organotroph
study the major nutritional types of prokaryotes
CHO
PAL
example of PAL
cyanobacteria
example of CHO
most bacteria, some archaea
example of PHO
some purple and green bacteria
example of CAL
bacteria and many archaea
- ___photo-driven process by 3 bya.
anaerobic
- Followed by chlorophyll-containing
anoxygenic bacterial photosynthesis
involving ___ and ___bacteria where
H2S was the electron source
purple and green
- Followed by chlorophyll-containing
anoxygenic bacterial photosynthesis
involving purple and green bacteria where
___ was the electron sourc
hydrogen sulfide
- Microbes to ___ land by 2.75 bya
dry
___ with oxygenic
photosynthesis produced the aerobic
atmosphere
cyanobacteria
O2
level in Earth’s atmosphere increased
and by ∼1.78–1.68 bya oxygen
respiration could have been used to
support the growth of the first ___
single cell eukaryotes
___produced from O2
shielded the
earth.
ozone (O3)
are mats of
cyanobacteria- a
stromatolites
era from earliest to latest
Hadean
Archaean
Proterozoic
Cambrian to Present
- finely laminated sediments formed by the
accretion of both detrital and biochemical precipitates on
successive layers of microorganisms
stromatolites
abiotic to biotic transition has three phases
pre-darwinian
proto-darwinian
darwinian
includes prebiotic synthesis of organic compounds
pre-darwinian
membranous structures enveloping organic compounds
proto-darwinian
includes the first cell, evolution of cellular systems into bacteria and archaea > eukaryotes
darwinian
nvolve astrophysical and
geochemical activities
pre-darwinian
___enclosed with
membrane-like structures
were involved in the
formation of organic
molecules
vesicles
life arose from a “___ ___” in a lake on the
surface of Eart
primordial soup
organic compounds presumably
accumulated in the environment
what time
proto-darwinian
could have
included nucleic acid inside a vesicle
and that the vesicle had a mechanism
for generating an ionic charge across
the membrane barrier
prebiotic compounds
self-replicating single-stranded RNA
with auto- catalytic activity, ____
could provide a basis for development
of molecular biology in this
evolutionary process
ribozyme
replaced RNA as the molecule
carrying genetic information
DNA
what time did DNA replaced RNA as the molecule
carrying genetic information
proto-darwinian
- Cell had the capability for
heredity - Evolution leading to different
lifestyles and life forms, periodic
environmental changes
providing the selective pressure
that led to new cell types. - genetic variation in these
asexual microorganisms would
be attributed to mutations and
horizontal (lateral) gene transfer
which time
darwinian
establishment of a nucleus prior to the development of mitochondria and
chloroplasts by
endosymbiosis
mitochondria and chloroplast reproduce by ___
binary fission
- It is a theory that the first eukaryotic cells were created when two symbiotic
organisms fused their genomes.
gene fusion hypothesis
in gene fusion hypothesis, ___ ___arose spontaneousl
nuclear membrane
- One bacterial species has a “___” nuclear membrane
primitive
prokaryotic DNA is ___
simple and circular
eukaryotic DNA is ___ and has ___
linear
introns
exons
are noncoding sections of an RNA transcript, or the DNA encoding it, that are spliced out before the RNA molecule is translated into a protein.
introns
regions of DNA that are transcribed into RNA and become part of a mature messenger RNA (mRNA) molecule
exons
addresses the origin of chloroplasts and mitochondria where both of these
organelles developed from bacteria.
endosymbiotic hypothesis
genome of __ ____, an Alphaproteobacteria, is remarkably similar to
the mitochondrial genom
Ricketssia prowazekii
genome of Rickettsia prowazekii, an ___, is remarkably similar to
the mitochondrial genom
alphaproteobacteria
- chloroplasts in green algae and higher plants …evolved from
Prochloron
because it is the only aerobic photosynthetic cell that has both
chlorophyll a and b.
prochloron
mitochondria is from your __
mother
t is a model that explains how
metabolic pathways evolved from
primitive enzymes
patchwork hypothesis
It suggests that these enzymes
were promiscuous, meaning they
could react with many chemically
related substrates.
patchwork hypothesis
s a biological process where genetic material is transferred between two organisms through direct contact. It’s a type of sexual reproduction that occurs between organisms that don’t normally reproduce sexually, such as bacteria, fungi, and protozoa.
conjugation
is a process that involves the combination of genetic material from two different individuals to create a new organism.
sexual reproduction
is a process that creates offspring that are genetically identical to their parent. It doesn’t involve the fusion of gametes, or reproductive cells.
asexual reproduction
These enzymes were slow and nonspecific, which allowed primitive
cells with small genomes to
overcome their limited coding
capacities
primitive enzymes
selected phenotypic characteristics of B,A,E
dissimilatory reduction of SO4 or Fe
B - Yes
A- Yes
E - No
selected phenotypic characteristics of B,A,E
Nitrification
B - Yes
A- Yes
E - No
selected phenotypic characteristics of B,A,E
Denitrification
B - Yes
A- Yes
E - No
selected phenotypic characteristics of B,A,E
Nitrogen Fixation
B- Yes
A- Yes
E - no
selected phenotypic characteristics of B,A,E
chemolithotrophy
B - Yes
A - Yes
E- No
selected phenotypic characteristics of B,A,E
methanogenesis
B - no
A - yes
E - no
selected phenotypic characteristics of B,A,E
oxygenic photosynthesis (chlorophyll based)
b - yes
a- no
e - yes
selected phenotypic characteristics of B,A,E
anaerobic photosynthesis (chlorophyll based)
b - yes
a - no
e - no
selected phenotypic characteristics of B,A,E
rhodophsin-based energy metabolism
b - yes
a - yes
e - no
is a visual pigment found in rod photoreceptor cells in the vertebrate retina
rhodopsin
use
chemical energy from
organic compounds,
minerals, and light-driven
reactions.
microbial cells
growth curve where there are depleted resources
death phase
growth curve where there are carrying capacity
stationary phase
growth curve where there are rapid decline of nutrients
log phase
growth curve where they show that there are resource competition already
log phase
Closely related organisms that can
interbreed
eukaryotic species
___ currently considered most important for grouping strains
together as a species
traits
or greater genomic DNA–DNA hybridization
70%
or greater identity (3% difference) in 16S rRNA gene
sequence.
97%
-based systematics, in which ecologically distinct species
are recognized
ecotype
analysis of gene content of an organism by sequencing and mapping of genomes (chromosomes of eukaryotes or nucleoid of prokaryotes)
genomics
analysis of gene content of all organisms in a specific environment
metagenomics
study evaluating the production of mRNA produced at a specific time by a cultured organism
transcriptomics
study of protein structure and protein regulation of an organism
proteomics
analysis of all proteins produced by all the organisms in a specific environment
metaproteomics
`study of small molecules and intermediate compounds produced from metabolism frequently includes the end products of metabolism
metabiomics
study of the various metal ions and their activities in a biological cell
metallomics
study of all the biological systems and biochemical components of cellular system
biolomics
study including all the microorganisms and their interactions with the immediate environment
microbiomics
can provide clues about the physiology and thus potential
biogeochemical role of uncultivated microbes.
metagenomics