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Microbiology > Anaerobes > Flashcards

Anaerobes Flashcards

1
Q 
Vanc R
GNC
nitrate -
Kana S
Colis S
Red fluorescence
A

Veillonella

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2
Q 
Vanc R
GNR
BBE +
urea -
20% bile +
Kana & Colis S
A

Bacteroides gracilis

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3
Q 
Vanc R
GNR
BBE +
urea -
20% bile +
Kana & Colis R
LKV +
concentric circles on agar
A

Bacteroides fragilis

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4
Q 
Vanc R
GNR
BBE +
urea +
Kana & Colis S
nitrate +
catalase -
20% bile -
A

Bacteroides ureolyticus

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5
Q 
Vanc R
GNR
BBE +
UREA +
Kana & Colis S
Nitrate +
catalase +
20% bile +
fish eye colonies on BBE
A

Bilophila

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6
Q 
Vanc R
GNR
BBE -
Kanamycin R
brick red fluorescence
Colis S
fermenter
LKV +
A

Prevotella

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7
Q 
Vanc S
GNR
BBE -
Kanamycin R
brick red fluorescence
Colis R
nonfermenter
LKV -
A

Porphyromonas

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8
Q 
Vanc R
GNR
BBE -
Kana & Colis S
lipase +
indole +
pointed ends
A

Fusobacterium necrophorum

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9
Q 
Vanc R
GNR BBE -
Kana & Colis S 
lipase -
tapered ends
A

Fusobacterium nucleatum

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10
Q

Vanc S
GPC
Metro R
Novo R

A

Streptococcus intermedius

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11
Q

Vanc S
GPC
Metro R
Novo S

A

Staphylococcus saccharolyticus

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12
Q 
Vanc S
GPC
Metro S
Kana S
indole +
SPS R
Musty odor
A

Peptostreptococcus asaccharolyticus

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13
Q 
Vanc S 
GPC
Metro S
Kana R
SPS S
indole -
Sweet odor
A

Peptostreptococcus anaerobius

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14
Q 
Vanc S
GPR
non spore forming
motile
gull winged
A

Mobiluncus

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15
Q 
Vanc S
GPR
non spore forming
nonmotile
indole +
catalase +
Colis R
nitrate +
A

Propionibacterium acnes

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16
Q 
Vanc S
GPR
terminal spore 
horse stable odor
ground glass
chartreuse fluorescence
lecithinase -
CCFA + yellow
A

Clostridium difficile

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17
Q 
Vanc S 
GPR 
spore
lecithinase +
reverse CAMP +
box cars 
double zone beta hemolysis
A

Clostridium perfringens

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18
Q 
Vanc S 
GPR 
spore
lecithinase +
reverse CAMP -
lipase +
A

Clostridium botulinum

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19
Q 
Vanc S 
GPR
spore
lecithinase +
reverse CAMP -
lipase -
non swarming
A

Clostridium ramosum

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20
Q 
Vanc S
GPR
terminal spore tennis racket (round) 
Lecithinase -
reverse CAMP -
lipase -
indole and gelatinase +
narrow zone beta hemolysis
A

Clostridium tetani

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21
Q 
Vanc S
GPR
spore
Lecithinase +
reverse CAMP -
lipase -
A

Clostridium septicum

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22
Q

what are common characteristics of all Clostridium species?

A

catalse -
Kana S
Colis R
can look G-

23
Q

where are anaerobes normal flora?

A 
oropharynx
LGI
vagina
cervix
urethra
genitalia
24
Q

Acceptable specimens for anaerobic culture

A 
Bile
Blood
Body fluids
Bone marrow
Lung aspirate / biopsy
Suprapubic bladder aspirates 
Tissue
Transtracheal aspirate
Wound
25
Q

Ideal atmosphere

A

80 - 90% N2
5% H
5 - 10% CO2

26
Q

Anaerobic transport media

A

Tubes of O2 reduced agar

Gas impermeable bags

27
Q

Gold standard anaerobic method

A

Anaerobic chamber or glove box

28
Q

Peptostreptococcus anaerobius infections

A

Mixed infections of skin, soft tissues, GI, female genital tract, bones, joints, lungs, brain

29
Q

C diff infections

A

Antibiotic associated diarrhea

Pseudomembranous colitis

30
Q

C perfringens infections

A

Gas gangrene

Food poisoning

31
Q

Propionibacterium infections

A 
Acne
Artificial joint infections
Catheters
Shunts
Artificial heart valves
Keratitis
Bacteremia
Endocarditis
32
Q

GPR
AEROTOLERANT
CAT -

A

Lactobacillus

33
Q

What is used to fix gram stain specimens for anaerobes?

A

methanol

34
Q

what is a sign that oxygen has entered the anaerobic jar method?

A

indicator strip stays blue

35
Q

environment standards for anaerobe incubation

A

35 - 37
At least 48 hours
hold for at least 2-5 days

36
Q

lipase + organisms

A

Fusobacterium necrophorum
Prevotella intermedia
Clostridium botulinum

37
Q

what test can differentiate Peptostreptococcus anaerobius from other G+ cocci

A

SPS (Pepto = S)

38
Q

what is diameter is considered sensitive to SPS?

A

12 mm

39
Q

what does a positive lecithinase look like?

A

opaque white halos

40
Q

what is the only indole negative GNR

A

Bacteroides fragilis

41
Q

bile esculin + GNR

A

Bacteroides fragilis

B. gracilis

42
Q

differentiate bile esculin + GNR

A

fragilis- Kana and colistin R

gracilis- kana and colistin S

43
Q

only catalase + GNR

A

B. fragilis

44
Q

Starch + and DNase + GNR

A

Prevotella

45
Q

GNR fluorescers

A

fusobacterium
prevotella
porphyromonas

46
Q

differentiate prevotella and porphyromonas

A

Prevotella- Vanc R, Colistin S, fermenter, LKV+

Porphyromonas- Vanc S, Colistin R, nonfermenter, LKV-

47
Q

organism that causes Vincents angina or trench mouth

A

F. necrophorum

48
Q

clostridia exotoxins

A 
tetanospasmin
botulism
hemolysins
lecithinase
protease
collagenase
enterotoxin
49
Q

most common types of botulism toxin

A

B, A, E

50
Q

only clostridia that does not ferment glucose

A

tetani

51
Q

Vancomycin S

A

Porphyromonas

All G+ cocci and rods

52
Q

catalase -
indole -
SPS R
GPC

A

Finegoldia magna

53
Q

Only organism Vanc R, Kana R, Colis R

A

Bacteroides fragilis

54
Q

Fish eye colony

Appendicitis

A

Bilophila

Microbiology (17 decks)

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