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Microbiology > Gram Negative Rods > Flashcards

Gram Negative Rods Flashcards

1
Q 
Oxidase -
Oxidizer
Nonmotile
Will grow on MAC
Nitrate -
Coccobacilli
Penicillin R
Cat +
A

Acinetobacter baumanii

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2
Q 
Oxidase - 
Oxidize glucose,maltose 
MAC +
Lysine +
DNAse +
ONPG+
Motile polar tuft flagella 
Ammonia odor
A

Stenotrophomonas maltophilia

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3
Q 
Oxidase - 
Nonutilizer
MAC 
Nonmotile
Nitrate -
Coccobacilli
A

Acinetobacter lwoffi

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4
Q 
Oxidase +
Nonutilizer 
Nitrate to gas +
Fruity odor
Motile peritrichous flagella 
Urease -
A

Alcaligenes faecalis

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5
Q 
Oxidase +
Oxidize g, m, l, man
Gelatinase -
Urease +
Nitrate + 
Earthy odor 
Pink on Ashdown
A

Burkholderia pseudomallei

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6
Q 
Oxidase +
Glucose / maltose Oxidizer 
Gelatinase -
Urease +
Nitrate -
Lysine +
Yellow green pigment non fluorescen
motile
DNase -
A

Burkholderia cepacia

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7
Q 
Oxidase +
Oxidizer
Gelatinase +
Yellow fluorescence
Indole +
A

Flavobacterium meningosepticum

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8
Q 
Oxidase +
Oxidize g, f, x
Gelatinase +
Blue green fluorescence
Nitrate +
Metallic sheen 
Fruity/tortilla odor
Motile polar monotrichous flagella
42
A

Pseudomonas aeruginosa

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9
Q 
GF
OXIDASE +
Spot indole +
LF
CIN - bulls eye pink center
Does not require Na
DNase +
VP +
motile
A

Aeromonas

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10
Q

what is P. aeruginosa susceptible to?

A

Polymixin B

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11
Q

what organism is polymixin B resistant?

A

B. cepacia

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12
Q

Lactose -
nitrate +
yellow TCBS

A

V. cholera

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13
Q

lactose -
nitrate +
green TCBS

A

V. parahemolyticus

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14
Q 
microaerophilic
catalase +
Oxidase +
hippurate +
Urease -
Seagull appearance
Nalidixic acid S
Cephalothin R
Nitrate +
A

C. jejuni

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15
Q

what temperature does campy grow best?

A

42

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16
Q

Pseudomonas infections

A

Nosocomial- burn, wound, RTI, UTI, bacteremia
Swimmers ear
Keratitis

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17
Q

Stenotropomonas colonized what patients

A

Immunocompromised and cystic fibrosis

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18
Q

C jejuni environmental factors

A

CAMPY BAP
42
Increased CO2
5% O2, 10% CO2, 85% N2

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19
Q

Most common cause of bacterial diarrhea

A

C. Jejuni

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20
Q

C jejuni sources of infection

A

Chickens
Raw milk
Pets

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21
Q

Helicobacter pylori infections

A

Gastritis

Duodenal and peptic ulcers

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22
Q 
Rarely cultured
Rapid urease on gastric secretions 
Urea breath test 
Grow on no selective media at 37 
Capnophilic
A

Helicobacter pylori

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23
Q 
SBA, MAC, CHOC
NLF
large yellow colonies on TCBS
Oxidase +
string test +
A

Vibrio cholerae

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24
Q

V. Cholerae infections

25
Q

V. Cholerae transmission

A

Contaminated water

Seafood

26
Q

Halophilic
Green TCBS
Some lactose +
Oxidase +

A

Vibrio vulnificus

27
Q

V vulnificus infections immunocompromised

A

Septicemia

Water associated wound infections

28
Q

V vulnificus infections healthy people

A

Gastroenteritis

29
Q

Require 1% NaCl
SBA, MAC
blue green colonies TCBS
OXIDASE +

A

Vibrio parahemolyticus

30
Q

V parahemolyticus infection

A

Gastroenteritis

Water associated wound infections

31
Q

Aeromonas infections

A

Gastroenteritis
Wound infections
Septicemia
Meningitis

32
Q 
SBA, CHOC MAC
no growth TCBS 
oxidase +
Do not ferment mannitol
DNase -
VP -
NLF
A

Plesiomonas shigelloides

33
Q

Plesiomonas infections

A

Gastroenteritis

34
Q

What do all vibrio have in common?

A

Oxidase +
Require Na
Mannitol fermentation
Growth on TCBS

35
Q 
Mannitol -
Nitrate -
42 - 
Oxidase +
Motile 
Pyoverdin production
A

Pseudomonas putida

36
Q

What organism is usually associated with a lung infection related to cystic fibrosis?

A

P aeruginosa

37
Q

Can be associated with melioidosis

A

B pseudomallei

38
Q

Yellow pigment
Oxidase +
Nonmotile
No growth on MAC

A

Chryseobacterium

39
Q

Only campylobacter spp that hydrolyze hippurate

40
Q 
Nitrate -
Hippurate -
Urease +
Cephalothin S
Nalidixic acid R
A

Helicobacter pylori

41
Q

Darting motility organism

42
Q

Nonfermenter infections and population

A

opportunistic

infect debilitated and immunosuppressed hosts

43
Q

why is TSI not recommended for nonfermenters?

A

slow growth

produce minimal amounts of acidic end products

44
Q

P. aeruginosa pigments

A

pyocyanin

pyoverdin

45
Q

what test differentiates stenotrophomonas from pseudomonas and burkholderia?

A

oxidase (-)

46
Q

what organisms oxidize lactose?

A

burkholderia

stenotrophomonas

47
Q

what is the only DNAse + organism?

A

stenotrophomonas

48
Q

oxidase negative organisms

A

stenotrophomonas

acinetobacter

49
Q

key reactions for chryseobacterium genus

A

Penicillin R

Polymyxin B R

50
Q

nonmotile and oxidase positive

A

chryseobacterium
flavobacterium
moraxella

51
Q

nonmotile and oxidase negative

A

acinetobacter

52
Q

organism halophilic

A

vibrio except cholerae

53
Q

gold standard to id H. pylori

A

histological staining and culture of biopsies

54
Q

differentiate pseudomonas and aeromonas

A

Aeromonas- GF, indole +

Pseudomonas- oxidize glucose, indole -

55
Q

differentiate aeromonas and plesiomonas

A

Aeromonas- DNase +, ornithine -

Plesiomonas- DNase -, ornithine +

56
Q

0129 disk use

A

differentiate vibrio species from aeromonas and plesiomonas

57
Q

0129 organism resistant

58
Q

Differentiate Helicobacter and campylobacter

A

Helicobacter- urease +, nitrate-, hipp -

Campy- urease -, nitrate +, hipp +

Microbiology (17 decks)

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