Anaerobes Flashcards
1
Q
Vanc R GNC nitrate - Kana S Colis S Red fluorescence
A
Veillonella
2
Q
Vanc R GNR BBE + urea - 20% bile + Kana & Colis S
A
Bacteroides gracilis
3
Q
Vanc R GNR BBE + urea - 20% bile + Kana & Colis R LKV + concentric circles on agar
A
Bacteroides fragilis
4
Q
Vanc R GNR BBE + urea + Kana & Colis S nitrate + catalase - 20% bile -
A
Bacteroides ureolyticus
5
Q
Vanc R GNR BBE + UREA + Kana & Colis S Nitrate + catalase + 20% bile + fish eye colonies on BBE
A
Bilophila
6
Q
Vanc R GNR BBE - Kanamycin R brick red fluorescence Colis S fermenter LKV +
A
Prevotella
7
Q
Vanc S GNR BBE - Kanamycin R brick red fluorescence Colis R nonfermenter LKV -
A
Porphyromonas
8
Q
Vanc R GNR BBE - Kana & Colis S lipase + indole + pointed ends
A
Fusobacterium necrophorum
9
Q
Vanc R GNR BBE - Kana & Colis S lipase - tapered ends
A
Fusobacterium nucleatum
10
Q
Vanc S
GPC
Metro R
Novo R
A
Streptococcus intermedius
11
Q
Vanc S
GPC
Metro R
Novo S
A
Staphylococcus saccharolyticus
12
Q
Vanc S GPC Metro S Kana S indole + SPS R Musty odor
A
Peptostreptococcus asaccharolyticus
13
Q
Vanc S GPC Metro S Kana R SPS S indole - Sweet odor
A
Peptostreptococcus anaerobius
14
Q
Vanc S GPR non spore forming motile gull winged
A
Mobiluncus
15
Q
Vanc S GPR non spore forming nonmotile indole + catalase + Colis R nitrate +
A
Propionibacterium acnes
16
Q
Vanc S GPR terminal spore horse stable odor ground glass chartreuse fluorescence lecithinase - CCFA + yellow
A
Clostridium difficile
17
Q
Vanc S GPR spore lecithinase + reverse CAMP + box cars double zone beta hemolysis
A
Clostridium perfringens
18
Q
Vanc S GPR spore lecithinase + reverse CAMP - lipase +
A
Clostridium botulinum
19
Q
Vanc S GPR spore lecithinase + reverse CAMP - lipase - non swarming
A
Clostridium ramosum
20
Q
Vanc S GPR terminal spore tennis racket (round) Lecithinase - reverse CAMP - lipase - indole and gelatinase + narrow zone beta hemolysis
A
Clostridium tetani
21
Q
Vanc S GPR spore Lecithinase + reverse CAMP - lipase -
A
Clostridium septicum
22
Q
what are common characteristics of all Clostridium species?
A
catalse -
Kana S
Colis R
can look G-
23
Q
where are anaerobes normal flora?
A
oropharynx LGI vagina cervix urethra genitalia
24
Q
Acceptable specimens for anaerobic culture
A
Bile Blood Body fluids Bone marrow Lung aspirate / biopsy Suprapubic bladder aspirates Tissue Transtracheal aspirate Wound
25
Ideal atmosphere
80 - 90% N2
5% H
5 - 10% CO2
26
Anaerobic transport media
Tubes of O2 reduced agar
| Gas impermeable bags
27
Gold standard anaerobic method
Anaerobic chamber or glove box
28
Peptostreptococcus anaerobius infections
Mixed infections of skin, soft tissues, GI, female genital tract, bones, joints, lungs, brain
29
C diff infections
Antibiotic associated diarrhea
| Pseudomembranous colitis
30
C perfringens infections
Gas gangrene
| Food poisoning
31
Propionibacterium infections
```
Acne
Artificial joint infections
Catheters
Shunts
Artificial heart valves
Keratitis
Bacteremia
Endocarditis
```
32
GPR
AEROTOLERANT
CAT -
Lactobacillus
33
What is used to fix gram stain specimens for anaerobes?
methanol
34
what is a sign that oxygen has entered the anaerobic jar method?
indicator strip stays blue
35
environment standards for anaerobe incubation
35 - 37
At least 48 hours
hold for at least 2-5 days
36
lipase + organisms
Fusobacterium necrophorum
Prevotella intermedia
Clostridium botulinum
37
what test can differentiate Peptostreptococcus anaerobius from other G+ cocci
SPS (Pepto = S)
38
what is diameter is considered sensitive to SPS?
12 mm
39
what does a positive lecithinase look like?
opaque white halos
40
what is the only indole negative GNR
Bacteroides fragilis
41
bile esculin + GNR
Bacteroides fragilis
| B. gracilis
42
differentiate bile esculin + GNR
fragilis- Kana and colistin R
| gracilis- kana and colistin S
43
only catalase + GNR
B. fragilis
44
Starch + and DNase + GNR
Prevotella
45
GNR fluorescers
fusobacterium
prevotella
porphyromonas
46
differentiate prevotella and porphyromonas
Prevotella- Vanc R, Colistin S, fermenter, LKV+
| Porphyromonas- Vanc S, Colistin R, nonfermenter, LKV-
47
organism that causes Vincents angina or trench mouth
F. necrophorum
48
clostridia exotoxins
```
tetanospasmin
botulism
hemolysins
lecithinase
protease
collagenase
enterotoxin
```
49
most common types of botulism toxin
B, A, E
50
only clostridia that does not ferment glucose
tetani
51
Vancomycin S
Porphyromonas
| All G+ cocci and rods
52
catalase -
indole -
SPS R
GPC
Finegoldia magna
53
Only organism Vanc R, Kana R, Colis R
Bacteroides fragilis
54
Fish eye colony
| Appendicitis
Bilophila
