micro lab quiz #3 Flashcards
what do you use or antimicrobial susceptibility testing
disk diffusion method
when did bacteria start to become resistant to antibiotics
1950
who started using disk diffusion method
Kirby and bauer in 1966
how long are the filet paper with antibiotics
6mm
what are the 6mm paper discs impreganated with
standard concentration of antibiotic
what agar is used for standard specifications in diffusion disc method
Mueller-Hinton agar plate
what is used to have a standard suspension of organism
McFlarland standard
how does disc diffusion work
the rate of diffusion through agar is dependent on diffusion of solubility of the drug on the mueller-winton agar
what is diffusion heavily ties to
molecular weight
antibiotic
chemicals that have select toxicity
broad spectrum
works against GNR AND GPR
narrow spectrum
work against GNR or GPR not both
narrow spectrum is used if possible
doesn’t get as much microbiome
disc diffusion rules
the antibiotic can only kill if it touches
tiny mcg
can reach super far in 12 hours
large mcg
can’t reach as far as tiny mcg
difference between tiny mcg and large mcg is
spreading rate of microorganism
bacteriostatic
stop production and allows your immune system to catch up
bactericidal
antibiotic kills bacteria * more side effects
zone of inhibition are measured bu
mm
is endospores kills in pasteurization
no
pasteurization
super high heat then rapid cooling
what does pasteurization kill
most pathogens