FASTIDIOUS GNR Flashcards
OXIDASE - CATALASE + UREASE - INDOLE - NITRATE + BHI agar- STAR SHAPED AREAS ON COLONIES
Actinobacillus actinomycetemcomitans
oxidase - catalase + G, MF TM + BCYE + no growth on blood Small yellow green growth Bipolar staining
Francisella tularensis
oxidase - catalase - growth on blood Rats fried egg appearance
streptobacillus
oxidase - catalase - no growth on blood urease + capnophilic
Ureaplasma urealyticum
oxidase - catalase - no growth BAP urease - arginine + fried eggs appearance
Mycoplasma hominis
oxidase - catalase - no growth on BAP urease - arginine -
Mycoplasma pneumoniae
oxidase + no growth on BAP Bordet Gengou (drops of mercury) Regan Lowe (mother of pearl) beta hemolytic Urea +
Bordetella pertussis
oxidase + catalase + gelatinase + motile no growth on BAP L-cysteine BCYE ground glass appearance
Legionella pneumophila
OXIDASE + no growth on BAP chocolate agar growth require X factor only (hemin) catalase - ALA-porph -
Haemophilus ducreyi
OXIDASE + no growth on BAP chocolate agar growth req V factor only (NAD) ALA porph + catalase -
Haemophilus paraphrophilus
OXIDASE + no growth on BAP chocolate agar growth Req V only ALA proph + catalase + gamma hemolysis
Haemophilus parainfluenzae
OXIDASE + no growth on BAP chocolate agar growth Req V only ALA proph + catalase + beta hemolysis rabbit/ horse blood
Haemophilus parahaemolyticus
OXIDASE + no growth on BAP chocolate agar growth Req X & V ALA proph - beta hemolysis
Haemophilus Haemolyticus
OXIDASE + no growth on BAP chocolate agar growth 5-10% CO2 Req X & V ALA proph - catalase + gamma hemolysis
Haemophilus influenzae
growth on BAP (48-72hr) catalse - indole - Oxidase + Nitrate +
Eikenella
what organism pits the agar, has a bleach odor, and is common in IV drug users?
Eikenella
growth on BAP (48-72hr) catalse - lactose fermenter ONPG + ALA-porpho +
Haemophilus aphrophilus
growth on BAP (48-72hr) oxidase + catalase - NLF ONPG - slow indole + nitrate - capnophilic
Cardiobacterium hominis
growth on BAP beta hemolytic (48-72hr) GF oxidase + catalase - NLF indole - H2S - can grow on Neisseria media
Kingella
what are the members of the HACEK group?
Haemophilus Actinobacillus / aggregatibacter Cardiobacterium Eikenella Kingella
what condition are the HACEK group commonly the cause of?
endocarditis
oxidase + growth on BAP (48-72hr) catalase + MAC - HE - BCYE + urease + intracellular
Brucella spp
oxidase + growth on BAP (48-72hr) catalase + extracellular safety pin appearance indole + ornithine + capnophilic
Pasteurella multocida
what organisms require cholesterol in order to grow?
Mycoplasma
Ureaplasma
which Brucella spp matches these reactions: CO2 + H2S + Thionine + basic fuschin -
abortus (cow)
which Brucella spp matches these reactions: CO2 - H2S +/- Thionine - basic fuschin +
suis (pigs)
which Brucella spp matches these reactions: CO2 - H2S - thionine - basic fuschin -
melentensis (goats)
what is the mode of transmission for F. tularensis?
tick bite
what are the environmental factors required for fastidiuous organisms to grow?
5 - 10% CO2
Catalase - oxidase - hippurate + starch + 10% KOH = fishy odor Diffuse hemolysis on human blood plates Requires increased CO2 48 - 72 hr incubation
gardnerella vaginalis
what organism will grow in hemolytic zones around staph?
haemophilus
oxidase - catalase - indole - gliding motility Increased CO2 Ferment g/l/s
capnocytophaga
what organism causes a positive cold agglutinin titer?
M. pneumoniae
Normal flora nonpathogenic haemophilus spp
Parainfluenzae
Haemolyticus
Parahaemolyticus
H ducreyi infections
Chancroid
Bordetella pertussis infections
Whooping cough
Brucellosis infections
Brucellosis (undulant fever)
Brucellosis transmission
Unpasteurized milk
Contact with infected goats, cows, hogs dogs
Francisella infections
Tularemia
Francisella transmission
Tick or infected animal
Gardernella infections
Bacterial vaginosis (no lactobacillus) Bacteremia
Legionella infection
Legionnaires disease
Pontiac fever
Legionella transmission
Found in water
Inhalation of aerosol
Pasteurella infections
Wound and respiratory tract infections
Pasteurella transmission
Bite or scratch of cat or dog
What reactions can be expected when para is in the species name for haemophilus
Require V factor
ALA +
What reaction is expected on rabbit and horse blood agar with haemolyticus spp of haemophilus
Hemolysis
Medium of choice for francisella tularensis
Cysteine blood agar
factor X
hemin
factor V
NAD
why do haemophilus species that require X and V factors grow around staph species on BAP?
staph synthesizes NAD and hemin is released upon beta hemolysis
X and V
no hemolysis
influenzae
aegyptius
V factor
no hemolysis
parainfluenzae
X and V
hemolysis
haemolyticus
V factor
hemolysis
parahemolyticus
X factor only
ducreyi
methods to detect legionella
culture
DFA
serological methods
pertussis swab collection
dacron
obligate parasites
bordetella
brucella
bartonella
brucella agar ingredients
casein
animal tissue
yeast
sodium bisulfite
what is the special media required for francisella?
blood cysteine glucose agar with thiamine
streptobacillus infection
rat bite fever
Haverhill fever
HACEK group characteristics
colonize oropharynx
enhanced growth under increased CO2
differentiate Neisseria and Kingella
N- Catalase +
K- catalase -
organisms associated with animals
brucella
pasteurella
capnocytophaga
francisella
H. influenzae disease
meningitis
epiglottitis
penumonia <5 >60
pink eye
school of fish
H ducreyi
bartonella infections
cat scratch fever
trench fever
carrions disease
Differentiate h aegyptius and h influenzae
A - indole and xylose -
I- indole and xylose +
How long does Legionella media need to be incubated?
At least 7 days