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Enterobacteriaceae Flashcards

1
Q

What biochemical test are all enterobacteriacea negative for?

A

Oxidase

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2
Q

What are the lactose fermenters?

A 
Klebsiella
E. coli
Enterobacter
Serratia
Citrobacter
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3
Q

What are the non lactose fermenters?

A 
Proteus 
Edwardsiella
Salmonella
Providencia
Morganella
Yersinia
Shigella
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4
Q

LF
IMViC + -++
Urease +
Indole +

A

Klebsiella oxytoca

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5
Q 
LF 
IMViC + + - -
Urease -
H2S -
Lysine +
Ornithine +
Lactose +

Beta hemolytic
Large colonies

A

E. coli

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6
Q 
LF 
IMViC - - + + 
Ornithine +
Lysine +
Arginine -
A

Enterobacter aerogenes

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7
Q 
LF 
IMViC - - + +
Ornithine +
Lysine -
Arginine +
A

Enterobacter cloacae

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8
Q

NLF
IMViC - - + +
Ornithine +
Lysine +

A

Serratia

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9
Q 
LF 
IMViC - + - +
Ornithine -
Lysine -
H2S +
Urease +
ONPG +
A

Citrobacter freundii

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10
Q 
LF 
IMViC - + - +
Ornithine -
Lysine -
H2S -
A

Citrobacter koseri

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11
Q 
LF 
IMViC - - + +
Ornithine -
Lysine +
Urease +
Indole -
A

Klebsiella pneumoniae

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12
Q

NLF
IMViC + + - -
Urease +
H2S +

A

Proteus vulgaris

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13
Q

NLF
IMViC - + - +
Urease +
H2S +

A

Proteus mirabilis

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14
Q 
NLF
IMViC + + -
Urease -
H2S +
ONPG + 
Fish or reptiles!
A

Edwardsiella

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15
Q 
NLF
IMViC - + - V
Urease - 
H2S +
ONPG - 
Ornithine +
A

Salmonella non typhi

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16
Q 
NLF
IMViC -  + - V
Urease - 
H2S +
ONPG -
Ornithine - 
Lysine +
A

Salmonella typhi

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17
Q 
NLF
IMViC + + - -
H2S -
Ornithine +
PDA +
A

Morganella

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18
Q 
NLF 
IMViC + + - +
PDA +
Ornithine -
Urease +
A

Providencia stuartii

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19
Q 
NLF 
IMViC + + -
Sucrose fermenter
Urease -
Ornithine-
A

Yersinia pestis

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20
Q 
NLF 
IMViC + + -
Sucrose fermenter
Ornithine +
Urease +
Bulls eye on CIN
A

Yersinia entercolitica

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21
Q 
NLF 
IMViC - + - -
H2S -
Ornithine +
Nonmotile
A

Shigella sonnei

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22
Q

NLF
IMViC - + - -
H2S -
Ornithine -

A

Shigella dysenteriae

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23
Q

What organisms are H2S +?

A

Prince Edward Cooks Salmon

Proteus Edwardsiella Citrobacter Salmonella

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24
Q

What organisms are nonmotile at 37?

A

SKY
SHIGELLA
KLEBSIELLA
YERSINIA

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25
Q

What organisms are PDA positive?

A

PPM
PROTEUS
PROVIDENCIA
MORGANELLA

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26
Q

What organisms are positive for vogues?

A

SERRATIA
ENTEROBACTER
KLEBSIELLA

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27
Q

What color is E. coli O157:H7 on SMAC?

A

Colorless

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28
Q

what shigella spp is group A?

A

dysenteriae (most severe)

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29
Q

what shigella spp is group B?

A

flexneri

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30
Q

What shigella spp is group C?

31
Q

what shigella spp is group D?

A

sonnei (most common)

32
Q

what organisms are A/A on TSI?

A 
(sun over desert) (y/y)
S- serratia
E- escherichia
E- enterobacter
K- klebsiella
33
Q

what organisms are A/A H2S+ on TSI?

A

C- citrobacter
A- arizona
P- proteus

(cap the geyser)

34
Q

what organisms are K/A H2S + on TSI?

A 
(moon over desert)
C- citrobacter
A- arizona
S- salmonella
E- edwardsiella
35
Q

what organisms are K/A on TSI?

A 
moon over desert
S- shigella
Ci- citrobacter
P- providencia
P- plesiomonas
P- proteus
Y- Yersinia
36
Q

Test differentiates klebsiella and enterobacter and their reactions

A

Ornithine
Klebsiella -neg
Enterobacter- pos

37
Q

O antigen use

A

Serological grouping of salmonella shigella

38
Q

H antigen use

A

Serotype salmonella

39
Q

K antigen function

A

Envelope
Prevent phagocytosis
Increase virulence

40
Q

E. coli infections

A

UTI
Septicemia
Neonatal sepsis and meningitis
Diarrhea

41
Q

Edwardsiella infections

A

Opportunistic
Bacteremia
Wounds

42
Q

Bipolar staining organism

43
Q

Lactose negative

A 
Shigella
Edwardsiella
Salmonella
Citrobacter
Serratia
Proteus
Morganella
Providencia
Yersinia
44
Q

H2S +

A

Edwardsiella
Salmonella
Citrobacter
Proteus

45
Q

VP +

A

Klebsiella
Enterobacter
Serratia

46
Q

Urease +

A

Proteus

Morganella

47
Q

Citrobacter freundii infections

A

Nosocomial

48
Q

Klebsiella infections

A

Pneumonia
UTI
Septicemia

49
Q

Enterobacter infections

A

Opportunistic and nosocomial
UTI
RTI
wounds

50
Q

Serratia infections

A

Opportunistic
Pneumonia
Septicemia

51
Q

Proteus infections

A

UTI
Wound
Septicemia

52
Q

Providencia infections

A

UTI

Diarrhea

53
Q

Yersinia enterocolitica infections

54
Q

Enterohemorrhagic (EHEC /STEC) pathogenicity

A

Diarrhea
Hemorrhagic colitis
HUS
most common cause of renal failure in children

55
Q

EHEC disease mechanism and gram stain

A

Toxins

RBCs

56
Q

ETEC infections

A

Travelers diarrhea

57
Q

ETEC disease mechanism and gram stain

A

Toxins

No polys / RBCs

58
Q

EIEC infections

A

Bloody diarrhea

Dysentery like

59
Q

EIEC disease mechanism and gram stain

A

Invasive ness

POLYS / RBCs / mucus

60
Q

EPEC infections

A

Diarrhea in infants

61
Q

EPEC disease mechanism and gram stain

A

Adherence

No polys/ RBCs

62
Q

EAEC infections

63
Q

Differentiate VP + GNR

A

Klebsiella - ornithine -, oxytoca indole +
Serratia - NLF
Enterobacter - ornithine +, aerogenes arginine -, cloacae lysine -

64
Q

Differentiate H2S + GNR

A

Citrobacter - LF
Salmonella - indole -
Proteus - urease + , vulgaris- indole +
Edwardsiella - indole +

65
Q

Differentiate PD + GNR

A

Proteus vulgaris - ++- - H2S
Proteus mirabilis - -+-+
Morganella - ++- -
Providencia ++-+

66
Q

What organism is sometimes mistaken for salmonella and will agglutinate salmonella polyvalent antiserum?

A

Citrobacter freundii

67
Q

Drugs of choice for treatment of enterobacteriaceae infections

A

Aminoglycosides
Sulfamethoxazole-trimethoprim
3rd generation cephalosporins

68
Q

If K antigen is present what must be done to detect O antigen? Why?

A

Boiled for 30 minutes

Covers O antigen and is heat labile

69
Q

Kauffman white classification

A

Classify salmonella based on O and H antigens

70
Q

Only DNAse + organism

71
Q

Lysine +

A 
E. coli
Klebsiella
Enterobacter aerogenes
Salmonella
Serratia
72
Q

Indole +

A

Proteus vulgaris
Providencia
Morganella
E. coli

73
Q

ETEC E. coli produces a toxin similar to what other organism

A

Vibrio cholerae

74
Q

Antibiotics to treat infections

A

Aminoglycosides
Beta lactams
Quinolones

Microbiology (17 decks)

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