Metabolism Flashcards
What is glycolysis?
turning glucose into pyruvate
What determines if something is reversible?
the delta G
What are two big purposes of Acetyl CoA?
- enter TCA
2. if ATP is high, it can make fatty acids
What transporter does the brain use?
GLUT3
What transporter does muscle use?
GLUT4
What transporter does adipose tissue use?
GLUT4
What transporter does the liver use?
GLUT2
Of the 4 big glucose using organs/tissues, which ones have transporters that are insulin dependent?
Muscle and Adipose have insulin dependent transporters.
Where does the TCA take place?
Mito
How big are the porins in the mito’s external outter membrane? How big is ATP, NADH and AAs?
5kD.
ATP = 507
NADH = 663
AAs = ~100
Where does OxPhos occur?
The inner-membrane?
Where do the protons end up after going through the ATPase?
Matrix.
If you block hexokinase what can happen to glucose?
It can go in and out of the cell. G6P cannot leave the cell.
What is Km?
the substrate concentration at which an enzyme is at half its Vmax.
What does PFK-2 do?
It increases glycolysis by helping to making F 2,6-P.
Does the muscle make glycogen?
yes. But it can’t release any glucose into the blood.
What is the Henderson-Hasselbach equation?
pH = pKa + log [A-]/[HA]
What is the Michaelis-Menton equation?
Vmax[S]/(Km + [S])
How does a line Weaver-Burke plot line up?
X-axis = -1/Km Y-axis = 1/Vmax
Does F-2,6P increase or decrease in response to insulin?
Increase
What does epinephrine do to the muscles and the liver?
Muscle: increase muscle F26P2 and increases glycolysis.
Liver: decreases F26P2, and decreases glycolysis
Does a higher Km mean more or less affinity?
less.
Which tissues have GLUT transporters with a higher Km?
Liver and gut.
