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Microbiology > FASTIDIOUS GNR > Flashcards

FASTIDIOUS GNR Flashcards

1
Q 
OXIDASE -
CATALASE +
UREASE -
INDOLE -
NITRATE +
BHI agar- STAR SHAPED AREAS ON COLONIES
A

Actinobacillus actinomycetemcomitans

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2
Q 
oxidase -
catalase +
G, MF
TM +
BCYE +
no growth on blood
Small yellow green growth
Bipolar staining
A

Francisella tularensis

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3
Q 
oxidase - 
catalase - 
growth on blood
Rats
fried egg appearance
A

streptobacillus

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4
Q 
oxidase -
catalase -
no growth on blood
urease +
capnophilic
A

Ureaplasma urealyticum

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5
Q 
oxidase -
catalase -
no growth BAP
urease -
arginine +
fried eggs appearance
A

Mycoplasma hominis

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6
Q 
oxidase -
catalase -
no growth on BAP
urease -
arginine -
A

Mycoplasma pneumoniae

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7
Q 
oxidase +
no growth on BAP
Bordet Gengou  (drops of mercury)
Regan Lowe (mother of pearl)
beta hemolytic
Urea +
A

Bordetella pertussis

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8
Q 
oxidase +
catalase +
gelatinase +
motile
no growth on BAP
L-cysteine
BCYE 
ground glass appearance
A

Legionella pneumophila

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9
Q 
OXIDASE +
no growth on BAP
chocolate agar growth
require X factor only (hemin)
catalase -
ALA-porph -
A

Haemophilus ducreyi

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10
Q 
OXIDASE +
no growth on BAP
chocolate agar growth
req V factor only (NAD)
ALA porph +
catalase -
A

Haemophilus paraphrophilus

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11
Q 
OXIDASE +
no growth on BAP
chocolate agar growth
Req V only
ALA proph +
catalase +
gamma hemolysis
A

Haemophilus parainfluenzae

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12
Q 
OXIDASE +
no growth on BAP
chocolate agar growth
Req V only
ALA proph +
catalase +
beta hemolysis rabbit/ horse blood
A

Haemophilus parahaemolyticus

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13
Q 
OXIDASE +
no growth on BAP
chocolate agar growth
Req  X & V 
ALA proph -
beta hemolysis
A

Haemophilus Haemolyticus

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14
Q 
OXIDASE +
no growth on BAP
chocolate agar growth 5-10% CO2
Req X & V 
ALA proph -
catalase +
gamma hemolysis
A

Haemophilus influenzae

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15
Q 
growth on BAP (48-72hr)
catalse -
indole -
Oxidase +
Nitrate +
A

Eikenella

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16
Q

what organism pits the agar, has a bleach odor, and is common in IV drug users?

A

Eikenella

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17
Q 
growth on BAP (48-72hr)
catalse -
lactose fermenter
ONPG +
ALA-porpho +
A

Haemophilus aphrophilus

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18
Q 
growth on BAP (48-72hr)
oxidase +
catalase -
NLF
ONPG -
slow indole +
nitrate -
capnophilic
A

Cardiobacterium hominis

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19
Q 
growth on BAP beta hemolytic (48-72hr)
GF
oxidase +
catalase -
NLF
indole -
H2S -
can grow on Neisseria media
A

Kingella

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20
Q

what are the members of the HACEK group?

A 
Haemophilus
Actinobacillus / aggregatibacter
Cardiobacterium
Eikenella
Kingella
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21
Q

what condition are the HACEK group commonly the cause of?

A

endocarditis

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22
Q 
oxidase +
growth on BAP (48-72hr)
catalase +
MAC -
HE -
BCYE +
urease +
intracellular
A

Brucella spp

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23
Q 
oxidase +
growth on BAP (48-72hr)
catalase +
extracellular
safety pin appearance
indole +
ornithine +
capnophilic
A

Pasteurella multocida

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24
Q

what organisms require cholesterol in order to grow?

A

Mycoplasma

Ureaplasma

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25
Q 
which Brucella spp matches these reactions:
CO2 +
H2S +
Thionine +
basic fuschin -
A

abortus (cow)

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26
Q 
which Brucella spp matches these reactions:
CO2 -
H2S +/-
Thionine -
basic fuschin +
A

suis (pigs)

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27
Q 
which Brucella spp matches these reactions:
CO2 -
H2S -
thionine -
basic fuschin -
A

melentensis (goats)

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28
Q

what is the mode of transmission for F. tularensis?

A

tick bite

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29
Q

what are the environmental factors required for fastidiuous organisms to grow?

A

5 - 10% CO2

30
Q 
Catalase -
oxidase -
hippurate +
starch +
10% KOH = fishy odor
Diffuse hemolysis on human blood plates 
Requires increased CO2 48 - 72 hr incubation
A

gardnerella vaginalis

31
Q

what organism will grow in hemolytic zones around staph?

A

haemophilus

32
Q 
oxidase -
catalase -
indole -
gliding motility
Increased CO2
Ferment g/l/s
A

capnocytophaga

33
Q

what organism causes a positive cold agglutinin titer?

A

M. pneumoniae

34
Q

Normal flora nonpathogenic haemophilus spp

A

Parainfluenzae
Haemolyticus
Parahaemolyticus

35
Q

H ducreyi infections

A

Chancroid

36
Q

Bordetella pertussis infections

A

Whooping cough

37
Q

Brucellosis infections

A

Brucellosis (undulant fever)

38
Q

Brucellosis transmission

A

Unpasteurized milk

Contact with infected goats, cows, hogs dogs

39
Q

Francisella infections

A

Tularemia

40
Q

Francisella transmission

A

Tick or infected animal

41
Q

Gardernella infections

A 
Bacterial vaginosis (no lactobacillus)
Bacteremia
42
Q

Legionella infection

A

Legionnaires disease

Pontiac fever

43
Q

Legionella transmission

A

Found in water

Inhalation of aerosol

44
Q

Pasteurella infections

A

Wound and respiratory tract infections

45
Q

Pasteurella transmission

A

Bite or scratch of cat or dog

46
Q

What reactions can be expected when para is in the species name for haemophilus

A

Require V factor

ALA +

47
Q

What reaction is expected on rabbit and horse blood agar with haemolyticus spp of haemophilus

A

Hemolysis

48
Q

Medium of choice for francisella tularensis

A

Cysteine blood agar

49
Q

factor X

A

hemin

50
Q

factor V

A

NAD

51
Q

why do haemophilus species that require X and V factors grow around staph species on BAP?

A

staph synthesizes NAD and hemin is released upon beta hemolysis

52
Q

X and V

no hemolysis

A

influenzae

aegyptius

53
Q

V factor

no hemolysis

A

parainfluenzae

54
Q

X and V

hemolysis

A

haemolyticus

55
Q

V factor

hemolysis

A

parahemolyticus

56
Q

X factor only

A

ducreyi

57
Q

methods to detect legionella

A

culture
DFA
serological methods

58
Q

pertussis swab collection

A

dacron

59
Q

obligate parasites

A

bordetella
brucella
bartonella

60
Q

brucella agar ingredients

A

casein
animal tissue
yeast
sodium bisulfite

61
Q

what is the special media required for francisella?

A

blood cysteine glucose agar with thiamine

62
Q

streptobacillus infection

A

rat bite fever

Haverhill fever

63
Q

HACEK group characteristics

A

colonize oropharynx

enhanced growth under increased CO2

64
Q

differentiate Neisseria and Kingella

A

N- Catalase +

K- catalase -

65
Q

organisms associated with animals

A

brucella
pasteurella
capnocytophaga
francisella

66
Q

H. influenzae disease

A

meningitis
epiglottitis
penumonia <5 >60
pink eye

67
Q

school of fish

A

H ducreyi

68
Q

bartonella infections

A

cat scratch fever
trench fever
carrions disease

69
Q

Differentiate h aegyptius and h influenzae

A

A - indole and xylose -

I- indole and xylose +

70
Q

How long does Legionella media need to be incubated?

A

At least 7 days

Microbiology (17 decks)

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