lab quiz 1 micro Flashcards
what is a common thing to use in an agar
tsa
if you want culture what media would you use
nutrient broth
what media would you use for culture and isolation
agar
first clinical lab coat
1968
1 colony is
from one bacterium, all same type, 1000’s of bacteria
why do you need immersion oil
increase resolving power of the microscope
does bacteria have corners
no
why do you do broth or agar
to see if there can be growth of bacteria
examples of stats
blood, csf, aspirates, tissue, deep wound
ex of non stats
throat urine nose feces
which media is faster
broth
what is the technique called for isolation in petri plates
streak technique
Why are petri plates incubated upside down?
so that condensation does not drip onto the plate and interfere with the developing microbes
with an agar what goes in
oxygen
with an agar what goes out
gaseous waste
what can be included in the agar
nutritive media, water, proteins, carbs, gel protein
red lense in microscope
4x
yellow lense
10x
blue lense
40x
white lease
100x
need for immersion oil?
oil immersion lease in tiny tin diameter, so needs more light
how should the microscope be when put away
small objective lease set to smallest and stage cranked way down
which characteristic would you use a microscope to see it with
margins
cultural characteristics of agar
forms (shape), size, color, elevation(high), margins
types of forms characteristics
circular, irregular, rhizoid
types of elevation characteristics
flat, raised, convex, umbonate
types of margins characteristics
entire, lobate, undulate, filamentous, serrate
what is the tool called when doing the streak technique
inoculating loop
part of broth on top
pellicle
part of broth that sinks to bottom
sediment
