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Media Flashcards

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Biology 101 flashcards
1
Q

What is the purpose of MAC?

A

Selective and differential for G-

Pink - LF
Yellow/clear - NLF

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2
Q

What is the function of EMB?

A

Differentiate LF and NLF
LF - purple / green metallic sheen
NLF - colorless

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3
Q

What is the function of Hektoen Enteric?

A 
Selects for G- enterics 
Differentiate salmonella and shigella
Salmonella - H2S black center
Shigella- clear/green (pathogen) 
Yellow - nonpathogen
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4
Q

What is the function of XLD agar?

A

Select for G- enterics
Differentiate salmonella and shigella

Salmonella- red with H2S black center
Shigella - clear pink to red

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5
Q

what is the selective agent in MSA?

A

7.5 - 10% salt, carbohydrate-alcohol mannitol, phenol red

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6
Q

what does MSA select for?

A

staph species

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7
Q

what does it mean when the MSA turns yellow?

A

the organism ferments mannitol and created an acidic end product

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8
Q

what is the selective agen in CNA?

A

antibiotics: colistin and nalidixic acid

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9
Q

what does CNA inhibit?

A

G- bacteria

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10
Q

what is the purpose of TCBS agar?

A

to select for Vibrio spp and differentiate V cholerae and other spp

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11
Q

what does a positive TCBS look like?

A

yellow colonies (V. cholerae)

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12
Q

what are the inhibitors in MAC?

A

bile salts and crystal violet

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13
Q

what are the selective agents in Modified Thayer Martin?

A

vancomycin
colistin- G-
nystatin
trimethoprim

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14
Q

what is New york City media made out of?

A

lysed horse blood
horse plasma
yeast dialysate
antibiotics

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15
Q

what media is used for the isolation of Bordetella pertussis?

A

Bordet-gengou

Regan Lowe

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16
Q

what is CIN agar used for?

A

Yersinia

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17
Q

what is Martin Lewis media used for?

A

isolation of N. gonorrhoeae

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18
Q

what media is used for Mycobacterium?

A

middlebrook

petraganis

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19
Q

what supplement is added to BBA?

A

vitamin K1

hemin

20
Q

what is BLKV agar used for?

A

isolate GNR obligate anaerobes, esp bacteroides, prevotella, fusobacterium, veillonella

21
Q

what is CBA used for?

A

aerotolerance testing

22
Q

what is BBE selective for?

A

B fragilis

23
Q

what is PEA agar used for?

A

isolation of G+ and G- obligate anaerobes and G+ facultative anaerobes

24
Q

what is egg yolk agar used for?

A

detection of lectithinase lipase and proteolysis for clostridium

25
Q

what is cooked meat agar used for?

A

clostridium

26
Q

what is Todd Hewitt broth used for?

A

grow strep for serotyping

27
Q

what agars are used for Mycobacterium spp?

A

Lowenstein Jensen
Middlebrook 7H10
Petragnani

28
Q

what is anaerobic blood agar enriched with?

A

hemin
cystine
vitamin K
yeast

29
Q

what is BCYE agar used for?

A

Legionella

30
Q

what is Fletchers medium used for?

A

Leptospira

31
Q

what agars are used for Corynebacterium?

A

Tinsdale

Loefflers

32
Q

bile esculin agar purpose

A

selective for enterococcus spp

33
Q

CCFA agar purpose

A

selective for C. difficile (yellow colonies)

34
Q

organism requires X and V factors

A

haemophilus

35
Q

What does a yellow colony on TCBS mean?

A

Ferments sucrose

36
Q

Yellow colonies on CCFA mean what

A

Fermentation of fructose

37
Q

Media used for Neisseria gonorrhoeae

A

MTM
New York City
Martin Lewis

38
Q

DCA purpose

A

Inhibits gram positive organisms and most enterics

isolation of salmonella and shigella

39
Q

DCA inhibitory factors

A

Citrate

Deoxycholate salts

40
Q

DCA Differentiates what?

A

LF- pink

NLF - colorless

41
Q

Cetrimide selective for

A

P aeruginosa

42
Q

What do yellow colonies on HE agar mean?

A

Sucrose fermentation

43
Q

CTA media purpose

A

Growth of Neisseria spp

44
Q

What affects the redox potential of anaerobic media

A

pH

45
Q

Broth used for anaerobic media to detect volatile fatty acids

A

PYG

Microbiology (17 decks)

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